Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF)

Almeida, J. R. M. de; Modig, Tobias; Röder, A.; Lidén, Gunnar; Gorwa-Grauslund, Marie-Françoise

doi:10.1186/1754-6834-1-12

Cited by 65 publications

(47 citation statements)

References 38 publications

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“…In the present investigation, HMF and furfural were completely metabolized by the strain before significant utilization of sugars started. This was also previously reported by others (Almeida et al 2008; Wan et al 2012) and indicates that S. stipitis CBS6054 is readily capable of converting HMF and furfural in the tested lignocellulose hydrolysate from sugarcane bagasse. Cell growth was highest (0.079 g/l/h) in hydrolysate containing mixed sugars and inhibitors such as acetate, HMF and furfural at concentrations of 3.2 ± 0.1, 0.4 and 0.5 g/l, respectively, indicating that the processing of bagasse hydrolysate under this condition will not inhibit the growth of S. stipitis .…”

Section: Discussionsupporting

confidence: 89%

“…However, previous studies have shown arabinose is only utilized by S. stipitis for cell growth but not for ethanol production (Nigam 2001b). Furthermore, S. stipitis also has the natural ability to metabolize some of the sugar degradation compounds present in the hydrolysate after pretreatment (Almeida et al 2008; Wan et al 2012). The sensitivity of Scheffersomyces stipitis to inhibitors found in lignocellulose hydrolysate has been reported elsewhere (Bellido et al 2011; Delgenes et al 1996).…”

Section: Introductionmentioning

confidence: 99%

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Conversion of C6 and C5 sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054

2013

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Sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. This stresses the importance of utilizing both C6 and C5 sugars for conversion into ethanol in order to improve the process economics. During processing of the hydrolysate degradation products such as acetate, 5-hydroxymethylfurfural (HMF) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. In the current study, conversion of both glucose and xylose sugars into ethanol in wet exploded bagasse hydrolysates was investigated without detoxification using Scheffersomyces (Pichia) stipitis CBS6054, a native xylose utilizing yeast strain. The sugar utilization ratio and ethanol yield (Yp/s) ranged from 88-100% and 0.33-0.41 ± 0.02 g/g, respectively, in all the hydrolysates tested. Hydrolysate after wet explosion at 185°C and 6 bar O2, composed of mixed sugars (glucose and xylose) and inhibitors such as acetate, HMF and furfural at concentrations of 3.2 ± 0.1, 0.4 and 0.5 g/l, respectively, exhibited highest cell growth rate of 0.079 g/l/h and an ethanol yield of 0.39 ± 0.02 g/g sugar converted. Scheffersomyces stipitis exhibited prolonged fermentation time on bagasse hydrolysate after wet explosion at 200°C and 6 bar O2 where the inhibitors concentration was further increased. Nonetheless, ethanol was produced up to 18.7 ± 1.1 g/l resulting in a yield of 0.38 ± 0.02 g/g after 82 h of fermentation.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Conversion of C6 and C5 sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054

2013

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“…If this were the case, then this gene cluster would be downregulated because the adapted strains have a reduced consumption of xylose. However, given that xylose reductase has been demonstrated in aiding detoxiWcation processes, it is unlikely that downregulation would be advantageous for P. stipitis survival in a concentrated wood hydrolysate [2]. Therefore, it is doubtful that xylose reductase caused the observed change in xylose metabolism in P. stipitis RS01, RS02, and RS03.…”

Section: Carbohydrate Utilization Of Adapted Strainsmentioning

confidence: 96%

“…The xylose reductase has previously been established as an enzyme involved in detoxiWcation processes in P. stipitis and, therefore, may be related to changes observed in the adapted strains [2]. However, because there are multiple copies of the gene cluster encoding the xylose reductase gene (aldo/keto reductase) only a regulatory change would have resulted in the observed decrease in xylose utilization [13].…”

Section: Carbohydrate Utilization Of Adapted Strainsmentioning

confidence: 97%

Overcoming inhibitors in a hemicellulosic hydrolysate: improving fermentability by feedstock detoxification and adaptation of Pichia stipitis

Stoutenburg

Perrotta

Nakas

2011

J Ind Microbiol Biotechnol

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In order to improve the fermentative efficiency of sugar maple hemicellulosic hydrolysates for fuel ethanol production, various methods to mitigate the effects of inhibitory compounds were employed. These methods included detoxification treatments utilizing activated charcoal, anion exchange resin, overliming, and ethyl acetate extraction. Results demonstrated the greatest fermentative improvement of 50% wood hydrolysate (v/v) by Pichia stipitis with activated charcoal treatment. Another method employed to reduce inhibition was an adaptation procedure to produce P. stipitis stains more tolerant of inhibitory compounds. This adaptation resulted in yeast variants capable of improved fermentation of 75% untreated wood hydrolysate (v/v), one of which produced 9.8 g/l ± 0.6 ethanol, whereas the parent strain produced 0.0 g/l ± 0.0 within the first 24 h. Adapted strains RS01, RS02, and RS03 were analyzed for glucose and xylose utilization and results demonstrated increased glucose and decreased xylose utilization rates in comparison to the wild type. These changes in carbohydrate utilization may be indicative of detoxification or tolerance activities related to proteins involved in glucose and xylose metabolism.

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“…The ability to use a wider array of the biomass feedstocks would help to decrease cost by reducing the number of upstream processing steps and by turning more of the biomass into biofuel. For example, S. cerevisiae has been engineered with the genes encoding xylose reductase (Xyllp) and xylitol dehydrogenase (Xyl2p) from Pichia stipitis to enable it to utilize xylose, the second most abundant carbohydrate in nature, as a carbon source for ethanol production [ 39]. However, simply overexpressing the genes led to low growth and fermentation rates due to redox imbalance.…”

Section: Production Hostmentioning

confidence: 99%

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

Lee

Chou

Ham

et al. 2008

Current Opinion in Biotechnology

554

302

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The ability to generate microorganisms that can produce biofuels similar to petroleum-based transportation fuels would allow the use of existing engines and infrastructure and would save an enormous amount of capital required for replacing the current infrastructure to accommodate biofuels that have properties significantly different from petroleum-based fuels. Several groups have demonstrated the feasibility of manipulating microbes to produce molecules similar to petroleum-derived products, albeit at relatively low productivity (e.g. maximum butanol production is around 20 g/L). For cost-effective production of biofuels, the fuel-producing hosts and pathways must be engineered and optimized. Advances in metabolic engineering and synthetic biology will provide new tools for metabolic engineers to better understand how to rewire the cell in order to create the desired phenotypes for the production of economically viable biofuels.

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Pichia stipitis xylose reductase helps detoxifying lignocellulosic hydrolysate by reducing 5-hydroxymethyl-furfural (HMF)

Cited by 65 publications

References 38 publications

Conversion of C6 and C5 sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054

Conversion of C6 and C5 sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054

Overcoming inhibitors in a hemicellulosic hydrolysate: improving fermentability by feedstock detoxification and adaptation of Pichia stipitis

Metabolic engineering of microorganisms for biofuels production: from bugs to synthetic biology to fuels

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