Pilot Study of the Correlation of Multiphoton Tomography of Ex Vivo Human Testis with Histology

Najari, Bobby B.; Ramasamy, Ranjith; Sterling, Joshua; Aggarwal, Amit; Sheth, Seema; Li, Philip S.; Dubin, Justin M.; Goldenberg, Simon; Jain, Manu; Robinson, Brian D.; Shevchuk, Maria M.; Scherr, Douglas S.; Goldstein, Marc; Mukherjee, Sushmita

doi:10.1016/j.juro.2012.03.124

Cited by 40 publications

(30 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…9 confocal fluorescence microscopy 10,11 and multiphoton tomography. 12 The spatial resolution, penetration depth and need for extrinsic labels or dyes, such as sperm-specific antibodies, vary greatly between these imaging modalities and might limit their use in clinical practice. The potential damage to spermatozoa or testicular tissue caused by these different modalities depends on the energy levels needed for molecular excitation, as well as the type and application of extrinsic labels, among other factors.…”

Section: Resultsmentioning

confidence: 99%

No relationship between biopsy sites near the main testicular vessels or rete testis and successful sperm retrieval using conventional or microdissection biopsies in 220 non-obstructive azoospermic men

Schwarzer¹,

Steinfatt²,

Schleyer³

et al. 2013

Asian J Androl

View full text Add to dashboard Cite

In 220 consecutive patients with non-obstructive azoospermia, sperm retrieval was attempted by a combination of conventional and microdissection testicular sperm extraction (TESE). For sperm retrieval, 2-3 conventional biopsies were performed followed by a microdissection TESE in cases of negative conventional biopsies. During the surgery, the vasculature of the testis was assessed using the operative microscope, and the location of positive biopsies was registered in relation to the blood supply. The overall sperm retrieval rate was 58.2%. From the initial conventional biopsies, sperm could be retrieved in 46.8% of the patients. With microdissection TESE, sperm could be retrieved from an additional 11.4% of the patients. The further use of microdissection TESE improved the sperm retrieval rate significantly (P50.017). No significant accumulation of positive biopsies was found towards the rete testis or the main testicular vessels.

show abstract

Section: Resultsmentioning

confidence: 99%

No relationship between biopsy sites near the main testicular vessels or rete testis and successful sperm retrieval using conventional or microdissection biopsies in 220 non-obstructive azoospermic men

Schwarzer¹,

Steinfatt²,

Schleyer³

et al. 2013

Asian J Androl

View full text Add to dashboard Cite

show abstract

“…MPM is a novel imaging modality that has been investigated for ex-vivo analysis of fresh tumor specimens from the human bladder [18], prostate [19] and the testis [8]. It also has been described for imaging lung [20] and gastrointestinal [21] tissues.…”

Section: Discussionmentioning

confidence: 99%

“…It captures intrinsic tissue signals based on autofluorescence and Second Harmonic Generation (SHG) from tissue components such as collagen, and when color-coded and overlaid, can provide images with information content similar to gold standard hematoxylin and eosin (H&E) histology [6–8]. …”

mentioning

confidence: 99%

Multiphoton microscopy to identify and characterize the transition zone in a mouse model of Hirschsprung disease

Aggarwal

Jain

Frykman

et al. 2013

Journal of Pediatric Surgery

Self Cite

View full text Add to dashboard Cite

Background The distribution of ganglion cells in the transition zone of Hirschsprung Disease (HD) colons is extremely variable. Determining the resection margin based on intraoperative biopsies may be imprecise. Multiphoton microscopy (MPM) is a novel imaging technology with the ability to visualize tissues in real time. In this study, we evaluate the potential of MPM to quantify ganglion cells in a murine model of HD. Methods After IACUC approval, formalin-fixed colons from 7 wild type (WT) and 6 Endothelin Receptor B gene (EdnrB) homozygous knockout (KO) mice with distal colonic aganglionosis were assessed by MPM for the presence of myenteric ganglion cells. MPM images were captured starting from the anus progressing proximally at 5 mm intervals. Hematoxylin and eosin (H&E) stained biopsies of the imaged were correlated with MPM findings. Results WT specimens showed normal myenteric plexus ganglia throughout the examined colon. In contrast, distal colons of EdnrB KO animals were devoid of ganglia up to 10 mm from the anus. Ganglion cells were visible starting at 20–30 mm proximal to the anus. The density of ganglion cells seen by MPM and histology correlated well. Conclusions MPM can clearly identify the myenteric plexus ganglia in both WT and KO mouse colons. Comparison with the H&E-stained sections showed reproducible correlation. MPM-based real-time imaging of the myenteric plexus may become a useful intraoperative decision-making tool in the future.

show abstract

“…Multiphoton microscopy has been applied successfully to differentiate normal from abnormal spermatogenesis in an ex vivo rodent model, and encouraging preliminary results have been reported in humans. [4748] Confocal fluorescence microscopy has also been used in a murine model of microdissection TESE. [49] Although the latter offers the advantage of rapid in vivo detection of sperm in the ST, the use of fluorescein to label sperm may limit the translation of this method to the clinical setting.…”

Section: Discussionmentioning

confidence: 99%

Micro-dissection testicular sperm extraction as an alternative for sperm acquisition in the most difficult cases of Azoospermia: Technique and preliminary results in India

Ashraf¹,

Singh²,

Raj³

et al. 2013

J Hum Reprod Sci

View full text Add to dashboard Cite

CONTEXT:Non-obstructive azoospermia (NOA) is an unfavorable prognostic condition for male infertility since spermatogenesis is disrupted. Sperm retrieval (SR) coupled with intracytoplasmic sperm injection (ICSI) is the only option for men with NOA who seek fertility. Among the SR techniques, microdissection testicular sperm extraction (micro-TESE) has been applied with encouraging results.AIMS:We describe how we implemented the micro-TESE procedure and present initial micro-TESE experience in a group of men with NOA and poor prognosis for SR.SETTINGS AND DESIGN:Case series of men with NOA treated in a tertiary healthcare center.MATERIALS AND METHODS:An Assisted Reproductive Technology (ART) facility was setup to perform SR using microsurgery. Fourteen men with NOA and previous failed retrievals or unfavorable histologic results underwent micro-TESE while their female partners received ovarian stimulation for oocyte pickup (OCP). Micro-TESE was performed the day prior to OCP and testicular sperm were used for sperm injections. We assessed retrieval rates and ICSI outcomes.STATISTICAL ANALYSIS:Outcomes of SR and ICSI were analyzed descriptively. Mann-Whitney and Fisher exact test were used to compare characteristics of men with successful and failed SR.RESULTS:The success of micro-TESE was 50.0% with no major complications. A clear microscopic distinction between enlarged and collapsed seminiferous tubules was seen in 35.7% of the cases, and sperm were retrieved in all but one of these cases. Patients with successful and failed retrieval did not differ with respect to baseline characteristics, use of medical therapy, presence of varicocele, and testicular histology. Sperm injections resulted in normal fertilization and embryo cleavage of 64% and 75%, respectively. A total of five transfers with an average of 1.5 embryos resulted in a cumulative clinical pregnancy rate per ICSI cycle of 28.6%, with an implantation rate of 33.3%.CONCLUSIONS:We were successful in integrating the micro-TESE procedures to the in vitro fertilization (IVF) laboratory. Our initial experience with micro-TESE applied to the most difficult cases of azoospermia is reassuring.

show abstract

Pilot Study of the Correlation of Multiphoton Tomography of Ex Vivo Human Testis with Histology

Cited by 40 publications

References 30 publications

No relationship between biopsy sites near the main testicular vessels or rete testis and successful sperm retrieval using conventional or microdissection biopsies in 220 non-obstructive azoospermic men

No relationship between biopsy sites near the main testicular vessels or rete testis and successful sperm retrieval using conventional or microdissection biopsies in 220 non-obstructive azoospermic men

Multiphoton microscopy to identify and characterize the transition zone in a mouse model of Hirschsprung disease

Micro-dissection testicular sperm extraction as an alternative for sperm acquisition in the most difficult cases of Azoospermia: Technique and preliminary results in India

Contact Info

Product

Resources

About