With the rapid development of industrial technology, the application fields of AGV are constantly expanding. In this article, a differential vehicle is selected to construct a dynamic model of differential vehicle and establish a co-simulation platform of MATLAB/Simulink and ADAMS, which fully considers the nonlinear friction between wheels and the ground, the body mass and its own moment of inertia during steering, simulates the actual motion trajectory of the vehicle under different paths, and compares the ideal trajectory with the actual ADAMS output, which is generally consistent with the theory, and the basic path trend tends to be consistent. The deviation between them also reflects that the differential vehicle is a multi-degree-of-freedom strong nonlinear system, so the platform can better simulate the actual motion process of the vehicle.