Pigmented melanoma cell migration study on murine syngeneic B16F10 melanoma cells or tissue transplantation models

Abstract

Melanoma is a lethal form of skin cancer with poor prognosis, especially due to the early metastatic feature. Recent studies have shown that the melanin pigment influences the nanomechanical properties and, therefore, the metastatic behavior of the melanoma cells. We aimed to study the growth of subcutaneously transplanted syngeneic melanoma tissue in female C57BL/6 mice harvested from a mouse with a four-week B16F10 melanoma. Also, we studied the effect of the melanin pigment loading on the peritumoral migratory abilities of melanoma cells. Even when the syngeneic transplant was different (cultured cells vs. tumor tissue), the morphological features and the tumor growth were similar in both groups of mice. Heavily pigmented melanoma cells had low migration abilities. Angiogenesis, the depigmentation phenomenon, and the cell shape changes were related to pigmented melanoma cell migration along the matrix collagen fibers of peritumoral structures: the abluminal face of the vessels (angiotropism), the endomysium, and the nerves (neurotropism). The replacement of the histopathological growth pattern, the absence of angiogenesis, and rapidly tumor-bearing emboli were correlated with amelanotic and low pigmented melanoma cells. This study demonstrated that syngeneic melanoma tissue transplantation was a viable technique, and that the melanin pigment loading level can affect the melanoma cell migration profile.

Phenotypic Switching of B16F10 Melanoma Cells as a Stress Adaptation Response to Fe3O4/Salicylic Acid Nanoparticle Therapy

“…Both of the structural components of SaIONs can induce tumor cell stress by altering the activity of intracellular organs: the iron oxide cores alter the function of the mitochondria [ 40 ], while the salicylic acid shells alter the function of the endoplasmic reticulum [ 41 , 42 ]. In another study, some of us highlighted the cytotoxic effect of salicylic acid/iron oxide nanoparticles on melanoma B16F10 xenografts [ 64 ].…”

“…In this work, we performed a morphological and morphometric evaluation of the phenotypic changes and the pigmentation process induced in B16F10 murine melanoma following therapy with an aqueous dispersion of SaIONs. To achieve this, a syngeneic model of B16F10 tissue melanoma implanted in C57BL/6 mice was used, according to a previously reported technique [ 64 ]. The phenotypic heterogeneity and tumor pigmentation of the B16F10 murine melanomas were evaluated both in the absence of therapy (M2w and M4w groups sacrificed at two and four weeks, respectively) and after SaIONs therapy, performed by three administration schemes: oral administration (T2w and T4w groups treated for two and four weeks, respectively), intratumoral injection (ITM1w group), and combined administration, first orally, followed by an intratumoral injection (ITO1w group).…”

The Challenging Melanoma Landscape: From Early Drug Discovery to Clinical Approval

“…One of the most studied is the B16-F10 cell line, which is derived from melanoma induced in C57BL/6 mice [84][85][86]. These cells are highly metastatic and present strong pigmentation [87]. Other examples of murine melanoma cells are K1735-M2 and YUMM [88,89].…”