PiFlow: A Biocompatible Low-Cost Programmable Dynamic Flow Pumping System Utilizing a Raspberry Pi Zero and Commercial Piezoelectric Pumps

Kassis, Timothy; Perez, Paola M.; Yang, Chloe J. W.; Soenksen, Luis R.; Trumper, David L.; Griffith, Linda G.

doi:10.1101/192047

Cited by 4 publications

(4 citation statements)

References 70 publications

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“…In order to actively control fluid height within the hydrostatic fluid chamber, two Bartels Microtechnik mp6 piezoelectric pumps (Servoflo, Lexington, USA) are driven in a bidirectional configuration by the pumping board using two commercially available mp6-OEM driver circuits (Servoflo, Lexington, USA). 42 The flowrate of these piezoelectric pumps is controlled using a pulsewidth modulated (PWM) signal generated by two independent output channels from the HUZZAH® ESP8266 Feather. This type of pump was selected due to its small size, fast response, high dynamic range, and chemical inertness.…”

Section: Iia Feedback-controlled Gravity-driven Pump Setupmentioning

confidence: 99%

Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing

et al. 2018

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Precise fluid height sensing in open-channel microfluidics has long been a desirable feature for a wide range of applications. However, performing accurate measurements of the fluid level in small-scale reservoirs (<1 mL) has proven to be an elusive goal, especially if direct fluid-sensor contact needs to be avoided. In particular, gravity-driven systems used in several microfluidic applications to establish pressure gradients and impose flow remain open-loop and largely unmonitored due to these sensing limitations. Here we present an optimized self-shielded coplanar capacitive sensor design and automated control system to provide submillimeter fluid-height resolution (∼250 μm) and control of small-scale open reservoirs without the need for direct fluid contact. Results from testing and validation of our optimized sensor and system also suggest that accurate fluid height information can be used to robustly characterize, calibrate and dynamically control a range of microfluidic systems with complex pumping mechanisms, even in cell culture conditions. Capacitive sensing technology provides a scalable and cost-effective way to enable continuous monitoring and closed-loop feedback control of fluid volumes in small-scale gravity-dominated wells in a variety of microfluidic applications.

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Section: Iia Feedback-controlled Gravity-driven Pump Setupmentioning

confidence: 99%

Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing

et al. 2018

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show abstract

“…In order to actively control fluid height within the hydrostatic fluid chamber, two Bartels Microtechnik mp6 piezoelectric pumps (Servoflo, Lexington, USA) are driven in a bidirectional configuration by the pumping board using two commercially available mp6-OEM driver circuits (Servoflo, Lexington, USA). 33 The flow-rate of these piezoelectric pumps is controlled using a pulse-width modulated (PWM) signal generated by two independent output channels from the HUZZAH® ESP8266 Feather. This type of pump was selected due to its small size, fast response, high dynamic range, and chemical inertness.…”

Section: Iia4 Bidirectional Supply Pumpsmentioning

confidence: 99%

Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing

Soenksen

Kassis

Griffith

et al. 2017

Preprint

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“…The rapid development of micro uidic technology is a way of mimicking an in vivo-like microenvironment [33][34][35][36] . The primary purpose of producing microstructures is to convert today's complicated and costly laboratories into fast, inexpensive, and high-e cient micro-scale laboratories 37,38 .…”

Section: Introductionmentioning

confidence: 99%

Cell-imprinted-based integrated microfluidic device for biomedical applications: Computational and experimental studies

Kashani

Moraveji

Bonakdar

2021

Preprint

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It has been proved that cell-imprinted substrates molded from template cells can be used for the re-culture of that cell while preserving its normal behavior or to differentiate the cultured stem cells into the template cell. In this study, a microfluidic device was presented to modify the previous irregular cell-imprinted substrate and increase imprinting efficiency by regular and objective cell culture. First, a cell-imprinted substrate from template cells was prepared using a microfluidic chip in a regular pattern. Another microfluidic chip with the same pattern was then aligned on the cell-imprinted substrate to create a chondrocyte-imprinted-based integrated microfluidic device. Computational fluid dynamics (CFD) simulations were used to obtain suitable conditions for injecting cells into the microfluidic chip before performing experimental evaluations. In this simulation, the effect of input flow rate, number per unit volume, and size of injected cells in two different sizes of the chip were examined on exerted shear stress and cell trajectories. This numerical simulation was first validated with experiments with cell lines. Finally, chondrocyte was used as template cell to evaluate the chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADSCs) in the chondrocyte-imprinted-based integrated microfluidic device. ADSCs were positioned precisely on the chondrocyte patterns, and without using any chemical growth factor, their fibroblast-like morphology was modified to the spherical morphology of chondrocytes after 14 days of culture. Both immunostaining and gene expression analysis showed improvement in chondrogenic differentiation compared to traditional imprinting methods. This study demonstrated the effectiveness of the cell-imprinted-based integrated microfluidic devices for biomedical applications.

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PiFlow: A Biocompatible Low-Cost Programmable Dynamic Flow Pumping System Utilizing a Raspberry Pi Zero and Commercial Piezoelectric Pumps

Cited by 4 publications

References 70 publications

Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing

Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing

Closed-loop feedback control for microfluidic systems through automated capacitive fluid height sensing

Cell-imprinted-based integrated microfluidic device for biomedical applications: Computational and experimental studies

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