Pigment containing lipid vesicles

Walz, D.

doi:10.1007/bf01869398

Cited by 11 publications

(3 citation statements)

References 33 publications

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“…a solvent which also carries an acyl ester group, and the similar EPC in the two systems. Our conclusion is in agreement with the views proposed by Oettmeier et al (1976) and Walz (1977) for the location of Chl a in vesicle membranes.…”

Section: Disclssioxsupporting

confidence: 94%

SITE‐SELECTION SPECTROSCOPY OF CHLOROPHYLL b IN MEMBRANES OF LECITHIN VESICLES AND IN OTHER SOLVENTS

Fünfschilling

Walz

1983

Photochem & Photobiology

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Abstract— Site‐selection fluorescence spectra of chlorophyll b in membranes of lecithin vesicles and in the solvents ethanol, n‐butanol, n‐butyl acetate, 2‐methyl tetrahydrofuran and toluene are presented. The spectra in vesicles display zero‐phonon lines as pronounced as those in the best organic glasses. The characteristics of the distributions of O—O transition energies and of the electron‐phonon couplings allow to infer the position of the chlorophyll molecule in the membrane of vesicles. It is thus found that the chromophore of chlorophyll b is situated in the layer formed by the ester moieties of the lecithins, i. e. close to the surface of the membrane but not in direct contact with the aqueous phase.

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Section: Disclssioxsupporting

confidence: 94%

SITE‐SELECTION SPECTROSCOPY OF CHLOROPHYLL b IN MEMBRANES OF LECITHIN VESICLES AND IN OTHER SOLVENTS

Fünfschilling

Walz

1983

Photochem & Photobiology

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“…Walz [29,30] suggests that the orientation of the chlorine ring may depend on the aggregational state of the lipid membrane (cf. also Ref.…”

Section: Discussionmentioning

confidence: 99%

Influence of lecithin liposomes on chlorophyllase-catalyzed chlorophyll hydrolysis. Comparison of intramembraneous and solubilized Phaeodactylum chlorophyllase

Terpstra¹

1980

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Summary1. Chlorophyllase-catalyzed chlorophyll hydrolysis is greatly enhanced by the addition of divalent cations (Mg 2÷) combined with a reducing agent (dithiothreitol, ascorbate). A similar effect is obtained by the addition of lecithin. In the presence of lecithin, dithiothreitol has only slight or no influence on chlorophyll hydrolysis. Mg 2÷ eliminates the activating effect of lecithin.2. In the absence of Mg 2÷ + dithiothreitol or of lecithin, Triton X-100 has a slight activating effect on chlorophyllase-catalyzed chlorophyll hydrolysis, but only at low concentrations (0.01--0.02%}. In the presence of Mg 2÷ and dithiothreitol or of lecithin, Triton X-100 (greater than or equal to 0.02%} inhibits this reaction.3. Whereas chlorophyllase combines with chlorophyll, no combination of chlorophyllase and lecithin could be detected.4. Solubilized chlorophyllase is stabilized by its substrate, chlorophyll. Enzyme stabilization is eliminated by lecithin, whereas in the absence of chlorophyll, denaturation is somewhat increased by dithiothreitol.5. No clear difference was found between the actions of intramembraneous and solubilized chlorophyllase. The results suggest that chlorophyllase is situated within membranes in such a way that the active group protrudes into the aqueous medium surrounding the membrane. 6. A hypothesis explaining the activating effects which Mg 2÷ combined with

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“…It was suggested that Chl molecules are located just below the membrane surface [213]. The spectroscopic and fluorescence data point to the polar environment of Chl tetrapyrrol macroring rather than to its direct contact with the aqueous phase [214,215]. Most likely this polar environment is formed by the domains of the ester groups of lecithin molecules.…”

Section: Chlorophyll-containing Membranesmentioning

confidence: 94%