Picosecond absorbance changes upon selective excitation of the primary electron donor P-700 in Photosystem I

Шувалов, В.А.; Nuijs, Antonius M.; Gorkom, Hans J. van; Smit, Henk; Duysens, L.N.M.

doi:10.1016/0005-2728(86)90187-8

Cited by 124 publications

(92 citation statements)

References 8 publications

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“…So, the P-700 triplet produced by the charge recombination between P-700 + and Ao may be attributed as well. The yield of the triplet by the charge recombination between P-700 ÷ and Ao is suggested to be about 30% at room temperature [16]. The extent of the fast decay component is almost the same as that of the slow component in the photoinhibited preparation (Fig.…”

Section: Kinetics Of Optical Absorption Changementioning

confidence: 63%

Destruction of photosystem I iron‐sulfur centers in leaves of Cucumis sativus L. by weak illumination at chilling temperatures

et al. 1995

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Section: Kinetics Of Optical Absorption Changementioning

confidence: 63%

Destruction of photosystem I iron‐sulfur centers in leaves of Cucumis sativus L. by weak illumination at chilling temperatures

et al. 1995

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“…The limitation of electron transfer from P700 to downstream electron acceptors would suppress the charge separation between P700 and chlorophyll A 0 (Shuvalov et al, 1986). Furthermore, the production of the P700 triplet-state ( 3 P700) is initiated (Rutherford et al, 2012;Shuvalov et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

“…O 2 2 either directly attacks the peripheral component, or is converted to OH$ via the Fenton reaction between the iron-sulfur center in F X , F A , or F B in the diffusing process (Inoue et al, 1986;Sonoike et al, 1995;Takahashi and Asada, 1988). The limitation of electron transfer from P700 to downstream electron acceptors would suppress the charge separation between P700 and chlorophyll A 0 (Shuvalov et al, 1986). Furthermore, the production of the P700 triplet-state ( 3 P700) is initiated (Rutherford et al, 2012;Shuvalov et al, 1986).…”

Section: Discussionmentioning

confidence: 99%

Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition

et al. 2016

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Photosystem I (PSI) photoinhibition suppresses plant photosynthesis and growth. However, the mechanism underlying PSI photoinhibition has not been fully clarified. In this study, in order to investigate the mechanism of PSI photoinhibition in higher plants, we applied repetitive short-pulse (rSP) illumination, which causes PSI-specific photoinhibition in chloroplasts isolated from spinach leaves. We found that rSP treatment caused PSI photoinhibition, but not PSII photoinhibition in isolated chloroplasts in the presence of O 2 . However, chloroplastic superoxide dismutase and ascorbate peroxidase activities failed to protect PSI from its photoinhibition. Importantly, PSI photoinhibition was largely alleviated in the presence of methyl viologen, which stimulates the production of reactive oxygen species (ROS) at the stromal region by accepting electrons from PSI, even under the conditions where CuZn-superoxide dismutase and ascorbate peroxidase activities were inactivated by KCN. These results suggest that the ROS production site, but not the ROS production rate, is critical for PSI photoinhibition. Furthermore, we found that not only superoxide (O 2 2 ) but also singlet oxygen ( 1 O 2 ) is involved in PSI photoinhibition induced by rSP treatment. From these results, we suggest that PSI photoinhibition is caused by both O 2 2 and 1 O 2 produced within the thylakoid membranes when electron carriers in PSI become highly reduced. Here, we show, to our knowledge, new insight into the PSI photoinhibition in higher plants.

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“…These include Chl singlets and triplets, P-680, Ao, and phaeophytin (Doring et al, 1967;Klimov aet al, 1986;Nuijs et al, 1986aNuijs et al, , 1986b. However, most of these species are very short-lived (lO-" to l O w 7 s; Kramer and Mathis, 1980;Nuijs et al, 1986a;Shuvalov et al, 1986;Mathis et al, 1988;Schatz et al, 1988;Genty et al, 1992) and will not contribute to the absorbance change procluced by I'-700+, which has a lifetime of 10-3 to lO-'s (Haehnel, 1984) at the irradiances of up to 2000 to 3000 pmol m-'s-l used in whole-leaf photosynthesis experiments. The case with I'-680 is possibly more difficult.…”

Section: P-700 In Leavesmentioning

confidence: 99%

Changes in P-700 Oxidation during the Early Stages of the Induction of Photosynthesis

Harbinson

Hedley

1993

Plant Physiol.

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Following dark adaptation, the response to irradiance of chlorophyll (Chl) fluorescence, the light-induced absorbance change around 820 nm (to measure reaction center Chl of photosystem I[PSI] P-700 oxidation), and COz fixation were examined in pea (Pisum sativum 1.) leaves under a range of conditions. Initially, P-700 oxidation is restricted by a lack of regeneration of PSI electron acceptors, and the increase of oxidized P-700 (P-700+) that occurs during approximately the first 60 s of irradiation is largely independent of the resistance to electron flow between the two photosystems. Under these conditions, the quantum efficiency for linear electron flow is directly positively related to P-700+ accumulation, which is in contrast to the direct negative correlation that is the most frequently reported relationship between P-700+ accumulation and the quantum efficiency for linear electron flow.

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Picosecond absorbance changes upon selective excitation of the primary electron donor P-700 in Photosystem I

Cited by 124 publications

References 8 publications

Destruction of photosystem I iron‐sulfur centers in leaves of Cucumis sativus L. by weak illumination at chilling temperatures

Destruction of photosystem I iron‐sulfur centers in leaves of Cucumis sativus L. by weak illumination at chilling temperatures

Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition

Changes in P-700 Oxidation during the Early Stages of the Induction of Photosynthesis

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