Pilot-scale production of lutein using Chlorella vulgaris

McClure, Dale D.; Nightingale, Jonathan K.; Luiz, Audrey; Black, Sachin; Zhu, Jingyuan; Kavanagh, John M.

doi:10.1016/j.algal.2019.101707

Cited by 33 publications

(12 citation statements)

References 35 publications

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“…The biomass productivity was 0.095±0.014 g/L/day, and the specific growth was 0.5 day -1 . This result correlates with findings from studies of the effect of light intensity on the growth of C. vulgaris biomass; 0.36 day -1 (Nguyen et al, 2019), 0.65 day -1 (Araya et al, 2014), 1.0-1.24 day -1 (McClure et al, 2019). Those studies showed a specific increase in the growth rate with respect to light intensity (Araya et al, 2014;Nguyen et al, 2019;McClure et al, 2019).…”

Section: Calculation: Specific Growth Rate Biomass Productivity Andsupporting

confidence: 90%

The Effects of Solvents and Solid-to-Solvent Ratios on Ultrasound-Assisted Extraction of Carotenoids from Chlorella vulgaris

Dianursanti

Siregar

Maeda

et al. 2020

IJTech

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Research around using algae as a natural source of carotenoids has been intense in the 21st century, given the wide applications of carotenoids in the pharmaceutical, health, and food industries. This study aimed to get the highest yield of carotenoids from Chlorella vulgaris by ultrasound extraction. This study evaluated two parameters: the extraction solvent (ethanol, acetone, and diethyl ether were tested) and the solid-to-solvent ratio (1:30, 1:50, and 1:100 were tested). The carotenoid extracted from C. vulgaris was lutein, and its compounds were identified by UV-Vis spectroscopy. The highest carotenoid yield was achieved using ethanol at 1.146±0.082 mg/g and a solid-to-solvent ratio of 1:100 (g/mL). This research shows the use of a specific extraction solvent along with a solid-to-solvent ratio is significant to determine carotenoids yield desired. Further study of other parameters (e.g., temperature and ultrasound intensity) is necessary for the optimum extraction condition.

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Section: Calculation: Specific Growth Rate Biomass Productivity Andsupporting

confidence: 90%

The Effects of Solvents and Solid-to-Solvent Ratios on Ultrasound-Assisted Extraction of Carotenoids from Chlorella vulgaris

Dianursanti

Siregar

Maeda

et al. 2020

IJTech

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“…Lutein sales amount to 150 million dollars in the USA only. Currently, lutein is obtained from marigold petals, with a low lutein content of 0.03% (w/w) [10,11]. Actually, the only possible other sources with sufficient content to be considered for lutein production are certain strains of microalgae, several of them considered as potential lutein sources based on their lutein content which ranges from 0.5% to 1.2% dry weight [10].…”

Section: Introductionmentioning

confidence: 99%

Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production

et al. 2020

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The large-scale biomass production is an essential step in the biotechnological applications of microalgae. Coccomyxa onubensis is an acidophilic microalga isolated from the highly acidic waters of Río Tinto (province of Huelva, Spain) and has been shown to accumulate a high concentration of lutein (9.7 mg g−1dw), a valuable antioxidant, when grown at laboratory-scale. A productivity of 0.14 g L−1 d−1 was obtained by growing the microalga under outdoor conditions in an 800 L tubular photobioreactor. The results show a stable biomass production for at least one month and with a lutein content of 10 mg g−1dw, at pH values in the range 2.5–3.0 and temperature in the range 10–25 °C. Culture density, temperature, and CO2 availability in highly acidic medium are rate-limiting conditions for the microalgal growth. These aspects are discussed in this paper in order to improve the outdoor culture conditions for competitive applications of C. onubensis.

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“…Wei achieved 1.98 mg/g DW lutein content in C. protothecoides [39], but Chen reached 5.88 mg/g DW lutein with two-stage heterotrophic culture of C. sorokiniana [38]. Lutein content in C. minutissima reached 8.24 mg/g DW in Dineshkumar's study [40], but the highest extraction reported was 10.4 ± 5.5 mg/g DW in C. vulgaris in McClure's study using photoautotrophic cultivation mode [41]. In contrast, very low lutein was reported in Othman's study.…”

Section: High-value Productsmentioning

confidence: 80%

“…On the other hand, a thick cell wall makes Chlorella shear resistant and is an advantage for cultivation in bioreactors. Numerous studies show that Chlorella species hold great potential for a large-scale biomass production, however optimization of cultivation conditions is of primary importance to achieve high biomass yield and increase the content of high value compounds ANNEX [14], [15], [47], [48], [56], [68], [1], [17], [41], [55], [57], [61] Chlorophyll Lutein [31], [60], [74], [76], [78]- [80], [100], [101], [77], [ [64], [65], [95], [97] NA -data not available.…”

Section: Discussionmentioning

confidence: 99%

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Potential of Chlorella Species as Feedstock for Bioenergy Production: A Review

Ieviņa

Romagnoli

2020

Environmental and Climate Technologies

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Selection of appropriate microalgae strain for cultivation is essential for overall success of large-scale biomass production under particular environmental and climate conditions. In addition to fast growth rate and biomass productivity, the species ability to grow in wastewater must also be considered to increase the economic feasibility of microalgae for bioenergy purposes. Furthermore, the content of bioactive compounds in a strain must be taken into account to further increase the viability by integration of biorefinery concept. Chlorella spp. are among the most studied microalgal species. The present review attempts to unfold the potential of species of the genus Chlorella for bioenergy production integrating applicability for wastewater treatment and production of high added-value compounds. Several key features potentially make Chlorella spp. highly beneficial for bioenergy production. Fast growth rate, low nutritional requirements, low sensitivity to contamination, adaptation to fluctuating environments, ability to grow in photoautotrophic, heterotrophic and mixotrophic conditions make Chlorella spp. highly useful for outdoor cultivation coupled with wastewater treatment. Chlorella is a source of multiple bioactive compounds. Most promising high-value products are chlorophylls, lutein, β-carotene and lipids. Here we demonstrate that although many Chlorella spp. show similar characteristics, some substantial differences in growth and response to environmental factors exist.

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Pilot-scale production of lutein using Chlorella vulgaris

Cited by 33 publications

References 35 publications

The Effects of Solvents and Solid-to-Solvent Ratios on Ultrasound-Assisted Extraction of Carotenoids from Chlorella vulgaris

The Effects of Solvents and Solid-to-Solvent Ratios on Ultrasound-Assisted Extraction of Carotenoids from Chlorella vulgaris

Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production

Potential of Chlorella Species as Feedstock for Bioenergy Production: A Review

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