Piezo1 induces endothelial responses to shear stress via soluble adenylyl Cyclase-IP3R2 circuit

Nunez, Dianicha Santana; Malik, Asrar B.; Lee, Quinn; Ahn, Sang Joon; Coctecon-Murillo, Arnold; Lazarko, Dana; Levitan, Irena; Mehta, Dolly; Komarova, Yulia

doi:10.1016/j.isci.2023.106661

Cited by 8 publications

(3 citation statements)

References 86 publications

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“…For example, TRP proteins are integral to agonist and store depletion activated Ca 2+ entry channels that are in turn regulated directly by sarcoendoplasmic reticulum (SR) Ca 2+ release channels ( Sienaert et al, 1996 ; Boulay et al, 1999 ). Piezo1 can modulate SR Ca 2+ release dynamics via such channels ( Santana Nunez et al, 2023 ), and thus the SR may be a point of interaction. Piezo1 transduces increased myocardial forces into chemical signals that initiate hypertrophic signaling via a close physical interaction with TRPM4 ( Yu et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Mechanosensitive channels in lung disease

Zheng,

Borkar,

Yao

et al. 2023

Front. Physiol.

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Mechanosensitive channels (MS channels) are membrane proteins capable of responding to mechanical stress over a wide dynamic range of external mechanical stimuli. In recent years, it has been found that MS channels play an important role as “sentinels” in the process of cell sensing and response to extracellular and intracellular force signals. There is growing appreciation for mechanical activation of ion channels and their subsequent initiation of downstream signaling pathways. Members of the transient receptor potential (TRP) superfamily and Piezo channels are broadly expressed in human tissues and contribute to multiple cellular functions. Both TRP and Piezo channels are thought to play key roles in physiological homeostasis and pathophysiology of disease states including in the lung. Here, we review the current state of knowledge on the expression, regulation, and function of TRP and Piezo channels in the context of the adult lung across the age spectrum, and in lung diseases such as asthma, COPD and pulmonary fibrosis where mechanical forces likely play varied roles in the structural and functional changes characteristic of these diseases. Understanding of TRP and Piezo in the lung can provide insights into new targets for treatment of pulmonary disease.

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Section: Discussionmentioning

confidence: 99%

Mechanosensitive channels in lung disease

Zheng,

Borkar,

Yao

et al. 2023

Front. Physiol.

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“…This channel is sensitive to varying flow conditions, prompting calcium influx into ECs and subsequently initiating a cascade of downstream responses. 32 Figure 2 B shows Piezo1-mediated EC responses under laminar and turbulent flows. Under laminar flow conditions, the activation of the channel and subsequent calcium influx can lead to structural adaptations in the vessel, promoting physiological vascular remodeling.…”

Section: Influence Of Fluid Shear Stress On Vascularizationmentioning

confidence: 99%

“…Engineering physiologically relevant vascularization within organoids demands a comprehensive understanding of the mechanobiological environment governing EC behavior. While Piezo1’s role in shear stress sensing is well established, 32 , 35 a diverse network of mechanosensory proteins contributes to vascular development and remodeling. This network includes ion channels of the TRP family and potassium channels, which modulate flow-mediated dilation.…”

Section: Influence Of Fluid Shear Stress On Vascularizationmentioning

confidence: 99%

Bioengineering methods for vascularizing organoids

Nwokoye,

Abilez

2024

Cell Reports Methods

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Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs

Roeterink,

Casadevall i Solvas,

Collins

et al. 2024

Adv Healthcare Materials

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Mechanotransduction is the process whereby cells convert mechanical signals into electrochemical responses, where mechanosensitive proteins mediate this interaction. To characterize these critical proteins, numerous techniques have been developed that apply forces and measure the subsequent cellular responses. While these approaches have given insight into specific aspects of many such proteins, subsequent validation and cross‐comparison between techniques remain difficult given significant variations in reported activation thresholds and responses for the same protein across different studies. Accurately determining mechanosensitivity responses for various proteins, however, is essential for understanding mechanotransduction and potential physiological implications, including therapeutics. This critical review provides an assessment of current and emerging approaches used for mechanosensitive ion channel and G‐Coupled Receptors (GPCRs) stimulation and measurement, with a specific focus on the ability to quantitatively measure mechanosensitive responses.

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Piezo1 induces endothelial responses to shear stress via soluble adenylyl Cyclase-IP3R2 circuit

Cited by 8 publications

References 86 publications

Mechanosensitive channels in lung disease

Mechanosensitive channels in lung disease

Bioengineering methods for vascularizing organoids

Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs

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