Pigments, Polyphenols and Antioxidant Activity of Leaf Extracts from Four Wild Rose Species Grown in Sicily

Abstract

Leaf methanolic extracts of four wild Rose species (Rosa canina L., R. corymbifera Borkh., R. micrantha Borrer ex Sm and R. sempervirens L.) grown in Sicily were analysed in order to evaluate differences among indigenous roses which could be used such as source of bioactive compounds and natural antioxidants. Leaves were harvested during two different periods (June and October) and their morphological characteristics were described. Leaf content of chlorophylls, total anthocyanins, total flavonoids, total polyphenols (Folin-Ciocalteu method) and antioxidant activity (DPPH method) were determined. Results showed that the contents of chlorophylls and carotenoids were higher in leaves of all species harvested in June than in those harvested in October. On the contrary, total anthocyanins of R. micrantha and R. corymbifera leaves were higher in October. Total polyphenol content was higher in leaves of all species were harvested in October. No difference in total flavonoids content was recorded between the two harvest periods but only among the four species. Antioxidant activity was generally high in leaf extracts of all species in both periods and, particularly, in R. micrantha leaves harvested in June. These outcomes seem to support the assessment that leaves harvested from some Sicilian wild roses may be used as promising source of healthy compounds and that their content varies with the species and harvest period.

LC‐DAD‐ESI‐MS and HPLC‐DAD phytochemical investigation and in vitro antioxidant assessment of Rosa sp. stem pruning products from different northern areas in Tunisia

“…According to Bitis et al ., R. sempervirens leaves showed considerable high radical‐scavenging effect (DPPH and TEAC) by their EtOAc extracts. D'angiolillo et al . found that the radical scavenging activity of Rosa leaf extracts as evaluated by DPPH test, was affected by species variation and harvest period.…”

“…found that the radical scavenging activity of Rosa leaf extracts as evaluated by DPPH test, was affected by species variation and harvest period. In this report, R. canina leaf extracts were compared to three other roses ( R. corymbifera , R. micrantha and R. sempervirens ) and demonstrated to exhibit the highest DPPH radical scavenging ability when harvested in June and the lowest power when collected in October. As reported in a recent study and in contrast to our results with stems, the antioxidant ability of rose hip extracts of R. sempervirens measured by ORAC assay, was significantly more important (4576.77 μmol TE/g DE) than that recorded for R. canina (4493.64 μmol TE/g DE), R. micrantha (3761.11 μmol TE/g DE) and R. corymbifera (2726.68 μmol TE/g DE).…”

Oxidative Stress Responses of Some Endemic Plants to High Altitudes by Intensifying Antioxidants and Secondary Metabolites Content

“…Moreover, there was a significant increase in the content of chlorophyll a, chlorophyll b, and carotenoids (Figure 1). Carotenoids, in addition to being active as light-harvesting pigments [55,56], act as a scavenger of singlet oxygen species and quench the triplet state of chlorophyll molecules [57]. Under abiotic stress, cellular homeostasis is disrupted, leading to the production of free radicals, which in turn can result in oxidative damage [58,59].…”

“…Moreover, there was a significant increase in the content of chlorophyll a, chlorophyll b, and carotenoids ( Figure 1 ). Carotenoids, in addition to being active as light-harvesting pigments [ 55 , 56 ], act as a scavenger of singlet oxygen species and quench the triplet state of chlorophyll molecules [ 57 ].…”

Assessment of endophytic bacterial diversity in rose by high-throughput sequencing analysis

“…At the same time, rose possesses cosmetic and skin-moisturizing properties, can help reduce pain, functions as an aromatic deodorant, and invigorates the spleen. Its medicinal value is extremely high [16][17][18]. It is therefore worthwhile to explore the diversity and function of rose endophytic bacteria because they affect the functional properties of the roses themselves.…”