Pili Mediated Agglutination of <i>Serratia marcescens</i> in Human Urine

Amako, Kazunobu; Ohshima, Hisaaki; Yasunaka, Kakuko; Kono, Kenji

doi:10.1111/j.1348-0421.1981.tb00104.x

Cited by 10 publications

(8 citation statements)

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“…has also been reported (2,9). In a previous paper (2) we reported that a strain (US5) of S. marcescens possessing a distinctive type of fimbriae expressing mannose-sensitive (MS) hemagglutination is agglutinated by human urine. This agglutination of bacteria was found to be caused by the interaction of the fimbriae with the mucus present in human urine (6).…”

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confidence: 99%

“…marcescens strain US5 was used as a fimbriated and strain US9 as a nonfimbriated strain. The characteristics of the fimbriae of US5 have been described in a previous paper (2,6,7,10). Strain US9, a clinical isolate, has no fimbrial structure or hemagglutination activity (2).…”

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confidence: 99%

“…The results of this experiment suggest the possibility that MS fimbriae of S. marcescens act as a colonization factor in urinary tract infection. This strain has been reported to interact with urinary mucin and form large aggregates of the bacteria and the mucin in human urine (2,6). The urinary mucin is thought to be derived from the mucous layer of the urinary tract (11,13).…”

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confidence: 99%

“…The characteristics of the fimbriae of US5 have been described in a previous paper (2,6,7,10). Strain US9, a clinical isolate, has no fimbrial structure or hemagglutination activity (2). The pieces of human bladder tissue used in this experiment were taken by a surgeon during an operation for bladder cancer Strain US5 has an MS hemagglutinin and fine flexible fimbriae.…”

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Fimbria‐Mediated Adherence of Serratia marcescens Strain US5 to Human Urinary Bladder Surface

Yamamoto

Ariyoshi

Amako

1985

Microbiology and Immunology

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Gram-negative bacteria isolated from urinary tract infections possess filamentous appendages called fimbriae (3)(4)(5)12). Fimbriae that cause mannose-resistant (MR) hemagglutination are thought to be responsible for the colonization of the bacteria in the urinary tract. Serratia marcescens had once been thought to be a harmless saprophyte. However recent reports implicate this species as the primary pathogen in numerous infections, although the infections are endemic and hospitalaquired or nosocomial (1,8). The presence of fimbriae on Serratia spp. has also been reported (2, 9). In a previous paper (2) we reported that a strain (US5) of S. marcescens possessing a distinctive type of fimbriae expressing mannose-sensitive (MS) hemagglutination is agglutinated by human urine. This agglutination of bacteria was found to be caused by the interaction of the fimbriae with the mucus present in human urine (6). Since the mucus was thought to be produced by the mucus layer of the urinary tract (13), it can be postulated that S. marcescens having this MS type of fimbriae are able to adhere to the human bladder surface and that consequently the fimbriae act as a colonization factor.The adherence of fimbriated bacteria to the urinary tract has been studied with detached uroepithelial cells excreted into urine which was later voided, or with cultured mammalian cells. These in vitro experiments did not give any conclusive evidence, since the surface of the cells used in these experiments was presumed to be different from the surface of the human urinary tract, especially in the presence of the mucus. To obtain more reliable data on the adherence of S. marcescens to the human urinary tract, in this experiment we utilized human urinary bladder tissues which were removed during operations on patients with urinary anomalies or cancer, and the adherence was quantitatively examined with the scanning electron microscope.S. marcescens strain US5 was used as a fimbriated and strain US9 as a nonfimbriated strain. The characteristics of the fimbriae of US5 have been described in a previous paper (2,6,7,10). Strain US9, a clinical isolate, has no fimbrial structure or hemagglutination activity (2). The pieces of human bladder tissue used in this experiment were taken by a surgeon during an operation for bladder cancer 67 7

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Fimbria‐Mediated Adherence of Serratia marcescens Strain US5 to Human Urinary Bladder Surface

Yamamoto

Ariyoshi

Amako

1985

Microbiology and Immunology

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“…Serratia marcescens is an opportunistic organism that may cause nosocomial urinary and respiratory tract infections (2,7). The fimbriae of this species have been reported to be a colonization factor in such urinary tract infections (39).…”

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A cryptic fimbrial gene in Serratia marcescens

et al. 1989

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The gene coding for the mannose-sensitive hemagglutinating fimbriae in Serratia marcescens US5 was cloned into Escherichia coli K4 with a cosmid vector system. One of the transformants, US5-1, expressed two morphologically distinct fimbriae, one that was 5-nm wide and one that was 3-nm wide. The latter fimbria was morphologically and serologically indistinguishable from that of strain US5. Genetic analysis of transformant US5-1 showed that the gene responsible for the 5-nm-wide fimbriae was located more than 10 kilobases away from the gene responsible for the 3-nm-wide fimbriae. The molecular sizes of the subunits of these two fimbriae, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, were 19 kilodaltons for the 3-nm-wide fimbriae and 20 kilodaltons for the 5-nm-wide fimbriae. Serologically, the 5-nm-wide fimbriae did not cross-react with monoclonal or polyclonal antibodies raised against the mannose-sensitive hemagglutinating fimbriae of strain US5. Strain EL101, which expressed only the 5-nm-wide fimbriae, did not agglutinate chicken or human erythrocytes. These experimental results suggest that the gene for the 5-nm-wide fimbriae is cryptic in strain US5 and is expressed in E. coli K4 only after it is moved by transformation.

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Increased renal scarring by bacteria with mannose-sensitive pili

et al. 1990

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Renal scars are thought to be the end stage of chronic pyelonephritis and one of the most important causes of renal insufficiency and renal hypertension. The role of bacterial pili was examined in scar formation after an infection of newly constructed bacterial strains using the recombinant DNA technique, which possessed either mannose resistant (MR) or mannose sensitive (MS) pili of Serratia marcescens. Strains that differed in only a single virulence factor, namely, MR or MS pili, were used in a rat model of chronic pyelonephritis. In this model, MS-piliated bacteria stimulated renal scarring more severely than non-piliated or MR-piliated bacteria.

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Pili Mediated Agglutination of Serratia marcescens in Human Urine

Cited by 10 publications

References 14 publications

Fimbria‐Mediated Adherence of Serratia marcescens Strain US5 to Human Urinary Bladder Surface

Fimbria‐Mediated Adherence of Serratia marcescens Strain US5 to Human Urinary Bladder Surface

A cryptic fimbrial gene in Serratia marcescens

Increased renal scarring by bacteria with mannose-sensitive pili

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