Pilot-scale production of antibacterial substances by the marine diatom Phaeodactylum tricornutum Bohlin

Wang, Song; Said, Inamullah Hakeem; Thorstenson, Candice; Thomsen, Claudia; Ullrich, Matthias S.; Kuhnert, Nikolai; Thomsen, Laurenz

doi:10.1016/j.algal.2018.03.014

Cited by 21 publications

(10 citation statements)

References 41 publications

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“…Furthermore, diatoms are abundant in bioactive metabolites such as antibacterial polyunsaturated fatty acids, i.e. eicosapentaenoic acid [ 5 ], and photosynthetic accessory pigments, such as fucoxanthin, both of which have been heavily investigated over the last several decades [ 6 ]. Previously, we identified and purified the benthic diatom, Cylindrotheca closterium , which showed outstanding characteristics in growth and lipid content [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

et al. 2018

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BackgroundMarine diatoms have a higher fucoxanthin content in comparison to macroalgae. Fucoxanthin features many potent bioactive properties, particularly anti-obesity properties. Despite the great potential for harvesting larger amounts of fucoxanthin, the impacts of light quality (light source, intensity, and photoperiod) on fucoxanthin production and the essential proteins involved in fucoxanthin biosynthesis in marine diatoms remain unclear.ResultsIn the present study, Cylindrotheca closterium was selected from four different species of diatoms based on its high fucoxanthin content and productivity. Optimal light conditions (light source, intensity, and regime) were determined by a “Design of Experiment” approach (software MODDE Pro 11 was used). The model indicated that an 18/6 light/darkness regime increased fucoxanthin productivity remarkably as opposed to a 12/12 or 24/0 regime. Eventually, blue light-emitting diode light, as an alternative to fluorescent light, at 100 μmol/m2/s and 18/6 light/darkness regime yielded maximum fucoxanthin productivity and minimal energy consumption. The fucoxanthin production of C. closterium under the predicted optimal light conditions was assessed both in bottle and bag photobioreactors (PBRs). The high fucoxanthin content (25.5 mg/g) obtained from bag PBRs demonstrated the feasibility of large-scale production. The proteomes of C. closterium under the most favorable and unfavorable fucoxanthin biosynthesis light/darkness regimes (18/6 and 24/0, respectively) were compared to identify the essential proteins associated with fucoxanthin accumulation by matrix-assisted laser desorption/ionization-time of flight–mass spectrometry. Six proteins that were up-regulated in the 18/6 regime but down-regulated in the 24/0 were identified as important chloroplastic proteins involved in photosynthesis, energy metabolism, and cellular processes.ConclusionsBlue light-emitting diode light at 100 μmol/m2/s and 18/6 light/darkness regime induced maximum fucoxanthin productivity in C. closterium and minimized energy consumption. The high fucoxanthin production in the bag photobioreactor under optimal light conditions demonstrated the possibility of commercialization. Proteomics suggests that fucoxanthin biosynthesis is intimately associated with the photosynthetic efficiency of the diatom, providing another technical and bioengineering outlook on fucoxanthin enhancement.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-0957-0) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

et al. 2018

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“…Moreover, using diatom species in the fermentation approaches for the pilot-scale production seems to be promoting in many industrial fields such as antimicrobial production [133]. Interestingly, these species modified by genetic engineering methods can grow in photoautotrophic conditions using suitable photobioreactors [38]. However, the production cost of the autotrophic diatoms is still rather high.…”

Section: Issues and Challengesmentioning

confidence: 99%

“…When we focus on the increase of the diatoms high-value products generated by the time thanks to the genetic transformation, we must confirm that this tool could revolutionize the blue biotechnology. Nevertheless, the low metabolic rate efficiency of these diatom species limits their applications in biotechnology [38] [193] [194]. Besides, transgenic microorganisms are supposed to be monitored tightly for the safety of the ecosystem.…”

Section: Issues and Challengesmentioning

confidence: 99%

“…These antimicrobial products are needed as an alternative to chemical and not efficient current products for overcoming different microbial diseases. Nowadays, the pharmaceutical industry is looking for new compounds to be produced on a large scale as a substitute for traditional antibiotics that became worthless with antibiotic-resistant traits of microbes [38]. Recent studies have confirmed that diatoms such as P. tricornutum seem to be of great potential to produce antimicrobial compounds such as eicosapentaenoic acid (EPA), hexadecenoic acid and, hexadecatrienoic acid.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have confirmed that diatoms such as P. tricornutum seem to be of great potential to produce antimicrobial compounds such as eicosapentaenoic acid (EPA), hexadecenoic acid and, hexadecatrienoic acid. These unsaturated fatty acids were identified using analytical techniques such as mass spectrometry and nuclear magnetic resonance spectroscopy [38] [39] [40] [41].…”

Section: Introductionmentioning

confidence: 99%

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Diatoms’ Breakthroughs in Biotechnology: <i>Phaeodactylum tricornutum</i> as a Model for Producing High-Added Value Molecules

Dhaouadi¹,

Awwad²,

Diamond³

et al. 2020

AJPS

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With a world growing in population and nutritional needs, diatoms are considered nowadays as microalgae of a very important potential, thus they are exploited in several fields such as ecology, aquaculture, molecular farming, and pharma nutraceuticals. These coveted microalgae are characterized by their diversity, their high division rates, their complex life cycle, likewise their silicified cell walls named frustules. Thus, diatoms have been used for over a century after proving an efficient production of several molecules including Triacylglycerols (TAGs), H 2 , free fatty acids, vitamins, nutraceuticals, amino acids, proteins, terpenoids, alcohols and carbohydrates like starch, glycogen, and sucrose. Phaeodactylum tricornutum is the most promising diatom exploited to date, especially as a platform of pharmaceutical production. Herein, we expose diatoms' main features that allowed using them for molecular farming. This review exposes likewise, the metabolism and the post-translational modifications (PTMs) of diatoms as well as current tools and challenges for their molecular and metabolic engineering for a more efficient production of valuable molecules. The knowledge on the biology of the diatoms, the molecular tools, and the various transformation methods available demonstrate the potential in biotechnology of these photosynthetic microorganisms. The widely studied P. tricornutum, as a model organism, is a promising diatom for production of valuable metabolites, despite the challenges and issues related to cultivation.

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Bioactive Compounds from Microalgae and Their Potential Applications as Pharmaceuticals and Nutraceuticals

Chu

Phang

2019

Grand Challenges in Algae Biotechnology

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Pilot-scale production of antibacterial substances by the marine diatom Phaeodactylum tricornutum Bohlin

Cited by 21 publications

References 41 publications

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

Diatoms’ Breakthroughs in Biotechnology: <i>Phaeodactylum tricornutum</i> as a Model for Producing High-Added Value Molecules

Bioactive Compounds from Microalgae and Their Potential Applications as Pharmaceuticals and Nutraceuticals

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Pilot-scale production of antibacterial substances by the marine diatom Phaeodactylum tricornutum Bohlin

Cited by 21 publications

References 41 publications

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light

Diatoms’ Breakthroughs in Biotechnology: &lt;i&gt;Phaeodactylum tricornutum&lt;/i&gt; as a Model for Producing High-Added Value Molecules

Bioactive Compounds from Microalgae and Their Potential Applications as Pharmaceuticals and Nutraceuticals

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Diatoms’ Breakthroughs in Biotechnology: <i>Phaeodactylum tricornutum</i> as a Model for Producing High-Added Value Molecules