PIEZO1 Ion Channels Mediate Mechanotransduction in Odontoblasts

Sun, Xiaohong; Qiao, Weiwei; Meng, Liuyan; Bian, Zhuan

doi:10.1016/j.joen.2022.02.005

Cited by 18 publications

(25 citation statements)

References 34 publications

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Section: Resultssupporting

confidence: 90%

“…Double immunofluorescent staining showed that most of the Piezo1+ pulpal axons (82.7 ± 1.6%) coexpressed NF200, and a few (2.9 ± 0.7%) coexpressed CGRP (Figures 3H,I). In addition, Piezo1 was expressed in odontoblasts (Figures 3F,G), consistent with recent electrophysiological and immunohistochemical findings of Piezo1 in rat odontoblasts and human odontoblast-like cells (Sato et al, 2018;Sun et al, 2022). At the EM level, the electron-dense immunostaining for Piezo1 was observed in the axoplasm of pulpal axons; in the peripheral pulp, the immunoreaction product was near the axonal plasma membrane of unmyelinated axons, possibly visualizing a membrane-bound pool of the receptor (Figures 4A-C).…”

Section: Axons In the Dental Pulp That Express Piezo1supporting

confidence: 90%

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Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp

Cho

Han

Jeong

et al. 2022

Front. Cell. Neurosci.

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Information on the neurons and axons that express the mechanosensitive channel Piezo1 and its expression in axons innervating the dental pulp may help understand the nature of the Piezo1-mediated mechanosensation and the underlying mechanism of dentin sensitivity elicited by mechanical stimuli. For this, we here investigated the neurochemical properties of the neurons in the rat trigeminal ganglion (TG) and their axons in its sensory root that express Piezo1 and the expression of Piezo1 in the rat and human dental pulp by light and electron microscopic immunohistochemistry and quantitative analysis. Piezo1 was expressed mainly in medium-sized and large TG neurons. Piezo1-immunopositive (+) neurons frequently coexpressed the marker for neurons with myelinated axons, NF200, but rarely the markers for neurons with unmyelinated axons, CGRP or IB4. In the sensory root of TG, Piezo1 was expressed primarily in small myelinated axons (Aδ, 60.2%) but also in large myelinated (Aβ, 24.3%) and unmyelinated (C, 15.5%) axons. In the human dental pulp, Piezo1 was expressed in numerous NF200+ axons, which formed a network in the peripheral pulp and often “ascended” toward the dentin. Most Piezo1+ myelinated axons in the radicular pulp became unmyelinated in the peripheral pulp, where Piezo1 immunoreaction product was associated with the axonal plasma membrane, suggesting a functional role of Piezo1 in the peripheral pulp. These findings suggest that Piezo1 is involved primarily in mediating the acute pain elicited by high-threshold mechanical stimuli, and that the Piezo1-mediated dental mechanotransduction occurs primarily in the axons in the peripheral pulp.

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Section: Resultssupporting

confidence: 90%

Section: Axons In the Dental Pulp That Express Piezo1supporting

confidence: 90%

Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp

Cho

Han

Jeong

et al. 2022

Front. Cell. Neurosci.

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“…The two methods used to evaluate TRPV1 activation through the measurement of calcium probe fluorescence showed similar patterns; however, the F/F0 ratio obtained from FC was higher than that obtained from the microplate reader. Fluo-4-AM has been widely used to measure calcium influx using fluorescence intensity 28 ; however, few studies have reported the use of cytofluorometry to measure calcium fluxes. 29 Wu et al, described that in Fluo-4 labelled cells, the ionomycin stimulus allows free entry of Ca 2+ , showing that this fluorophore allows the study of the activity of some ion channels and G-protein coupled receptors (GPCRs), using confocal microscopy and FC.…”

Section: Discussionmentioning

confidence: 99%

Capsazepine antagonizes TRPV1 activation induced by thermal and osmotic stimuli in human odontoblast-like cells

Cepeda

Velandia-Romero

Castellanos

2023

Journal of Oral Biology and Craniofacial Research

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Section: Piezo Channels In the Growth And Development Of Cranial Bonementioning

confidence: 99%

“…Odontoblasts express Piezo1 and Piezo2 channels, and their roles as mechanosensitive transducer cells in the dental sensory system have been widely explored (Khatibi Shahidi et al, 2015;Sato et al, 2018;Miyazaki et al, 2019;Lee et al, 2020;Matsunaga et al, 2021;Cho et al, 2022;Sun et al, 2022). Sun et al (2022) reported that Piezo1 is expressed in odontoblastic bodies and processes. Piezo1 mediates mechanical transduction in odontoblasts to release adenosine triphosphate (ATP), which transmits external signals to trigeminal ganglion (TG) neurons and generates pain responses.…”

Section: Piezo Channels In the Growth And Development Of Cranial Bonementioning

confidence: 99%

Mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial development and dentistry: Recent advances and prospects

2022

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Mechanical forces play important roles in many biological processes and there is increasing interest and understanding of these roles. Mechanotransduction is the process by which mechanical stimuli are converted to biochemical signals through specific mechanisms, and this results in the activation of downstream signaling pathways with specific effects on cell behaviors. This review systematically summarizes the current understanding of the mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial bone, tooth, and periodontal tissue, presenting the latest relevant evidence with implications for potential treatments and managements of dental and orofacial diseases and deformities. The mechanosensitive ion channels Piezo1 and Piezo2 are widely expressed in various cells and tissues and have essential functions in mechanosensation and mechanotransduction. These channels play an active role in many physiological and pathological processes, such as growth and development, mechano-stimulated bone homeostasis and the mediation of inflammatory responses. Emerging evidence indicates the expression of Piezo1 and Piezo2 in bone, dental tissues and dental tissue-derived stem cells and suggests that they function in dental sensation transduction, dentin mineralization and periodontal bone remodeling and modulate orthodontic tooth movement.

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PIEZO1 Ion Channels Mediate Mechanotransduction in Odontoblasts

Cited by 18 publications

References 34 publications

Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp

Expression of Piezo1 in the Trigeminal Neurons and in the Axons That Innervate the Dental Pulp

Capsazepine antagonizes TRPV1 activation induced by thermal and osmotic stimuli in human odontoblast-like cells

Mechanosensitive Piezo1 and Piezo2 ion channels in craniofacial development and dentistry: Recent advances and prospects

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