PICKLE is a CHD3 chromatin-remodeling factor that regulates the transition from embryonic to vegetative development in
            <i>Arabidopsis</i>

Ogas, Joe; Kaufmann, Scott H.; Henderson, Jim T.; Somerville, Chris

doi:10.1073/pnas.96.24.13839

Cited by 461 publications

(416 citation statements)

References 29 publications

Supporting

Mentioning

395

Contrasting

Unclassified

Order By: Relevance

“…WUS function is not directly linked to embryo identity, but rather to the maintenance of an undifferentiated cell state that responds to different stimuli to change the developmental fate of tissues (Gallois et al 2002(Gallois et al , 2004. Loss-of-function mutants of the PICKLE (PKL) CHD3 chromatin-remodeling factor convert seedling roots into embryogenic tissues (Ogas et al 1999). PKL activity is not restricted to seedlings, as pkl mutants also exhibit GA-deficient shoot phenotypes (Ogas et al 1997;Henderson et al 2004), as well as ectopic activation of primordium and meristem identity genes in the carpel (Eshed et al 1999).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Passarinho¹,

Ketelaar²,

Xing³

et al. 2008

Plant Mol Biol

106

View full text Add to dashboard Cite

Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth.

show abstract

Section: Introductionmentioning

confidence: 99%

“…It is becoming clear that the developmental pathways in which some of these embryo promoting/repressing genes function are interconnected (Ogas et al 1999;Zuo et al 2002;Rider et al 2003;Harding et al 2003). Dissecting the complexity of these networks is a major challenge that requires the detailed analysis of each individual pathway.…”

Section: Introductionmentioning

confidence: 99%

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Passarinho¹,

Ketelaar²,

Xing³

et al. 2008

Plant Mol Biol

106

View full text Add to dashboard Cite

show abstract

“…PKL was shown to regulate the transition from embryonic to vegetative development in Arabidopsis (Ogas et al, 1999). pkl mutant plants exhibit some GA-deficient phenotypes, including dark green leaves with short petioles, delayed bolting and reduced apical dominance (Henderson et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In Arabidopsis, PICKLE (PKL) encodes a CHD3-like ATP-dependent chromatin remodeling factor (Ogas et al, 1997(Ogas et al, , 1999Henderson et al, 2004;Ho et al, 2013). PKL was shown to regulate the transition from embryonic to vegetative development in Arabidopsis (Ogas et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis

Liang

et al. 2016

Sci. China Life Sci.

View full text Add to dashboard Cite

PICKLE (PKL), a putative CHD3 chromatin remodeling factor, has been suggested to be involved in multiple processes in Arabidopsis. Here, we confirmed the late-flowering phenotype caused by pkl mutation with pkl mutants in two different ecotypes, and investigated the possible mechanisms that account for PKL regulation of flowering time. Quantitative RT-PCR and RNA-seq assays showed that expression of the LEAFY gene (LFY) and a number of LFY-regulated floral homeotic genes were down-regulated in seedlings of the pkl mutants. As predicted, overexpression of LFY restored normal flowering time of pkl mutants. Our results suggest that PKL may be involved in regulating flowering time via LFY expression. To uncover the underlying mechanism, ChIP-PCR using anti-PKL was performed on materials from three developmental stages of seedlings. Our results showed that PKL associated with the genomic sequences of LFY, particularly at 10-day and 25-day after germination. We also showed that loss of PKL affected H3K27me3 level at the promoter of LFY. Taken together, our data suggest that transcriptional regulation of LFY at the chromatin level by PKL may at least partially account for the late-flowering phenotype of pkl mutants. chromatin-remodeling factor, PICKLE, floral transition, LEAFY, Arabidopsis Citation:Fu, X., Li, C., Liang, Q., Zhou, Y., He, H., and Fan, L.M. (2016). CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis. Sci China Life Sci 59, 516 -528.

show abstract

“…The first suppressor of LEC genes to be characterized was PICKLE (PKL), which encodes a putative chromatin-remodeling factor belonging to the CHROMODOMAIN/HELICASE/ DNA-BINDING3 (CHD3) family and regulates transition from embryonic to vegetative development (Ogas et al, 1999). In agreement with LEC overexpression phenotypes, pkl mutants accumulate storage macromolecules and give rise to embryogenic calli in culture.…”

Section: Discussionmentioning

confidence: 83%

Truncation of LEAFY COTYLEDON1 Protein Is Required for Asexual Reproduction in Kalanchoë daigremontiana

et al. 2014

View full text Add to dashboard Cite

Kalanchoë daigremontiana reproduces asexually by generating numerous plantlets on its leaf margins. The formation of plantlets requires the somatic initiation of organogenic and embryogenic developmental programs in the leaves. However, unlike normal embryogenesis in seeds, leaf somatic embryogenesis bypasses seed dormancy to form viable plantlets. In Arabidopsis (Arabidopsis thaliana), seed dormancy and embryogenesis are initiated by the transcription factor LEAFY COTYLEDON1 (LEC1). The K. daigremontiana ortholog of LEC1 is expressed during leaf somatic embryo development. However, KdLEC1 encodes for a LEC1-type protein that has a unique B domain, with 11 unique amino acids and a premature stop codon. Moreover, the truncated KdLEC1 protein is not functional in Arabidopsis. Here, we show that K. daigremontiana transgenic plants expressing a functional, chimeric KdLEC1 gene under the control of Arabidopsis LEC1 promoter caused several developmental defects to leaf somatic embryos, including seed dormancy characteristics. The dormant plantlets also behaved as typical dormant seeds. Transgenic plantlets accumulated oil bodies and responded to the abscisic acid biosynthesis inhibitor fluridone, which broke somatic-embryo dormancy and promoted their normal development. Our results indicate that having a mutated form of LEC1 gene in K. daigremontiana is essential to bypass dormancy in the leaf embryos and generate viable plantlets, suggesting that the loss of a functional LEC1 promotes viviparous leaf somatic embryos and thus enhances vegetative propagation in K. daigremontiana. Mutations resulting in truncated LEC1 proteins may have been of a selective advantage in creating somatic propagules, because such mutations occurred independently in several Kalanchoë species, which form plantlets constitutively.

show abstract

PICKLE is a CHD3 chromatin-remodeling factor that regulates the transition from embryonic to vegetative development in Arabidopsis

Cited by 461 publications

References 29 publications

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

CHD3 chromatin-remodeling factor PICKLE regulates floral transition partially via modulating LEAFY expression at the chromatin level in Arabidopsis

Truncation of LEAFY COTYLEDON1 Protein Is Required for Asexual Reproduction in Kalanchoë daigremontiana

Contact Info

Product

Resources

About