Pilot study of loss of the p53/p63 target gene <scp>PERP</scp> at the surgical margin as a potential predictor of local relapse in head and neck squamous cell carcinoma

Holmes, Brittany J.; Eyben, Rie von; Attardi, Laura D.; Kong, Christina S.; Le, Quynh‐Thu; Nathan, Cherie‐Ann O.

doi:10.1002/hed.26358

Cited by 6 publications

(4 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 33 However, other studies have suggested that PERP inhibits tumour growth and progression by promoting apoptosis. 34 , 35 In our study, meta-analysis of the LCE database showed increased PERP expression in lung adenocarcinoma tissues compared to paracancerous tissues. In a multivariate logistic regression, the level of PERP expression was correlated with the T and N phases of development.…”

Section: Discussionsupporting

confidence: 53%

PERP May Affect the Prognosis of Lung Adenocarcinoma by Inhibiting Apoptosis

Liu,

Han,

Luo

et al. 2024

CMAR

View full text Add to dashboard Cite

Background PERP, a member of the peripheral myelin protein gene family, is a new therapeutic target in cancer. The relationships between PERP and immune cell infiltration in lung cancer have not been studied. Therefore, the role of PERP in the tumour microenvironment (TME) of lung cancer needs to be further explored. Methods In this study, we explored the association between PERP expression and clinical characteristics by analysing data from the TCGA database. Cox regression and Kaplan‒Meier methods were used to investigate the relationship between the expression of PERP and overall survival in patients with lung adenocarcinoma (LUAD). The relationship between PERP expression and the degree of infiltration of specific immune cell subsets in LUAD was evaluated using the TIMER database and GEPIA. We also performed GO enrichment analysis and KEGG enrichment analysis to reveal genes coexpressed with PERP using the Coexpedia database. Finally, we verified the expression and function of PERP in LUAD tissues and the A549 cell line by RT‒PCR, Western blot, CCK-8, IHC, and wound healing assays. The mouse model was used to study the in vivo effects of PERP. Results According to our results, PERP expression was significantly higher in LUAD tissues and associated with the clinical characteristics of the disease. Survival was independently associated with PERP in LUAD patients. We further verified that PERP might regulate B-cell infiltration in LUAD to affect the prognosis of LUAD. To identify PERP-related signalling pathways in LUAD, we performed a genome-aggregation analysis (GSEA) between low and high PERP expression datasets. LUAD cells express higher levels of PERP than paracarcinoma cells, and PERP inhibits the proliferation and metastasis of A549 cells through apoptosis. Conclusion PERP may affect the prognosis of lung adenocarcinoma by inhibiting apoptosis and is associated with immune cell infiltration.

show abstract

Section: Discussionsupporting

confidence: 53%

PERP May Affect the Prognosis of Lung Adenocarcinoma by Inhibiting Apoptosis

Liu,

Han,

Luo

et al. 2024

CMAR

View full text Add to dashboard Cite

show abstract

“…Contemporary research on the molecular margin of HNSCC is progressively expanding, yielding promising biomarkers that are detailed in Table 3 79 , 232 – 237 . This burgeoning field of study underscores the potential of molecular analysis in enhancing the precision of surgical interventions for HNSCC.…”

Section: Assessment Of Head and Neck Squamous Cell Carcinoma Marginsmentioning

confidence: 99%

Surgical margins in head and neck squamous cell carcinoma: a narrative review

Chen,

Zhong,

Cao

et al. 2024

International Journal of Surgery

View full text Add to dashboard Cite

Head and neck squamous cell carcinoma (HNSCC), a prevalent and frequently recurring malignancy, often necessitates surgical intervention. The surgical margin (SM) plays a pivotal role in determining the postoperative treatment strategy and prognostic evaluation of HNSCC. Nonetheless, the process of clinical appraisal and assessment of the SMs remains a complex and indeterminate endeavor, thereby leading to potential difficulties for surgeons in defining the extent of resection. In this regard, we undertake a comprehensive review of the suggested surgical distance in varying circumstances, diverse methods of margin evaluation, and the delicate balance that must be maintained between tissue resection and preservation in head and neck surgical procedures. This review is intended to provide surgeons with pragmatic guidance in selecting the most suitable resection techniques, and in improving patients’ quality of life by achieving optimal functional and aesthetic restoration.

show abstract

“…Holmes et al analyzed the 2-year cumulative incidence of local recurrence in 44 patients with SCC and described 44.4% recurrence for the PERP-negative and 16.4% for the PERP-positive group. The authors highlighted that PERP loss at SCC surgical margins is a predictor of relapses (2). Overexpression of kallikrein 7 (KLK7) in oral SCC can promote degradation of desmosomes, favoring local tumor invasion and metastases to the lymph nodes (3).…”

Section: Dear Editormentioning

confidence: 99%