This study investigated the effects of wearing AR devices on users’ performance and comfort ratings while performing order-picking (OP) tasks. In addition to a picking-by-paper list, two AR devices combined with three order information display designs were examined. Thirty adult participants joined. They searched for and found the boxes in the order list, picked them up, and returned to the origin. The time to complete the task and the number of incorrect boxes picked up were analyzed to assess the performance of the tasks. The subjective ratings of the participants on the comfort rating scale (CRS) and the simulator sickness questionnaire (SSQ) were collected to assess the designs of both the AR devices and the order information displays. It was found that the participants could complete the OP tasks faster when adopting the order map (4.97 ± 1.57 min) or the 3D graph display (4.87 ± 1.50 min) using either one of the AR devices than when using a paper list (6.03 ± 1.28 min). However, they needed more time to complete the OP tasks when wearing both types of AR glasses when the Quick Response (QR) code option was adopted (10.16 ± 4.30 min) than when using a paper list. The QR code scanning and display design using either one of the AR devices guaranteed 100% accuracy but sacrificed efficiency in task completion. The AR device with a binocular display and hand gesture recognition functions had a significantly lower CRS score in the dimensions of attachment and movement (5.6 and 6.3, respectively) than the corresponding dimensions (8.0 and 8.3, respectively) of the other device with a monocular and hand touch input design. There were complaints of eye strain after using both AR devices examined in this study. This implies that these AR devices may not be suitable to wear for extended periods of time. Users should take off the AR device whenever they do not need to view the virtual image to avoid eye strain and other discomfort symptoms.