2020
DOI: 10.1038/s41598-020-68477-2
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PiDose: an open-source system for accurate and automated oral drug administration to group-housed mice

Abstract: Drug treatment studies in laboratory mice typically employ manual administration methods such as injection or gavage, which can be time-consuming to perform over long periods and cause substantial stress in animals. These stress responses may mask or enhance treatment effects, increasing the risk of false positive or negative results and decreasing reliability. to address the lack of an automated method for drug treatment in group-housed mice, we have developed PiDose, a home-cage attached device that weighs i… Show more

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Cited by 10 publications
(9 citation statements)
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References 41 publications
(50 reference statements)
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“…Animal tissue was collected via ear clipping at weaning, and DNA extraction and PCR analysis were subsequently used to determine genotype. In order to enable automated identification of group-housed mice during behavioral testing, glass RFID capsules (Sparkfun SEN-09416) were subcutaneously implanted in the upper thoracic torso as previously described (Woodard et al, 2020). Mice were handled by the experimenter on at least two occasions for 2-3 min per animal before any surgery or behavioral testing.…”
Section: Animalsmentioning
confidence: 99%
“…Animal tissue was collected via ear clipping at weaning, and DNA extraction and PCR analysis were subsequently used to determine genotype. In order to enable automated identification of group-housed mice during behavioral testing, glass RFID capsules (Sparkfun SEN-09416) were subcutaneously implanted in the upper thoracic torso as previously described (Woodard et al, 2020). Mice were handled by the experimenter on at least two occasions for 2-3 min per animal before any surgery or behavioral testing.…”
Section: Animalsmentioning
confidence: 99%
“…The essential components of our system are widely available and affordable; we constructed four video systems at an approximate cost of $140 each. Although beyond the scope of this protocol, the system may also be readily extended to incorporate features such as automated radio frequency identification (RFID), weighing, individualized dosing, and operant conditioning tasks (e.g., Noorshams et al., 2017 ; Woodard et al., 2020 ).…”
Section: Before You Beginmentioning
confidence: 99%
“…In order to enable automated identification of group-housed mice during behavioural testing, glass RFID capsules (Sparkfun SEN-09416) were subcutaneously implanted in the upper thoracic torso as previously described (Woodard et al, 2020). Mice were handled by the experimenter on at least two occasions for 2-3 minutes per animal prior to any surgery or behavioural testing.…”
Section: Animalsmentioning
confidence: 99%