Pilose antler extract restores type I and III collagen to accelerate wound healing

Li, Lishuang; Ma, Yuman; He, Gaiying; Ma, Shugen; Wang, Yi; Sun, Yanan

doi:10.1016/j.biopha.2023.114510

Cited by 23 publications

(6 citation statements)

References 30 publications

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“…However, the decrease in the rate of type V collagen synthesis to one order of magnitude more than those of types I and III collagen may indicate its ability to reinforce the structural composition of these fibrillar collagen, a process that is apparently independent of the coating composition. These findings are in line with studies revealing the interplay of profibrotic conditions and fibronectin on collagen fibrillogenesis [38,64]. In murine mice, the role of type V collagen was speculated for the first time in vivo and discussed in terms of the key regulator factor for collagen fibrillogenesis [2].…”

Section: Modulator Of Fibrillar Collagen Synthesissupporting

confidence: 83%

Vitamin C Regulates the Profibrotic Activity of Fibroblasts in In Vitro Replica Settings of Myocardial Infarction

Zheng

Nilcham

et al. 2023

IJMS

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Extracellular collagen remodeling is one of the central mechanisms responsible for the structural and compositional coherence of myocardium in patients undergoing myocardial infarction (MI). Activated primary cardiac fibroblasts following myocardial infarction are extensively investigated to establish anti-fibrotic therapies to improve left ventricular remodeling. To systematically assess vitamin C functions as a potential modulator involved in collagen fibrillogenesis in an in vitro model mimicking heart tissue healing after MI. Mouse primary cardiac fibroblasts were isolated from wild-type C57BL/6 mice and cultured under normal and profibrotic (hypoxic + transforming growth factor beta 1) conditions on freshly prepared coatings mimicking extracellular matrix (ECM) remodeling during healing after an MI. At 10 μg/mL, vitamin C reprogramed the respiratory mitochondrial metabolism, which is effectively associated with a more increased accumulation of intracellular reactive oxygen species (iROS) than the number of those generated by mitochondrial reactive oxygen species (mROS). The mRNA/protein expression of subtypes I, III collagen, and fibroblasts differentiations markers were upregulated over time, particularly in the presence of vitamin C. The collagen substrate potentiated the modulator role of vitamin C in reinforcing the structure of types I and III collagen synthesis by reducing collagen V expression in a timely manner, which is important in the initiation of fibrillogenesis. Altogether, our study evidenced the synergistic function of vitamin C at an optimum dose on maintaining the equilibrium functionality of radical scavenger and gene transcription, which are important in the initial phases after healing after an MI, while modulating the synthesis of de novo collagen fibrils, which is important in the final stage of tissue healing.

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Section: Modulator Of Fibrillar Collagen Synthesissupporting

confidence: 83%

Vitamin C Regulates the Profibrotic Activity of Fibroblasts in In Vitro Replica Settings of Myocardial Infarction

Zheng

Nilcham

et al. 2023

IJMS

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“…By further analysis of the nutritional composition of the antlers, we found that supplementation with methionine did significantly increase the crude protein and collagen content of plum deer antlers, while significantly decreasing the calcium content of the antlers. As the most abundant active ingredients in deer antler, antler protein and antler polypeptide have various medical effects in clinical practice, including improving immune function and antioxidant levels in animals [ 32 , 33 ], resisting acetaminophen-induced nephrotoxicity [ 34 ], improving neurological damage and cognitive dysfunction [ 35 ], repairing vascular and epithelial tissue damage and accelerating wound healing [ 36 ]. Calcium deposition as a landmark process of antler aging and the calcium and collagen content of antler, showed a significant linear relationship with the freshness of the antler, and the higher the degree of ossification the corresponding decrease in medicinal value [ 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Methionine Supplementation Affects Fecal Bacterial Community and Production Performance in Sika Deer (Cervus nippon)

Yan

Zhu

Guo

et al. 2023

Animals

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Amino acid balance is central to improving the efficiency of feed protein utilization and for reducing environmental pollution caused by intensive farming. In previous studies, supplementation with limiting amino acids has been shown to be an effective means of improving animal nutrient utilization and performance. In this experiment, the effects of methionine on the apparent digestibility of nutrients, antler nutrient composition, rumen fluid amino acid composition, fecal volatile fatty acids and intestinal bacteria in antler-growing sika deer were investigated by randomly adding different levels of methionine to the diets of three groups of four deer at 0 g/day (CON), 4 g/day (LMet) and 6 g/day (HMet). Methionine supplementation significantly increased the apparent digestibility of organic matter, neutral detergent fiber (NDF) and acid detergent fiber (ADF) in the LMet group (p < 0.05). The crude protein and collagen protein of antlers were significantly higher in the LMet and HMet groups compared to the CON group and also significantly higher in the HMet group compared to the LMet group, while the calcium content of antlers was significantly lower in the HMet group (p < 0.05). Ruminal fluid free amino acid composition was altered in the three groups of sika deer, with significant changes in aspartic acid, citrulline, valine, cysteine, methionine, histidine and proline. At the phylum level, Firmicutes and Bacteroidetes were highest in the rectal microflora. Unidentified bacterial abundance was significantly decreased in the HMet group compared to the CON group. Based on the results of principal coordinate analysis (PCoA) and Adonis analysis, there was a significant difference in the composition of the intestinal flora between the CON and HMet groups (p < 0.05). At the genus level, compared with the CON group, the abundance of Rikenellaceae_RC9_gut_group and Lachnospiraceae_UCG-010 in the LMet group increased significantly (p < 0.05), the abundance of dgA-11_gut_group in the HMet group decreased significantly (p < 0.05) and the abundance of Lachnospiraceae_UCG-010, Saccharofermentans and Lachnospiraceae_NK3A20_group increased significantly. Taken together, the results showed that methionine supplementation was beneficial in increasing the feed utilization efficiency and improving antler quality in sika deer, while affecting the composition of fecal bacteria.

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“…Lignin powder (1.2–1.4 g) was mixed in 2 mL deionized (DI) water +2 mL KOH 2 M (ratio 1:1 v/v) by stirring until the mixture was completely dissolved into a solution. The amount of PEG.DGE added to lignin (with increasing lignin/PEG.DGE mass ratios, specifically from 12:9 to 14:9 (w/w) for the lignin-low, lignin-medium, and lignin-high hydrogels) was incorporated into the vial. − After stirring the mixture at 600 rpm/min for 1 h, the reaction mixture was transferred into a 1 mm-thick glass mold. The condensation polymers of the lignin gel were monitored for 12 h, after which the lignin gel was removed from the mold.…”

Section: Methodsmentioning

confidence: 99%

Lignin-Based Antioxidant Hydrogel Patch for the Management of Atopic Dermatitis by Mitigating Oxidative Stress in the Skin

Trinh,

Nguyen,

Kim

2024

ACS Appl. Mater. Interfaces

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Atopic dermatitis (AD), a chronic skin condition characterized by itching, redness, and inflammation, is closely associated with heightened levels of endogenous reactive oxygen species (ROS) in the skin. ROS can contribute to the onset and progression of AD through oxidative stress, which leads to the release of proinflammatory cytokines, T-cell differentiation, and the exacerbation of skin symptoms. In this study, we aim to develop a therapeutic antioxidant hydrogel patch for the potential treatment of AD using lignin, a biomass waste material. Lignin contains polyphenol groups that enable it to scavenge ROS and exhibit antioxidant properties. The lignin hydrogel patches, possessing optimized mechanical properties through the control of the lignin and cross-linker ratio, demonstrated high ROS-scavenging capabilities. Furthermore, the lignin hydrogel demonstrated excellent biocompatibility with the skin, exhibiting beneficial properties in protecting human keratinocytes under high oxidative conditions. When applied to an AD mouse model, the hydrogel patch effectively reduced epidermal thickness in inflamed regions, decreased mast cell infiltration, and regulated inflammatory cytokine levels. These findings collectively suggest that lignin serves as a therapeutic hydrogel patch for managing AD by modulating oxidative stress through its ROS-scavenging ability.

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Pilose antler extract restores type I and III collagen to accelerate wound healing

Cited by 23 publications

References 30 publications

Vitamin C Regulates the Profibrotic Activity of Fibroblasts in In Vitro Replica Settings of Myocardial Infarction

Vitamin C Regulates the Profibrotic Activity of Fibroblasts in In Vitro Replica Settings of Myocardial Infarction

Methionine Supplementation Affects Fecal Bacterial Community and Production Performance in Sika Deer (Cervus nippon)

Lignin-Based Antioxidant Hydrogel Patch for the Management of Atopic Dermatitis by Mitigating Oxidative Stress in the Skin

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