Pilot Study for Validation and Differentiation of Alveolar and Esophageal Air

Santos, Paulo Henrique Fernandes dos; Vassilenko, Valentina; Conduto, Carolina; Fernandes, Jorge M. O.; Moura, Pedro Catalão; Bonifácio, Paulo

doi:10.1007/978-3-030-78288-7_32

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…To be more specific, the sum of all the components, also known as the total explained variance, reached levels of 97.9%. If IMS proved to be capable of distinguishing such complex samples almost perfectly, then, its suitability for overall VOCs detection is evident [104].…”

Section: Vocs Identification Through Imsmentioning

confidence: 99%

Ion Mobility Spectrometry Towards Environmental Volatile Organic Compounds Identification and Quantification: a Comparative Overview over Infrared Spectroscopy

Moura

Vassilenko

Ribeiro

2023

Emiss. Control Sci. Technol.

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Volatile organic compounds (VOCs) can be extremely toxic and hazardous to expose humans in both indoor and outdoor environments; thus, their detection, correct identification, and accurate quantification are relevant and demanding tasks that need to be addressed. Fortunately, several known analytical techniques allow the qualitative and quantitative assessment of these compounds. This review paper stresses on two independent spectroscopic techniques, infrared spectroscopy and ion mobility spectrometry, both suitable for the detection of very small concentration levels of VOCs in gaseous samples. Infrared spectroscopy is a well-known technique that has been largely applied per se or combined with additional methodologies, to study VOCs at both high and low concentration levels. On the other hand, ion mobility spectrometry gained relevance in this field, due to its capability to measure trace concentration levels, namely ppbv and even pptv. For this review paper, several scientific papers were analyzed, and the most relevant were addressed throughout the text. The working principles of both techniques are carefully addressed, and updated data is provided for highlighting the advantages and disadvantages of both techniques for the environmental VOCs assessment in air quality control.

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Section: Vocs Identification Through Imsmentioning

confidence: 99%

Ion Mobility Spectrometry Towards Environmental Volatile Organic Compounds Identification and Quantification: a Comparative Overview over Infrared Spectroscopy

Moura

Vassilenko

Ribeiro

2023

Emiss. Control Sci. Technol.

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“…The increasing number of risk factors and activities, like smoking, is leading to an equal increase in oesophageal cancer incidence. The identification of biomarkers in the exhaled breath of patients has been deeply studied regarding its potential for a rapid, non-invasive, and accurate diagnosis [148,154,155].…”

Section: Oesophageal Cancermentioning

confidence: 99%

Diagnosis of Carcinogenic Pathologies through Breath Biomarkers: Present and Future Trends

Vassilenko,

Moura,

Raposo

2023

Biomedicines

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The assessment of volatile breath biomarkers has been targeted with a lot of interest by the scientific and medical communities during the past decades due to their suitability for an accurate, painless, non-invasive, and rapid diagnosis of health states and pathological conditions. This paper reviews the most relevant bibliographic sources aiming to gather the most pertinent volatile organic compounds (VOCs) already identified as putative cancer biomarkers. Here, a total of 265 VOCs and the respective bibliographic sources are addressed regarding their scientifically proven suitability to diagnose a total of six carcinogenic diseases, namely lung, breast, gastric, colorectal, prostate, and squamous cell (oesophageal and laryngeal) cancers. In addition, future trends in the identification of five other forms of cancer, such as bladder, liver, ovarian, pancreatic, and thyroid cancer, through perspective volatile breath biomarkers are equally presented and discussed. All the results already achieved in the detection, identification, and quantification of endogenous metabolites produced by all kinds of normal and abnormal processes in the human body denote a promising and auspicious future for this alternative diagnostic tool, whose future passes by the development and employment of newer and more accurate collection and analysis techniques, and the certification for utilisation in real clinical scenarios.

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“…The remaining part of breath used for analysis is composed of end-tidal or alveolar air. This portion of exhaled air contains a higher concentration of endogenous VOCs that originate from blood-gas exchange in the alveoli and thus more closely reflect metabolic conditions (Jia et al 2019, Santos et al 2021. When polymer bags are used, a mixed expiratory breath is usually collected.…”

Section: Introductionmentioning

confidence: 99%

Impact of breath sample collection method and length of storage of breath samples in Tedlar bags on the level of selected volatiles assessed using gas chromatography-ion mobility spectrometry (GC-IMS)

Czippelová,

Nováková,

Šarlinová

et al. 2024

J. Breath Res.

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The analysis of volatile organic compounds (VOCs) in exhaled air has attracted the interest of the scientific community because it provides the possibility of monitoring physiological and metabolic processes and non-invasive diagnostics of various diseases. However, this method remains underused in clinical practice as well as in research because of the lack of standardized procedures for the collection, storage and transport of breath samples, which would guarantee good reproducibility and comparability of results. The method of sampling, as well as the storage time of the breath samples in the polymer bags used for sample storage and transport, affect the composition and concentration of VOCs present in the breath samples. The aim of our study was to compare breath samples obtained using two methods with fully disposable equipment: Haldane sampling tube intended for direct breath collection and breath samples exhaled into a transparent Tedlar bag. The second task was to monitor the stability of selected compounds of real breath samples stored in a Tedlar bag for 6 hours. Gas chromatography - ion mobility spectrometry (GC-IMS) implemented in the BreathSpec® device was used to analyse exhaled breath. Our results showed a significant difference in the signal intensity of some volatiles when taking a breath sample with a Haldane tube and a Tedlar bag. Due to its endogenous origin, acetone levels were significantly higher when the Haldane tube sampler was used while elevated levels of 2-propanol and unidentified VOC in the Tedlar bag samples likely originated from contamination of the Tedlar bags. The VOC stability study revealed compound-specific signal intensity changes of the selected VOCs with storage time in the Tedlar bags, with some volatiles showing increasing signal intensity during Tedlar bag storage. Our results highlight the importance of careful design and implementation of experiments and clinical protocols to obtain relevant and reliable results.

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Pilot Study for Validation and Differentiation of Alveolar and Esophageal Air

Cited by 7 publications

References 12 publications

Ion Mobility Spectrometry Towards Environmental Volatile Organic Compounds Identification and Quantification: a Comparative Overview over Infrared Spectroscopy

Ion Mobility Spectrometry Towards Environmental Volatile Organic Compounds Identification and Quantification: a Comparative Overview over Infrared Spectroscopy

Diagnosis of Carcinogenic Pathologies through Breath Biomarkers: Present and Future Trends

Impact of breath sample collection method and length of storage of breath samples in Tedlar bags on the level of selected volatiles assessed using gas chromatography-ion mobility spectrometry (GC-IMS)

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