Pigmentary Changes in the Skin

Nj, Pelc; Jj, Nordlund

doi:10.1016/s0094-1298(20)30772-0

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…In Figure 1, the melanization of the clinical CSS is heterogeneous with multiple pigmented foci. Passenger HMs persist in the HK culture and lead to a range of pigment patterns from diffusely hyperpigmented to irregularly hypopigmented 4–6 . Hypopigmentation is most commonly seen in CSS grafts prepared from cryopreserved HKs because the passenger HMs do not survive the cryopreservation process well.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the success with CSS in treating burn wounds, CSS are deficient in many of the structures and functions of uninjured skin, such as sebaceous glands, melanocytes, hair follicles, endothelial cells, nerve cells and cell‐mediated immunity 3 . In postburn healing wounds, abnormal pigmentation patterns include both hyperpigmentation and hypopigmentation 4–6 . The skin dyschromia can take months to years to normalize.…”

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confidence: 99%

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Regulation of cutaneous pigmentation by titration of human melanocytes in cultured skin substitutes grafted to athymic mice

Swope

Supp²,

Boyce

2002

Wound Repair Regeneration

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Pigmentation of healed cultured skin substitutes in burn patients is frequently irregular and unpredictable which compromises solar protection and the patient's self-image. To address these morbidities, human fibroblasts were inoculated on a collagen-glycosaminoglycan substrate followed 1 day later by the addition of keratinocytes at 1.1 x 10(6)/cm2 combined with either 0, 1.1 x 10(2), 1.1 x 10(3), or 1.1 x 10(4) melanocytes/cm2. The skin substitutes were incubated in vitro for 3 weeks and grafted to athymic mice. In vitro, the number of L-Dopa-positive melanocytes in the skin substitutes increased proportionately to the number of melanocytes inoculated. The melanocytes localized to the basal epidermis when labeled for MEL-5. The skin substitutes with 1.1 x 10(4) melanocytes/cm2 were significantly darker than other groups in vitro by chromameter evaluation. By 12 weeks after grafting, the cultured skin ranged from no pigment in the control group, to 75% pigmented area in the 1.1 x 10(3) melanocytes/cm2 group, to complete pigmentation in the 1.1 x 10(4) melanocytes/cm2 group. In vivo, the mean chromameter values were significantly darker for the grafts with 1.1 x 10(3) and 1.1 x 10(4) melanocytes/cm2. These results suggest that complete restoration of cutaneous pigmentation can be accomplished by addition of between 0.1 and 1.0 x 10(4) melanocytes/cm2 to skin substitutes.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Regulation of cutaneous pigmentation by titration of human melanocytes in cultured skin substitutes grafted to athymic mice

Swope

Supp²,

Boyce

2002

Wound Repair Regeneration

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Electrophoto-Biomodulation in Aesthetic Treatment of Postburn Hypopigmentation

Elmelegy

Sakka

2017

Annals of Plastic Surgery

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Regulation of Pigmentation in Cultured Skin Substitutes by Cytometric Sorting of Melanocytes and Keratinocytes

Swope

Supp²,

Cornelius³

et al. 1997

Journal of Investigative Dermatology

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Unpredictable pigmentation in cultured skin substitutes (CSS) is an anatomic deficiency after wound treatment and can require years to normalize. Variable numbers of human melanocytes (HM) survive in cultures of human keratinocytes (HK) as demonstrated by focal areas of pigmentation in CSS after healing. The purposes of this study were to deplete HM from HK cultures and to regulate the numbers of HM contained in CSS. A highly pigmented HM cell strain was chosen for these studies to emphasize the differences in light scattering between HK and HM by flow cytometry. Cytometric gates were set with selective cultures of HM and HK and were used to sort a mixed population of HK + 4% HM. After sorting, CSS were prepared from human fibroblasts attached to collagen-glycosaminoglycan sponges combined with cells from the HK + 4% HM (pre-treatment control), the sorted HK (experimental), or sorted HK + 3% HM (post-treatment positive control) subpopulations and grafted to athymic mice. Grafted wounds were assessed for 6 wk by planimetry for area of pigment and by a Minolta Chromameter for color density and hue in situ. Histology and staining of HLA-ABC were performed at 6 wk. Data from percent pigmented area and chromameter measurements identified quantitative and statistically significant decreases in color of healed skin after flow cytometric separation of HK and HM. Therefore, a purified HK subpopulation depleted of HM was isolated by flow cytometry that generated healed skin with reduced pigmentation. These results suggest that HM can be selectively depleted from HK cultures and then added to cultured skin substitutes at specific densities to generate predictable pigmentation for improved function and cosmesis in healed wounds.

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Pigmentary Changes in the Skin

Cited by 3 publications

References 8 publications

Regulation of cutaneous pigmentation by titration of human melanocytes in cultured skin substitutes grafted to athymic mice

Regulation of cutaneous pigmentation by titration of human melanocytes in cultured skin substitutes grafted to athymic mice

Electrophoto-Biomodulation in Aesthetic Treatment of Postburn Hypopigmentation

Regulation of Pigmentation in Cultured Skin Substitutes by Cytometric Sorting of Melanocytes and Keratinocytes

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