Picomolar-sensitive impedimetric sensor for salivary calcium analysis at POC based on SAM of Schiff base–modified gold electrode

Magar, Hend S.; Abbas, Mohammed Nooredeen; Ali, Mounir Ben; Ahmed, Mohamed M. Z.

doi:10.1007/s10008-020-04500-w

Cited by 29 publications

(18 citation statements)

References 56 publications

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“…The interface between electronic components and biological objects plays a crucial role in bioelectronics applications ranging from simple biosensors to neuroelectronics. − In the optimal case it should enable a stable mechanical coupling and a good electronic coupling between the chip and the biological objects. Typical strategies to improve this interface are based on the tailoring of the existing surface, for instance via morphological modification − or functionalization of the surface via an adequate coating.…”

Section: Introductionmentioning

confidence: 99%

Mechanical and Electronic Cell–Chip Interaction of APTES-Functionalized Neuroelectronic Interfaces

Wolf

Rai

Glass

et al. 2021

ACS Appl. Bio Mater.

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In this work, we analyze the impact of a chip coating with a self-assembled monolayer (SAM) of (3-aminopropyl)triethoxysilane (APTES) on the electronic and mechanical properties of neuroelectronic interfaces. We show that the large signal transfer, which has been observed for these interfaces, is most likely a consequence of the strong mechanical coupling between cells and substrate. On the one hand, we demonstrate that the impedance of the interface between Pt electrodes and an electrolyte is slightly reduced by the APTES SAM. However, this reduction of approximately 13% is definitely not sufficient to explain the large signal transfer of APTES coated electrodes demonstrated previously. On the other hand, the APTES coating leads to a stronger mechanical clamping of the cells, which is visible in microscopic images of the cell development of APTES-coated substrates. This stronger mechanical interaction is most likely caused by the positively charged amino functional group of the APTES SAM. It seems to lead to a smaller cleft between substrate and cells and, thus, to reduced losses of the cell’s action potential signal at the electrode. The disadvantage of this tight binding of the cells to the rigid, planar substrate seems to be the short lifetime of the cells. In our case the density of living cells starts to decrease together with the visual deformation of the cells typically at DIV 9. Solutions for this problem might be the use of soft substrates and/or the replacement of the short APTES molecules with larger molecules or molecular multilayers.

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Section: Introductionmentioning

confidence: 99%

Mechanical and Electronic Cell–Chip Interaction of APTES-Functionalized Neuroelectronic Interfaces

Wolf

Rai

Glass

et al. 2021

ACS Appl. Bio Mater.

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“…The interface between the usually inorganic electronic components and biological objects such as neurons plays a crucial role in the success of applications ranging from biosensors to bioelectronics. − Modifying the surface of substrates to tailor their surface properties (ranging from mechanical or electronic properties to bioaffinity) and thus optimizing them for a given application is a well-known strategy. For example, the functionalization of a conventional inorganic substrate such as SiO 2 via silanization is well understood. − However, bioelectronic components such as electrophysiological sensors are typically composed of several materials like insulating oxides and metals with very different surface properties.…”

Section: Introductionmentioning

confidence: 99%

Surface Functionalization of Platinum Electrodes with APTES for Bioelectronic Applications

Wolf

Yuan

Hassani

et al. 2020

ACS Appl. Bio Mater.

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The interface between electronic components and biological objects plays a crucial role in the success of bioelectronic devices. Since the electronics typically include different elements such as an insulating substrate in combination with conducting electrodes, an important issue of bioelectronics involves tailoring and optimizing the interface for any envisioned applications. In this paper, we present a method for functionalizing insulating substrates (SiO2) and metallic electrodes (Pt) simultaneously with a stable monolayer of organic molecules ((3-aminopropyl)triethoxysilane (APTES)). This monolayer is characterized by high molecule density, long-term stability, and positive surface net charge and most likely represents a self-assembled monolayer (SAM). It facilitates the conversion of biounfriendly Pt surfaces into biocompatible surfaces, which allows cell growth (neurons) on both functionalized components, SiO2 and Pt, which is comparable to that of reference samples coated with poly-L-lysine (PLL). Moreover, the functionalization greatly improves the electronic cell–chip coupling, thereby enabling the recording of action potential signals of several millivolts at APTES-functionalized Pt electrodes.

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“…In a recent study, an impedimetric sensor for total calcium detection was developed using a gold nanoparticle self-assembled monolayer to provide high sensitivity and a wide linear range from 5 × 10 −12 to 1 × 10 −6 mol L −1 with the low limit of detection of 3.6 × 10 −12 mol L −1 [ 45 ].…”

Section: Nanomaterials Influences the Impedimetric Biosensorsmentioning

confidence: 99%

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

Magar

Hassan

Mulchandani

2021

Sensors

598

238

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Electrochemical impedance spectroscopy (EIS) is a powerful technique used for the analysis of interfacial properties related to bio-recognition events occurring at the electrode surface, such as antibody–antigen recognition, substrate–enzyme interaction, or whole cell capturing. Thus, EIS could be exploited in several important biomedical diagnosis and environmental applications. However, the EIS is one of the most complex electrochemical methods, therefore, this review introduced the basic concepts and the theoretical background of the impedimetric technique along with the state of the art of the impedimetric biosensors and the impact of nanomaterials on the EIS performance. The use of nanomaterials such as nanoparticles, nanotubes, nanowires, and nanocomposites provided catalytic activity, enhanced sensing elements immobilization, promoted faster electron transfer, and increased reliability and accuracy of the reported EIS sensors. Thus, the EIS was used for the effective quantitative and qualitative detections of pathogens, DNA, cancer-associated biomarkers, etc. Through this review article, intensive literature review is provided to highlight the impact of nanomaterials on enhancing the analytical features of impedimetric biosensors.

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Picomolar-sensitive impedimetric sensor for salivary calcium analysis at POC based on SAM of Schiff base–modified gold electrode

Cited by 29 publications

References 56 publications

Mechanical and Electronic Cell–Chip Interaction of APTES-Functionalized Neuroelectronic Interfaces

Mechanical and Electronic Cell–Chip Interaction of APTES-Functionalized Neuroelectronic Interfaces

Surface Functionalization of Platinum Electrodes with APTES for Bioelectronic Applications

Electrochemical Impedance Spectroscopy (EIS): Principles, Construction, and Biosensing Applications

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