Pigment epithelium-derived factor (PEDF) plays anti-inflammatory roles in the pathogenesis of dry eye disease

Ma, Baikai; Zhou, Yifan; Liu, Rongjun; Zhang, Kai; Yang, Tingting; Hu, Chenxi; Gao, Yufei; Lan, Qianqian; Liu, Yiyun; Xiao, Yang; Qi, Hong

doi:10.1016/j.jtos.2020.12.007

Cited by 55 publications

(36 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also beneficial for obtaining quick, efficient, reliable, and robust results in the early stages of candidate drug screening. In DED, some established in vitro models have also been established: the Wong Kilbourne derivative of Chang conjunctival epithelial cell line (WKD; clone 1-5c-4) [47][48][49][50][51]; immortalized normal human conjunctival cell line (IOBA-NHC) [47,52,53]; human corneal epithelial cells (HCECs), a human transformed SV40 immortalized corneal epithelial cell line [54,55], and human conjunctival cell line (HCC) [56]. In vitro models with primary cell culture are also available: human corneal epithelial cells (HCECs) [57], rabbit corneal epithelial cells (CECs), or rabbit LG acinar cells (LGACs) [58].…”

Section: In Vitro Dry Eye Modelmentioning

confidence: 99%

“…In vitro models with primary cell culture are also available: human corneal epithelial cells (HCECs) [57], rabbit corneal epithelial cells (CECs), or rabbit LG acinar cells (LGACs) [58]. The induction of DED-like symptoms in in vitro culture can be performed by the induction of hyperosmotic (500 mOsM) media in the culture media by the addition of 90 mM sodium chloride in the culture medium, which triggers inflammatory-like symptoms [47,48,55,57,59]. As in the in vivo experiment, benzalkonium chloride (BAC) (0.0001% (1 µg/mL) was also used to induce inflammatory cascades [47][48][49][50][51][52].…”

Section: In Vitro Dry Eye Modelmentioning

confidence: 99%

See 1 more Smart Citation

Experimental Models, Induction Protocols, and Measured Parameters in Dry Eye Disease: Focusing on Practical Implications for Experimental Research

Rahman

Kim

2021

IJMS

View full text Add to dashboard Cite

Dry eye disease (DED) is one of the major ophthalmological healthcare challenges worldwide. DED is a multifactorial disease characterized by a loss of homeostasis of the tear film, and its main pathogenesis is chronic ocular surface inflammation related with various cellular and molecular signaling cascades. The animal model is a reliable and effective tool for understanding the various pathological mechanisms and molecular cascades in DED. Considerable experimental research has focused on developing new strategies for the prevention and treatment of DED. Several experimental models of DED have been developed, and different animal species such as rats, mice, rabbits, dogs, and primates have been used for these models. Although the basic mechanisms of DED in animals are nearly identical to those in humans, proper knowledge about the induction of animal models is necessary to obtain better and more reliable results. Various experimental models (in vitro and in vivo DED models) were briefly discussed in this review, along with pathologic features, analytical approaches, and common measurements, which will help investigators to use the appropriate cell lines, animal, methods, and evaluation parameters depending on their study design.

show abstract

Section: In Vitro Dry Eye Modelmentioning

confidence: 99%

Section: In Vitro Dry Eye Modelmentioning

confidence: 99%

Experimental Models, Induction Protocols, and Measured Parameters in Dry Eye Disease: Focusing on Practical Implications for Experimental Research

Rahman

Kim

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Recently, Ma et al found that PEDF could inhibit the expression of inflammatory cytokines IL-1β, IL-6, TNF-α, and IL-17A in DED and the percentage of Th17 cells in vivo and in vitro experiments. It was also found that PEDF inhibited the phosphorylation of MAPK p38 and JNK in hypertonic CECs (Ma et al, 2021). All the above studies have shown that PEDF plays anti-inflammatory and immunoregulatory roles in the pathogenesis of DED.…”

Section: Immunoregulatory Molecules and Inflammationmentioning

confidence: 78%

Recent Developments About the Pathogenesis of Dry Eye Disease: Based on Immune Inflammatory Mechanisms

Dong

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Dry eye disease is a common and frequently occurring ophthalmology with complex and diverse causes, and its incidence is on the upward trend. The pathogenesis of DED is still completely clear. However, the immune response based on inflammation has been recognized as the core basis of this disease. In this review, we will systematically review the previous research on the treatment of DED in immune inflammation, analyze the latest views and research hotspots, and provide reference for the prevention and treatment of DED.

show abstract

“…PEDF is nontoxic [77]; 3. PEDF is multimodal and possesses neurotrophic [27], antiangiogenic [55], anti-inflammatory [78], antioxidant properties [79,80], all of which are beneficial for the treatment of several multifactorial retinal diseases; 4. PEDF is a soluble protein that can diffuse from the vitreous to the back of the eye and from the subconjuntiva to the retina [81][82][83][84]; 5.…”

Section: Discussionmentioning

confidence: 99%

Delivery Systems of Retinoprotective Proteins in the Retina

Rebustini¹,

Bernardo-Colón²,

Nalvarte³

et al. 2021

IJMS

View full text Add to dashboard Cite

Retinoprotective proteins play important roles for retinal tissue integrity. They can directly affect the function and the survival of photoreceptors, and/or indirectly target the retinal pigment epithelium (RPE) and endothelial cells that support these tissues. Retinoprotective proteins are used in basic, translational and in clinical studies to prevent and treat human retinal degenerative disorders. In this review, we provide an overview of proteins that protect the retina and focus on pigment epithelium-derived factor (PEDF), and its effects on photoreceptors, RPE cells, and endothelial cells. We also discuss delivery systems such as pharmacologic and genetic administration of proteins to achieve photoreceptor survival and retinal tissue integrity.

show abstract

Pigment epithelium-derived factor (PEDF) plays anti-inflammatory roles in the pathogenesis of dry eye disease

Cited by 55 publications

References 50 publications

Experimental Models, Induction Protocols, and Measured Parameters in Dry Eye Disease: Focusing on Practical Implications for Experimental Research

Experimental Models, Induction Protocols, and Measured Parameters in Dry Eye Disease: Focusing on Practical Implications for Experimental Research

Recent Developments About the Pathogenesis of Dry Eye Disease: Based on Immune Inflammatory Mechanisms

Delivery Systems of Retinoprotective Proteins in the Retina

Contact Info

Product

Resources

About