Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis

Abstract

Up to now, there has been no report on target molecules detection by a piezoresistive microcantilever aptasensor. In order to evaluate the test performance and investigate the response dynamic characteristics of a piezoresistive microcantilever aptasensor, a novel method for ricin detection and kinetic analysis based on a piezoresistive microcantilever aptasensor was proposed, where ricin aptamer was immobilised on the microcantilever surface by biotin-avidin binding system. Results showed that the detection limit of ricin was 0.04μg L−1 (S/N ≥ 3). A linear relationship between the response voltage and the concentration of ricin in the range of 0.2μg L−1-40μg L−1 was obtained, with the linear regression equation of ΔUe = 0.904C + 5.852 (n = 5, R = 0.991, p < 0.001). The sensor showed no response for abrin, BSA, and could overcome the influence of complex environmental disruptors, indicating high specificity and good selectivity. Recovery and reproducibility in the result of simulated samples (simulated water, soil, and flour sample) determination met the analysis requirements, which was 90.5∼95.5% and 7.85%∼9.39%, respectively. On this basis, a reaction kinetic model based on ligand-receptor binding and the relationship with response voltage was established. The model could well reflect the dynamic response of the sensor. The correlation coefficient (R) was greater than or equal to 0.9456 (p < 0.001). Response voltage (ΔUe) and response time (t0) obtained from the fitting equation on different concentrations of ricin fitted well with the measured values.

A Genosensor Based on the Modification of a Microcantilever: A Review

“…The system showed a detection limit of 4 nM, and the study results also demonstrated that the RNA aptamer can bind to the siderophore binding pocket of the protein. In 2015, Liu et al [ 158 ] modified a dynamic microcantilever with the biotin–antibiotin system to detect ricin protein. In 2018, the group of Agarwal [ 159 ] detected a heart-type fatty acid-binding protein (h-FABP) in a trace amount (100 ng/mL) by employing a piezoresistive SU-8/CB microcantilever platform for the first time.…”

A Low Spring Constant Piezoresistive Microcantilever for Biological Reagent Detection

“…The relationship between the microcantilever response time and the ricin concentration is shown in Figure 9 c. The response time from injection to dynamic equilibrium were 2645 s, 3326 s, 4121 s, 4452 3, and 4875 s for the concentrations of ricin 10 ng/mL, 20 ng/mL, 40 ng/mL, 60 ng/mL and 100 ng/mL, respectively. The limiting factor for response time depend on a complicated dynamic process of the ricin aptamer and ricin [ 33 ]. At the same time, abrin, which is also a dimeric glycoprotein, was used as the target molecule to evaluate the specificity of the ricin aptamer sensor.…”

Preface: Micro-Nano Technology

“…For the Bio MEMS and Applications, the investigations of composite vascular repair grafts via Micro-imprinting and electrospinning 22 and piezoresistive Micro cantilever aptasensor for ricin detection and kinetic analysis are reported. 23 Also, the design of a telemetry system based on wireless power transmission for physiological parameters monitoring is proposed. 24 Three papers are shown in Nano materials field, two of which are focused on diamond film: voltammetric and impedance behaviors of surface-treated Nano-crystalline diamond film electrodes 25 and the effects of hydrogen atoms on surface conductivity of diamond film.…”