Pili and the interaction of Aeromonas species with humanperipheral blood polymorphonuclear cells

Kamperman, L

doi:10.1016/0928-8244(93)90069-g

Cited by 2 publications

(4 citation statements)

References 20 publications

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“…Carbohydrate-reactive OMPs (CROMPS), which may be porins for fucose, have also been postulated as adhesins and may be intestinal adhesive factors [27,28], but do not appear to mediate binding to cell lines. The A. hydrophila strain (strain A6) from which these OMPs were purified was not adhesive in the above cell line assays [3,16]. Indeed, we have found that most A. hydrophila strains, even those isolated from diarrhoeal faeces as sole potential pathogen (eg.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteria and preparations of filamentous structures obtained from bacterial strains by mechanical shearing were examined under a Philips 410 electron microscope at 80 kV following staining with uranyl acetate (1 %) for 30 s to 1 min as described elsewhere [16].…”

Section: Electron Microscopymentioning

confidence: 99%

“…* Aean +standard deviation of duplicate coxerslips from one or more experiments (n = number of times adhesion assay was performed). Bacteria were grown in TSBY at 37°C for [16][17][18] h, at 22 0(1 for 2 days and at 7°C for 7 days.…”

Section: Effect Of Rernoval Of Surface Structures On Adhesive Abilitymentioning

confidence: 99%

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Adhesion ofAeromonassp. to cell lines used as models for intestinal adhesion

1995

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SummaryAdhesion to HEp-2 cells has been shown to correlate with enteropathogenicity forAeromonasspecies. Such adhesion is thought to reflect the ability of strains to adhere to human intestinal enterocytes, although HEp-2 cells are not of intestinal origin. In this study strains ofAeromonas veroniibiotype sobria isolated from various sources were investigated in parallel assays for their ability to adhere to HEp-2 cells and to an intestinal cell line (Caco-2). Quantitative assays showed identical adhesion values were obtained with both cell lines. Adhesion was best when bacteria were grown at 22 °C compared with 37 °C and 7 °C. Some environmental isolates showed greater adhesion when grown at 7 °C than when grown at 37 °C. Filamentous structures on these strains are also optimally expressed under the above conditions (reported elsewhere). Mechanical shearing or trypsin treatment to remove surface structures from several adhesive strains grown at 22 °C decreased adhesion to cell lines by 50–80% providing further indirect evidence that filamentous adhesins may play a role in cell adhesion for thisAeromonasspecies.

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Section: Discussionmentioning

confidence: 99%

Section: Electron Microscopymentioning

confidence: 99%

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Adhesion ofAeromonassp. to cell lines used as models for intestinal adhesion

1995

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“…Environmental strains of A. veronii biovar sobria are generally more heavily piliated that fecal isolates (23,24). There is some evidence that Bfp promote binding to phagocytic cells, which may represent a selective pressure resulting in a shift in the degree of piliation in vivo (19).…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of Type IV Pili Expressed on Diarrhea-Associated Isolates of Aeromonas species

1999

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Our past work has shown that long, flexible type IV pili (single or in bundles) are the predominant pili expressed on fecal isolates of diarrhea-associated species of Aeromonas (Aeromonas veronii biovar sobria and A. caviae). They represent a family of type IV pili which we have designated Bfp (for bundle-forming pili). Reports from Japan suggest that Bfp are intestinal colonization factors. This study presents compelling evidence to support this conclusion. Aeromonas bacteria and/or Bfp purified from a strain of A. veronii biovar sobria were shown to adhere to epithelial and intestinal cell lines, freshly isolated human enterocytes, and fresh and fixed human and rabbit intestinal tissues, as determined by light and electron microscopy and immunohistochemical detection. Removal of Bfp by mechanical means decreased adhesion to cell lines by up to 80%. Purified Bfp blocked adhesion of the test strain to intestinal cells in a dose-dependent manner. Adhesion was also blocked by the Fab fraction of anti-Bfp immunoglobulin G. Moreover, ultrastructural studies (ruthenium red staining and transmission and scanning electron microscopy) demonstrated for the first time that Aeromonasadhesion to human enterocytes is pilus mediated and suggested that Bfp may also promote colonization by forming bacterium-to-bacterium linkages. Bfp-positive isolates examined for type IV pilus-mediated twitching motility in agar and slide culture assays developed forPseudomonas aeruginosa did not, however, exhibit this function.

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Pili and the interaction of Aeromonas species with humanperipheral blood polymorphonuclear cells

Cited by 2 publications

References 20 publications

Adhesion ofAeromonassp. to cell lines used as models for intestinal adhesion

Adhesion ofAeromonassp. to cell lines used as models for intestinal adhesion

Functional Characterization of Type IV Pili Expressed on Diarrhea-Associated Isolates of Aeromonas species

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