A new Nocardiopsis species that degrades polylactic acid (PLA) was isolated from pig dung-based compost from a municipal composting facility in Japan. To obtain strains capable of e cient PLA degradation, we minimized the effect of non-enzymatic degradation of PLA by maintaining the temperature at 37°C or below. After screening a total of 15 animal waste-based compost samples, consisting of pig dung, cow dung, horse dung, or chicken droppings, we found that compost derived from pig dung was most e cient for degradation of PLA lm, and used it for isolation of PLA-degrading microorganisms. Screening for PLA-degrading microorganisms in compost was performed using an agar plate-based method; an emulsi er was omitted to avoid selection of strains that assimilated the emulsi er instead of PLA in the medium. After repeated enrichment, six strains were obtained. One strain that exhibited stable PLA degradation on agar plates was subjected to genomic analysis and identi ed as Nocardiopsis chromatogenes, an actinomycete.