Pilot-Scale Recovery of Phycoerythrin fromPorphyridium cruentumusing Expanded Bed Adsorption Chromatography

Bermejo, Ruperto; Ruiz, Encarnación; Ramos, Amparo; Acién, F.G.

doi:10.1080/01496395.2013.791319

Cited by 18 publications

(3 citation statements)

References 28 publications

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“…has been reported [6][7][8][9][10]. Various chromatography methods, such as ion chromatography (IC) and gel permeation chromatography (GPC), are used for the separation of PB [10,[13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Prediction of Concentration and Productivity of Phycobiliprotein from Nostoc commune by UF Membrane Modules

et al. 2023

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Phycobiliprotein (PB) produced by cyanobacteria exhibits an anti-inflammatory effect and anticancer effect, and is expected to be applied as pharmaceuticals. In this study, a PB-containing solution from Nostoc commune was concentrated and separated by an ultrafiltration (UF) membrane module (nominal molecular weight limit (NMWL) 150 kDa, 30 kDa and 10 kDa). When the PB-containing solution was permeated through an UF membrane module of NMWL 10 kDa at 0.07 MPa, the total protein content was concentrated up to 10 times in 30 minutes. To simulate the change in the concentration for the concentrate in the scaled-up treatment, the parameters of the formed cake layer were determined by a mathematical model. The concentration performance of the UF membrane modules was evaluated using the obtained parameters, indicating that the 150 kDa NMWL UF membrane modules were able to concentrate 100 liters of PB-containing solution by 10 times in 15 hours. The 30 kDa and 10 kDa NMWL UF membrane modules showed the possibility of PB separation, as well as protein concentration. The productivity of PB calculated by flux and selectivity was highest for the 30 kDa NMWL UF membrane module.

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Section: Introductionmentioning

confidence: 99%

Prediction of Concentration and Productivity of Phycobiliprotein from Nostoc commune by UF Membrane Modules

et al. 2023

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“…Therefore, the process of PE puri cation should be tailored to its expected applications. Different non-chromatographic and chromatographic methods have been developed to purify PE from both microalgae and macroalgae (Bermejo et al 2013). However, simplicity, yield, cost, scale-up issues, selectivity, molecular structure of PE, and complicated process create differences between these puri cation methods.…”

Section: Introductionmentioning

confidence: 99%

Efficient Purification and Characterization of Phycoerythrin from Caspian Sea Red Macroalgae (Osmundea caspica)

Eshaghzadeh

Shahbazi

Akhavan

et al. 2022

Preprint

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Phycoerythrin (red pigment) (PE) is a naturally occurring colorant with excellent uorescent and antioxidant properties that has a wide range of applications. More studies are needed, however, to improve the puri cation yield and chemical properties of PE. This study aimed to compare the use of different puri cation methods, which are activated charcoal (AC), ammonium sulfate precipitation (ASP), or anion-exchange chromatography (IE) alone and a combination of AC, ASP, and IE, for PE extraction from the Caspian Sea red macroalgae (Osmundea caspica). Response surface methodology (RSM) was employed for preliminary puri cation, with the independent variables being activated charcoal content (0.1-1% w/v) and stirring time (2-10 min). With an AC content of 0.4% and a stirring time of 2 min, PE purity and concentration were optimized. The predicted values from the equations agreed well with the experimental values, demonstrating the model's robustness. A three-step increase in the ionic strength of IE was also evaluated at three different treatments (PECE, PECE + AC + IE, and ASP + IE). The highest PE purity and lowest Fluorescence intensity (FI) and Antioxidant activity (AA) were obtained in the fraction of AE-200 (second fraction) with an index of 2.8 and a recovery yield of 48% in ASP + IE, whereas an index purity of 2 and the highest recovery yield of 67% were obtained using PECE + AC + IE.

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“…21-23 It has been reported in many research works about how to efficiently obtain compounds by EBAC. 24-26 EBAC is an efficient technique which integrates extraction and separation in a single step. HSCCC is a favorable method for purification of compounds from crude extract because no solid-supported matrix is used and almost 100% of the sample is recovered.…”

Section: Introductionmentioning

confidence: 99%

Development of a Method to Extract and Purify Target Compounds from Medicinal Plants in a Single Step: Online Hyphenation of Expanded Bed Adsorption Chromatography and Countercurrent Chromatography

Wang

Zhang

et al. 2014

Anal. Chem.

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Pure compounds extracted and purified from natural sources are crucial to lead discovery and drug screening. This study presents a novel two-dimensional hyphenation of expanded bed adsorption chromatography (EBAC) and high-speed countercurrent chromatography (HSCCC) for extraction and purification of target compounds from medicinal plants in a single step. The EBAC and HSCCC were hyphenated via a six-port injection valve as an interface. Fractionation of ingredients of Salvia miltiorrhiza and Rhizoma coptidis was performed on the hyphenated system to verify its efficacy. An amount each of 52.9 mg of salvianolic acid B and 2.1 mg of rosmarinic acid was obtained from Salvia miltiorrhiza by the two-dimensional system in a single step. The purities of the targets were over 96% of salvianolic acid B and 74% of rosmarinic acid. An amount each of 4.6 mg of coptisine and 4.1 mg of berberine was obtained from Rhizoma coptidis each with 98% and 82% purity, respectively. The processing time was nearly 50% that of the multi-step method. These results indicate that the present method is a rapid and green way to harvest targets from medicinal plants in a single step.

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Pilot-Scale Recovery of Phycoerythrin fromPorphyridium cruentumusing Expanded Bed Adsorption Chromatography

Cited by 18 publications

References 28 publications

Prediction of Concentration and Productivity of Phycobiliprotein from <i>Nostoc commune</i> by UF Membrane Modules

Prediction of Concentration and Productivity of Phycobiliprotein from <i>Nostoc commune</i> by UF Membrane Modules

Efficient Purification and Characterization of Phycoerythrin from Caspian Sea Red Macroalgae (Osmundea caspica)

Development of a Method to Extract and Purify Target Compounds from Medicinal Plants in a Single Step: Online Hyphenation of Expanded Bed Adsorption Chromatography and Countercurrent Chromatography

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