PIF4 and PIF4-Interacting Proteins: At the Nexus of Plant Light, Temperature and Hormone Signal Integrations

Xu, Yang; Zhu, Ziqiang

doi:10.3390/ijms221910304

Cited by 29 publications

(15 citation statements)

References 140 publications

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“…The TF WRKY34 interacts with the VQ20 protein to regulate pollen development and function 39 . The bHLH family TF PIF4 is necessary for plants to adapt to light and high ambient temperature, and interaction between PIF4 and PIF4 interacting proteins establishes a strong network for plants to respond to changes in the environment 40 . In addition, phytochrome interaction factors PIF4 and PIF5 negatively regulate anthocyanin synthesis in Arabidopsis seedlings under red light 41 .…”

Section: Discussionmentioning

confidence: 99%

Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza

Yang

2022

Sci Rep

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Flavonoid secondary metabolites can treat and prevent many diseases, but systematic studies on regulation of the biosynthesis of such metabolites in aboveground parts of Salvia miltiorrhiza are lacking. In this study, metabonomic and transcriptomic analyses of different S. miltiorrhiza phenotypes were conducted to explore pathways of synthesis, catalysis, accumulation, and transport of the main flavonoid secondary metabolites regulating pigment accumulation. Tissue localization and quantitative analysis of flavonoid secondary metabolites were conducted by laser scanning confocal microscopy (LSCM). A total 3090 differentially expressed genes were obtained from 114,431 full-length unigenes in purple and green phenotypes, and 108 functional genes were involved in flavonoid biosynthesis. Five key phenylpropane structural genes (PAL, 4CL, ANS, 3AT, HCT) were highly differentially expressed, and four transcription factor genes (MYB, WRKY, bHLH, bZiP) were identified. In addition, six GST genes, nine ABC transporters, 22 MATE genes, and three SNARE genes were detected with key roles in flavonoid transport. According to LSCM, flavonoids were mainly distributed in epidermis, cortex, and collenchyma. Thus, comprehensive and systematic analyses were used to determine biosynthesis, accumulation, and transport of flavonoids in stems and leaves of different S. miltiorrhiza phenotypes. The findings will provide a reference for flavonoid production and cultivar selection.

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Section: Discussionmentioning

confidence: 99%

Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza

Yang

2022

Sci Rep

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“…Until now, there has been little information on PIFs in crops. In our study, we found that the sequence of PIF4 protein was distinct from other PIFs homologs in rice, and it shows decreased gene expression followed by HL exposure from dark (Figure 3B-C), suggesting like PIF1, activated phyB interacts with PIF4 and induces its phosphorylation and degradation under light irradiation (Xu & Zhu, 2021).…”

Section: Discussionmentioning

confidence: 75%

PIF4 promotes water use efficiency during fluctuating light and drought resistance in rice

Liu

Essemine²,

Liu

et al. 2023

Preprint

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Molecular mechanism of intrinsic water use efficiency (iWUE) during fluctuating light (FL) was rarely understood. In this study, we investigated five parameters of iWUE under FL in 200 Minicore rice accessions. Among them, a novel trait, WUEFL(averaged iWUE during FL) has highest SNP heritability in these parameters. GWAS identifies six candidate genes, andPIF4is highly expressed in high iWUEFLrice subgroup. Nine SNPs were significantly associated with iWUEFL, and v3 SNP located at -1,075 bp ofPIF4promoter shows highest sensitives to light. Deletion of v3 in a rice cultivar, WYG7 (PIF4v3m) leads to ∼20% reduction in iWUEFL, and overexpressing PIF4 causes 25% increase in iWUEFLunder DS. There are 85% reduction in adenosine 3’,5’-diphosphate (PAP) amounts together with 73% increase inSAL1gene abundance in PIF4v3mthan WYG7. PIF4 transcriptionally repress and activateSAL1andNHX1, respectively, through binding to G-box motifs of the two genes, which leads to 16% reduction and 5% increase in iWUEFLin co-overexpression rice lines ofPIF4-SAL1andPIF4-NHX1, respectively, relative toPIF4-OE under DS. We proposed that PIF4 promotes iWUEFLand stomatal adjustment via targeting the G-box motif ofSAL1andNHX1genes during FL, eventually facilitating to drought resistance.

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“…The transcription of PIF4 is induced by high ambient temperatures [ 40 ], thereby enhancing the accumulation of PIF4 protein. The increased PIF4 protein induces hypocotyl growth by activating the expression of auxin biosynthetic genes such as YUCCA8 (YUC8) , CYP79B2, and TAA1 ; auxin signaling genes such as IAA19 and IAA29 ; growth-promoting genes such as ATHB2 and LNGs ; and brassinosteroid biosynthetic genes such as BES1 and BZR1 [ 41 ]. The evening complex (EC), which consists of ELF3 , ELF4 , and LUX , mediates the warm-temperature activation of PIF4 mRNA expression [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, previous studies have established that PIF4 is a core hub of thermomorphogenesis when plants are exposed to high ambient temperatures [ 40 , 41 , 46 , 47 , 48 , 49 , 50 ]. However, whether PIF4 also regulates HSR when plants are subjected to severe HS and the mechanistic details underlying this proceeding remains unclear.…”

Section: Introductionmentioning

confidence: 99%

PIF4 Promotes Expression of HSFA2 to Enhance Basal Thermotolerance in Arabidopsis

Yang

et al. 2022

IJMS

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Heat stress (HS) seriously restricts the growth and development of plants. When plants are exposed to extreme high temperature, the heat stress response (HSR) is activated to enable plants to survive. Sessile plants have evolved multiple strategies to sense and cope with HS. Previous studies have established that PHYTOCHROME INTERACTING FACTOR 4 (PIF4) acts as a key component in thermomorphogenesis; however, whether PIF4 regulates plant thermotolerance and the molecular mechanism linking this light transcriptional factor and HSR remain unclear. Here, we show that the overexpression of PIF4 indeed provides plants with a stronger basal thermotolerance and greatly improves the survival ability of Arabidopsis under severe HS. Via phylogenetic analysis, we identified two sets (six) of PIF4 homologs in wheat, and the expression patterns of the PIF4 homologs were conservatively induced by heat treatment in both wheat and Arabidopsis. Furthermore, the PIF4 protein was accumulated under heat stress and had an identical expression level. Additionally, we found that the core regulator of HSR, HEAT SHOCK TRANSCRIPTION FACTOR A2 (HSFA2), was highly responsive to light and heat. Followed by promoter analysis and ChIP-qPCR, we further found that PIF4 can bind directly to the G-box motifs of the HSFA2 promoter. Via effector–reporter assays, we found that PIF4 binding could activate HSFA2 gene expression, thereby resulting in the activation of other HS-inducible genes, such as heat shock proteins. Finally, the overexpression of PIF4 led to a stronger basal thermotolerance under non-heat-treatment conditions, thereby resulting in an enhanced tolerance to severe heat stress. Taken together, our findings propose that PIF4 is linked to heat stress signaling by directly binding to the HSFA2 promoter and triggering the HSR at normal temperature conditions to promote the basal thermotolerance. These functions of PIF4 provide a candidate direction for breeding heat-resistant crop cultivars.

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PIF4 and PIF4-Interacting Proteins: At the Nexus of Plant Light, Temperature and Hormone Signal Integrations

Cited by 29 publications

References 140 publications

Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza

Biosynthetic regulatory network of flavonoid metabolites in stems and leaves of Salvia miltiorrhiza

PIF4 promotes water use efficiency during fluctuating light and drought resistance in rice

PIF4 Promotes Expression of HSFA2 to Enhance Basal Thermotolerance in Arabidopsis

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