Pilot-Scale Protease Production by <i>Bacillus</i> sp. C4 for Silk Degumming Processes

Prathumpai, Wai; Promboon, Amornrat; Werapan, Borworn; Nutaratat, Pumin; Chim-Anek, Patoomporn; Ninpetch, Uraiwan

doi:10.1080/15440478.2020.1788482

Cited by 10 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Even so, the degumming effectiveness of the protease was moderately underneath than the Na 2 CO 3 . But the SEM image ensured that the degummed thread was an excellent result than Na 2 CO 3 (0.05%) degummed by using protease as a degumming factor [66]. The degumming efficacy of the Bacillus sp.…”

Section: Various Micro-organism Speciesmentioning

confidence: 97%

Extraction of Silk Fibroin with Several Sericin Removal Processes and its Importance in Tissue Engineering: A Review

et al. 2022

View full text Add to dashboard Cite

Sericin is a sticky protein substance, which is generated by the silk-worm for holding the filaments of silk tightly. The main constituent of natural crude silk is made up of sericin and fibroin. Fibroin and sericin play a vital role in increasing the strength, stiffness and on maintaining the constructional integrality of the cocoon. This review reports the role of several types of degumming agents for the removal of sericin from silk surfaces and an overview of silk fibroin approaches in the tissue engineering field. The prime factors responsible for the removal of sericin (degumming) from silk were systematically analyzed and discussed. The key factors affects on modification of the silk surface are includes surfactant, degumming time, temperature, advanced techniques such as ultra-sonication, microwave radiation, infrared radiation, low-temperature oxygen plasma radiation, and PH. These factors influence either individually or cumulatively at the degumming process. Tissue engineering is an emanate and most optimistic approach to restore tissue malfunction by adopting the concept of the technology being practiced using numerous biomaterials, cells, and the nutrient-specific growth factors. Different types of materials include various bio-material and mainly of silk fibroin constituent of it is conceding most optimistic biomaterials in tissue engineering application. Silk fibroin is a naturalistic protein-based polymer with an eminent physiochemical characteristic, are magnificent biocompatibility, amenable biodegradability, proper oxygen, and water-vapor permeability, and least inflammatory reaction. The critical studies accomplishing on the role of Silk fibroin and various approaches adopted in the degumming process is extensively reviewed and reported.

show abstract

Section: Various Micro-organism Speciesmentioning

confidence: 97%

Extraction of Silk Fibroin with Several Sericin Removal Processes and its Importance in Tissue Engineering: A Review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…LA-05 [33], an enzyme from natural sources such as papaya skin, pineapple skin and guava leaf [34], protease from Bacillus sp. C4 [35], hydrated lime water [36], and alkyl polyglycoside [37] have been explored for treatment. Microwave-assisted silk degumming was carried out by Haggag et al [38] and Mahmoodi et al [39].…”

Section: Degumming Of Raw Silkmentioning

confidence: 99%

Review on Fabrication and Application of Regenerated Bombyx mori Silk Fibroin Materials

Wang

Zhou

et al. 2023

AUTEX Research Journal

View full text Add to dashboard Cite

Natural silk fiber derived from the Bombyx mori (B. mori) silkworm has long been used as a luxury raw material in textile industry because of its shimmering appearance and durability, and as surgical suture for its high strength and flexibility. Regenerated silk fibroin, as the main protein extracted from the cocoons of the B. mori silkworm, recently has gained considerable attention due to its outstanding properties, including facile processability, superior biocompatibility, controllable biodegradation, and versatile functionalization. Tremendous effort has been made to fabricate silk fibroin into various promising materials with controlled structural and functional characteristics for advanced utilities in a multitude of biomedical applications, flexible optics, electronics devices, and filtration systems. Herein, reverse engineered silk fibroin extraction methods are reviewed, recent advances in extraction techniques are discussed. Fabrication methods of silk fibroin materials in various formats are also addressed in detail; in particular, progress in new fabrication technologies is presented. Attractive applications of silk fibroin-based materials are then summarized and highlighted. The challenges faced by current approaches in production of silk fibroin-based materials and future directions acquired for pushing these favorable materials further toward above mentioned applications are further elaborated.

show abstract

“…Prathumpai et al [ 84 ] developed a pilot-scale production of serine protease from Bacillus sp. C-4, which has been reported as a candidate protease in the degumming process of the silk industry.…”

Section: Introductionmentioning

confidence: 99%

“…The efficiency of the degumming process was approximately 81% when considering the alkaline degumming process. The protease can be conveniently preserved at room temperature for up to one month and remains stable when refrigerated for over two months without the need for chemical additives or cryoprotectants [ 84 ].…”

Section: Introductionmentioning

confidence: 99%

Sustainable Silk-Based Particulate Systems for the Controlled Release of Pharmaceuticals and Bioactive Agents in Wound Healing and Skin Regeneration

Bernardes,

Veiga,

Barros

et al. 2024

IJMS

View full text Add to dashboard Cite

The increasing demand for innovative approaches in wound healing and skin regeneration has prompted extensive research into advanced biomaterials. This review focuses on showcasing the unique properties of sustainable silk-based particulate systems in promoting the controlled release of pharmaceuticals and bioactive agents in the context of wound healing and skin regeneration. Silk fibroin and sericin are derived from well-established silkworm production and constitute a unique biocompatible and biodegradable protein platform for the development of drug delivery systems. The controlled release of therapeutic compounds from silk-based particulate systems not only ensures optimal bioavailability but also addresses the challenges associated with conventional delivery methods. The multifaceted benefits of silk proteins, including their inherent biocompatibility, versatility, and sustainability, are explored in this review. Furthermore, the intricate mechanisms by which controlled drug release takes place from silk-based carriers are discussed.

show abstract

Pilot-Scale Protease Production by Bacillus sp. C4 for Silk Degumming Processes

Cited by 10 publications

References 24 publications

Extraction of Silk Fibroin with Several Sericin Removal Processes and its Importance in Tissue Engineering: A Review

Extraction of Silk Fibroin with Several Sericin Removal Processes and its Importance in Tissue Engineering: A Review

Review on Fabrication and Application of Regenerated Bombyx mori Silk Fibroin Materials

Sustainable Silk-Based Particulate Systems for the Controlled Release of Pharmaceuticals and Bioactive Agents in Wound Healing and Skin Regeneration

Contact Info

Product

Resources

About