Piezoelectric Cell Growth Sensor

Ebersole, Richard C.; Foss, R. P.; Ward, Michael D.

doi:10.1038/nbt0591-450

Cited by 47 publications

(32 citation statements)

References 13 publications

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“…The decay of the signal may be influenced by adhesion of bacteria to the surface. However, the frequency shift is related to mass loading on the crystal surface, but when monitoring is done in liquid, the frequency also depends on the viscosity and density of the liquid in contact with the resonator (25,40) and is not necessarily directly proportional to the change in ƒ (3,7,11,19,29,37). The frequency shift is proportional to the attached mass only when the mass is rigid and tightly coupled to the surface (20), which is not the case for bacterial cells.…”

Section: Resultsmentioning

confidence: 99%

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Olofsson

Hermansson

Elwing

2005

Appl Environ Microbiol

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The reduction of bacterial biofilm formation on stainless steel surfaces by N-acetyl-L-cysteine (NAC) is attributed to effects on bacterial growth and polysaccharide production, as well as an increase in the wettability of steel surfaces. In this report, we show that NAC-coated stainless steel and polystyrene surfaces affect both the initial adhesion of Bacillus cereus and Bacillus subtilis and the viscoelastic properties of the interaction between the adhered bacteria and the surface. A quartz crystal microbalance with dissipation was shown to be a powerful and sensitive technique for investigating changes in the applied NAC coating for initial cell surface interactions of bacteria. The kinetics of frequency and dissipation shifts were dependent on the bacteria, the life cycle stage of the bacteria, and the surface. We found that exponentially grown cells gave rise to a positive frequency shift as long as their cell surface hydrophobicity was zero. Furthermore, when the characteristics of binding between the cell and the surface for different growth phases were compared, the rigidity increased from exponentially grown cells to starved cells. There was a trend in which an increase in the viscoelastic properties of the interaction, caused by the NAC coating on stainless steel, resulted in a reduction in irreversibly adhered cells. Interestingly, for B. cereus that adhered to polystyrene, the viscoelastic properties decreased, while there was a reduction in adhered cells, regardless of the life cycle stage. Altogether, NAC coating on surfaces was often effective and could both decrease the initial adhesion and increase the detachment of adhered cells and spores. The most effective reduction was found for B. cereus spores, for which the decrease was caused by a combination of these two parameters.Bacteria commonly adhere to a surface and are part of a complex biofilm. Since the growth of biofilms causes enormous economic losses and health problems in industrial systems like food and paper production, huge efforts are made to solve this problem. The adhesion of bacteria to surfaces is a complex interplay of physical, chemical, and biological factors (5), and there are large variations in the adhesion behaviors of different bacteria and also for different life cycle stages of one strain (41). It is very important to consider these differences when antiadhesion coatings are developed. It is generally thought that bacterial adhesion is preceded by the adsorption of a conditioning layer of molecules that influence adhesion of bacteria to surfaces (43). A good antiadhesion coating should form a conditioning film that prevents adhesion and has a low cohesive strength, making a weak bond between the biofilm and the surface (6). One such agent may be N-acetyl-L-cysteine (NAC). NAC is commonly used in medical treatment of chronic bronchitis and cancer (38,46) and is one of the smallest drug molecules in use (30). Moreover, we recently demonstrated that NAC affects several processes that are important for bacterial biofilm fo...

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Section: Resultsmentioning

confidence: 99%

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Olofsson

Hermansson

Elwing

2005

Appl Environ Microbiol

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“…Many sensors can be used to detect surface mass changes resulted from the incorporation of biological and chemical molecules on a sensitive surface area. Although the optical systems have been presented as the most advanced sensor system, piezoelectric devices represent a similar but significantly less expensive alternative [108][109][110].…”

Section: Conventional Sensorsmentioning

confidence: 99%

Relaxor-PT Single Crystal Piezoelectric Sensors

Jiang

Kim

2014

Crystals

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Relaxor-PbTiO 3 piezoelectric single crystals have been widely used in a broad range of electromechanical devices, including piezoelectric sensors, actuators, and transducers. This paper reviews the unique properties of these single crystals for piezoelectric sensors. Design, fabrication and characterization of various relaxor-PT single crystal piezoelectric sensors and their applications are presented and compared with their piezoelectric ceramic counterparts. Newly applicable fields and future trends of relaxor-PT sensors are also suggested in this review paper.

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“…For example, an easy determination of cell response to cell-affecting reagents is important in treatment of infectious diseases, or screening of new drugs and chemicals for their toxic effects. Ebersole et al (1991) suggest and anticipate that the piezoelectric sensor can be directly applied to the detection and characterization of eurokyotic cell growth. Previously reported piezoelectric methods for cell detection require selectivity, mass transduction, based on frequency-sensitive piezoelectric transduction and the quartz crystal resonator is (*), 20,000 ( ), 50,000 (&), 100,000 ( ), and 500,000 ( ) cell/gold sensor.…”

Section: Discussionmentioning

confidence: 99%

An alternative quantitative acoustical and electrical method for detection of cell adhesion process in real‐time

Guillou-Buffello

Gindre

Johnson

et al. 2010

Biotech & Bioengineering

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Sauerbrey [(1956), Z Phys 55:206-222] showed that the shift in resonance frequency of thickness shear mode (TSM) of a quartz crystal sensor is proportional to the mass, which is deposited on it. However, new powerful electrical circuits were developed that are capable of operating TSM quartz crystal sensors in fluids which enabled this method to be introduced into electrochemical and biological applications. These applications include the detection of virus capsids, bacteria, mammalian cells, the interaction of DNA and RNA with complementary strands, specific recognition of protein ligands by immobilized receptors, and last but not least the study of complete immunosensors. Piezoelectric quartz transducers allow a label-free identification of molecules; they are more than mass sensors since the biosensor response is also influenced by the surface charge of adsorbed proteins, interfacial phenomena, surface roughness and viscoelastic properties of the adhered biomaterial. These new characteristics have recently been used to investigate cell, liposome, and protein adhesion onto surfaces, thus permitting the rapid determination of morphological cell changes as a response to pharmacological substances, and changes in the water content of biopolymers avoiding of time-consuming methods. We validated an alternative quantitative acoustical engineering for cell adhesion process monitored by the TSM. Shear acoustical results (motional resistance) are further correlated to cell counting procedures and are sensitive of adhesion processes in real-time.

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Piezoelectric Cell Growth Sensor

Cited by 47 publications

References 13 publications

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Relaxor-PT Single Crystal Piezoelectric Sensors

An alternative quantitative acoustical and electrical method for detection of cell adhesion process in real‐time

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