Pig reticulocytes. III. Glucose permeability in naturally occurring reticulocytes and red cells from newborn piglets.

Kim, H D; Luthra, Madan G.

doi:10.1085/jgp.70.2.171

Cited by 26 publications

(11 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The decrease in membrane permeability to glucose seen in reticulocytes undergoing maturation confirms and corroborates the results suggested by the earlier work of Kim and Luthra (1977). In the newborn pig, the reticulocytes present at birth still possess a small but well-defined permeability to glucose.…”

Section: Discussionsupporting

confidence: 89%

“…However, an impressive latent glycolytic capacity can be demonstrated either in the adult pig cells rendered pervious to glucose by treatment with a polene antibiotic, amphotericin B (Kim and McMannus, 1971 a,b) or in the postnatal cells which have a high glucose permeability, exhibiting carrier-mediated transport (Kim et al, 1971;Zeidler et al, 1976). Moreover, a transitory glucose permeability with a concomitant glycolysis takes place both in the experimentally induced reticulocytes of the adult animal (Kim et al, 1976 a,b) and in the naturally occurring reticulocytes of the postnatal piglets (Kim and Luthra, 1977). The results emerging from this laboratory indicated that the membrane permeability to glucose is quickly lost in the course of normal maturation of reticulocytes to erythrocytes in vivo (Kim et al, 1976 a,b;Kim and Luthra, 1977).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Pig reticulocytes. IV. In vitro maturation of naturally occurring reticulocytes with permeability loss to glucose

Zeidler

Kim

1982

Journal Cellular Physiology

View full text Add to dashboard Cite

Naturally occurring reticulocytes of week old piglets were used to characterize the maturation process under in vitro conditions. When the reticulocytes were suspended in tissue culture medium fortified with metabolic substrates, nearly all cells were viable after 24 hours incubation and usually more than 85% of the initial cell population survived after an 80 hour period. In cells maintained as long as a week in incubation, an adequate level of total adenine nucleotide with a large accumulation of IMP was found. In most cases, reticulocytes lose their reticular materials within two days and assume normal erythrocyte configuration. Concomitant with the morphological change, the cell volume decreases toward normal erythrocyte size, the extent of which can be accounted for by the intracellular loss of salt and accompanying water. As in the in vivo reticulocyte maturation process, reticulocytes undergoing in vitro maturation lose their membrane permeability to glucose. These findings suggest that the process of reticulocyte maturation occurring in cell culture approaches that which naturally occurs in vivo. Thus, these cells may be used to delineate the mechanism of the loss of membrane transport of glucose which normally occurs in the adult pig cells.

show abstract

Section: Discussionsupporting

confidence: 89%

mentioning

confidence: 99%

Pig reticulocytes. IV. In vitro maturation of naturally occurring reticulocytes with permeability loss to glucose

Zeidler

Kim

1982

Journal Cellular Physiology

View full text Add to dashboard Cite

show abstract

“…Kim & Luthra (1977) using density separation and 5l0r and 59Fe labelling techniques suggested that the loss of transport activity in the pig after birth resulted from the replacement of glucose-permeable cells with glucose-impermeable erythrocytes. Unlike the pig, the sheep possesses a distinct fetal haemoglobin, and in the present series of experiments it was possible to compare directly the changes in the two parameters.…”

Section: Discussionmentioning

confidence: 99%

Nucleoside and glucose transport in erythrocytes from new‐born lambs

Mooney¹,

Young²

1978

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. Glucose and inosine transport by erythrocytes from new-born lambs and adult sheep were compared. Uptake of both permeants was considerably faster in the new-born. Inosine uptake by erythrocytes from nucleoside-permeable and impermeable lambs were not significantly different at birth. The difference between the two phenotypes was first apparent 30 days after birth.2. The post-natal changes in glucose and inosine transport activity closely paralleled the progressive decrease in the percentage of fetal erythrocytes (i.e. cells containing fetal haemoglobin) in the circulation. Cell fractionation studies confirmed that the permeability changes were directly related to changes in the relative proportions of fetal and adult haemoglobin containing erythrocytes.3. The results demonstrate that fetal cells are highly permeable to both glucose and inosine. These cells are replaced by erythrocytes which contain adult haemoglobin and which have a much lower, but still significant, glucose permeability and either low or negligible inosine transport activity depending on the genotype of the animal.4. Inosine transport by fetal erythrocytes from both nucleoside-permeable and impermeable animals was mediated by a nucleoside transport system which had similar properties to that responsible for nucleoside transport in adult nucleosidepermeable cells. Glucose transport in both fetal and adult cells was highly stereospecific, indicating the presence of a selective transport system. 5. It is suggested that the regulatory mechanism responsible for initiating the switch from fetal to adult haemoglobin synthesis may also be responsible for the changes in glucose and nucleoside transport activity.

show abstract

“…The reticulocytes were stained with new methylene blue dye (Sigma Chemical Co., St. Louis, Mo.) as described elsewhere (Kim and Luthra, 1977).…”

Section: Anemic Stressmentioning

confidence: 99%

LK sheep reticulocytosis: effect of anti-L on K influx and in vitro maturation.

Kim¹,

Theg²,

Lauf³

1980

The Journal of General Physiology

Self Cite

View full text Add to dashboard Cite

After massive hemorrhage, adult sheep with genotypically low potassium (LK) red cells temporarily produce high potassium (HK) cells with ouabain-sensitive K + pump fluxes equivalent to mature HK red cells. In light of recent reports of different red cell volume populations accompanying the HK-LK transition also occurring in newborn LK sheep and the unresolved controversy over the effect of anti-L on K + transport in these immature red cells, we have reexamined the K + transport changes and the effect of anti-L in the newly formed HK cells at various times after anemic stress and under in vitro conditons. We found that ~7 d after bleeding, maximum reticulocytosis occurred in the peripheral blood. After separation by density centrifugation, the top 10% cell fraction contained 100% reticulocytes, with a mean cell volume 2.5 times larger than that of mature erythrocytes. These immature red cells were of HK type, and their K + pump and leak fluxes were 30 and 10 times higher, respectively, than those found in mature LK cells. The new cells may possess HK-and LK-type pumps because K + pump influx was significantly stimulated by anti-L. When separated by density centrifugation on days 9, 17, and 23 after bleeding, some of the cells apparently maintained their large size while gaining higher density. Large cells from day 9, kept in vitro for 22 h, showed anti-Lsensitive K + pump and leak fluxes that declined within hours, paralleling the behavior of these cells in vivo, whereas cellular K + levels changed much less. It is concluded that the newly formed red cells may belong to a stress-induced macrocytic cell population that does not acquire all of the characteristics of adult LK cells.

show abstract

Pig reticulocytes. III. Glucose permeability in naturally occurring reticulocytes and red cells from newborn piglets.

Cited by 26 publications

References 15 publications

Pig reticulocytes. IV. In vitro maturation of naturally occurring reticulocytes with permeability loss to glucose

Pig reticulocytes. IV. In vitro maturation of naturally occurring reticulocytes with permeability loss to glucose

Nucleoside and glucose transport in erythrocytes from new‐born lambs

LK sheep reticulocytosis: effect of anti-L on K influx and in vitro maturation.

Contact Info

Product

Resources

About