Pilot study on the time course of apoptosis in the regenerating corneal epithelium

Estil, Svein; Kravik, Katherine; Haaskjold, Erling; Refsum, Sigvald; Bjerknes, Rolf; Wilson, Graeme

doi:10.1034/j.1600-0420.2002.800511.x

Cited by 6 publications

(4 citation statements)

References 23 publications

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“…This was supported by Estil et al [22] who considered that damaged cells undergo apoptosis and are soon shed into the tear film. While on day three, it showed epithelium which completely filled the gap, but revealed superficial keratinization, seen as lost or ghost nuclei.…”

Section: Discussionsupporting

confidence: 57%

Role of vitamin A in the healing process of alkali caused corneal injury of adult male albino rat: Histological and immunohistochemical study

Abdelwahab

El-Hameed

Saber

et al. 2017

Journal of Medical Histology

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Background and Aim: Alkali burn of the cornea is considered as the most dangerous injury to the eye. It causes corneal infection, ulceration, perforation, neovascularization (NV) and opacification. Vitamin A is necessary for the normal growth and differentiation of epithelium; it is expected to promote the mechanical repair of corneal epithelial defects The present study is planned to investigate the effect of vitamin A eyegel on wound healing of the corneal alkali burn in rats. Materials and Methods: A total of thirty male albino rats were used. Rats were divided randomly into four groups: Group I, the control group; included three rats, received distilled water. Each of group II, III, IV included 9 rats: their central corneas of the right eyes were injured by contacting them with filter paper saturated with 0.01 m NaOH for 45 seconds. Group II, the non-treated group, injured and received distilled water. Group III, the antibiotic treated group, received antibiotic eye drops (lincomycin hydrochloride eye drops) 3 times per day for 3 days. Group IV, vitamin A treated group, received the same course of antibiotic and vitamin A eye gel (Hypotear gel 1000 IU/g) three times per day. for 3 days. The eyeball was taken out, rapidly fixed and processed for light microscopic, immunohistochemical and morphometric studies. Specimens were taken after 24, 48 and 72 hr. post-injury for tissue preparation and study of structural changes and immuno-histochemical analysis using Ki67 (detection of cellular proliferation) and transforming growth factor -beta (TGF-β) which is one of the most critical growth factors in establishing the pathologic lesion after corneal alkali burn. Results: Hematoxylin and eosin stained sections showed rapid healing of corneal ulcer in vitamin A treated group, with absence of neo-vasculariztion and inflammatory cellular infiltration. Immunohistochemical results showed that the use of vitamin A enhance cell proliferation (detected with Ki67) and decrease the expression of TGF-β which is one of the most critical growth factors in establishing the pathological lesion after corneal alkali burn. Conclusions: Vitamin A eye gel helped rapid healing of corneal alkali burn. This effect may be due to its anti-inflammatory effect and stimulation of cell proliferation.

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Section: Discussionsupporting

confidence: 57%

Role of vitamin A in the healing process of alkali caused corneal injury of adult male albino rat: Histological and immunohistochemical study

Abdelwahab

El-Hameed

Saber

et al. 2017

Journal of Medical Histology

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“…We previously demonstrated and characterized p53 expression in the rodent corneal epithelium (18)(19)(20). Others have found that UV irradiation was able to induce the p53 protein (8) and p53-dependent apoptosis in the corneal epithelium (21)(22)(23). However, its causative contribution to apoptosis has yet to be determined.…”

Section: Introductionmentioning

confidence: 99%

p53 protein subcellular localization and apoptosis in rodent corneal epithelium cell culture following ultraviolet irradiation

Tendler

Pokroy

Panshin³

et al. 2013

International Journal of Molecular Medicine

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The tumor-suppressor gene p53 encodes a phosphoprotein involved in the control of cell growth. p53 expression and function have been documented in malignancy, apoptosis and the aging processes. Recently, p53 has been mapped and characterized in the normal cornea across different species. In the present study, high levels of cytoplasmic p53 protein were noted in normal primary corneal epithelium cultures by immunohistochemistry and western blot analysis. Following ultraviolet (UV) irradiation, the level of cytoplasmic p53 protein expression was increased beginning from 30 min and lasting until 6 h post-irradiation and then returned close to control levels by 24 h. Cytoplasmic p53 phosphorylation was detected from 30 min following UV treatment until 6 h post-irradiation. p53 protein became apparent in the nucleus in a fraction of these cultured cells beginning 30 min following UV irradiation and was still present 24 h later. We also found that p53 colocalized with mitochondria 2 h following UV irradiation in some of the cells and remained there up to 24 h. As the expression levels of p53 transcription following UV irradiation were not significantly altered, the increase in cytoplasmic p53 protein expression may be conditional only upon post-translational stabilization. We also observed that the apoptotic index increased following UV irradiation in the same time frame as the p53 nuclear transfer and was partially suppressed by pifithrin-α, which is a reversible inhibitor of p53-mediated apoptosis and p53-dependent gene transcription. The present study offers new evidence suggesting that cytoplasmic p53 in rodent corneal epithelium is functionally active.

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“…Ezt kö veti a migrációs fázis, amely monolayer sejtréteg kialaku lásával és sejtkapcsoló elemek képződésével jár [17]. A corneaeróziók utáni hámgyógyulást vizsgálva megálla pították, hogy a szimmetrikusan létrejövő kétoldali hám záródás sebessége a sebszél minden pontján egyformá nak tekinthető [15,18]. A folyamat végén a proliferációs fázisban épül ki a végleges hámvastagság.…”

Section: Megbeszélésunclassified

Crosslinking kezelés hatása a szaruhártya hámosodására

et al. 2017

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Bevezetés és célkitűzés:A crosslinking kezelés szaruhártyahámosodásra gyakorolt hatásának vizsgálata. Betegek és mód-szer: Vizsgálatunkba 39 keratoconusos beteget vontunk be crosslinking kezelés után, és 11 pácienst fotorefraktív keratectomiát követően (kontroll) crosslinking kezelést követően 21, 45 és 69 órával. A festődő szaruhártyahámhi ány területét a fotorefraktív keratectomiát követően 16, 37 és 61 órával meghatároztuk. A fotorefraktív keratectomia kezelések utáni kontrollok időpontja nem egyezett a crosslinking kezelést követő kontrollidőpontokkal, így a fotoref raktív keratectomiás csoportnál a hámhiány feltehető méretét a crosslinking kezelési csoport mérési időpontjaira számítottuk, és χ 2 teszt segítségével hasonlítottuk össze a két csoportban a hámhiány méretét. Eredmények: A cornea crosslinking kezelést követően a szaruhártya hámjának záródása a kezeléstől számított három napon belül, fotorefrak tív keratectomiát követően négy napon belül következett be. 45 és 69 óra elteltével a két csoport között szignifikáns különbség igazolódott a hámhiány méretében (p = 0,005 és p<0,001). Következtetés: A crosslinking kezelést követő en a szaruhártya hámosodása gyorsabb, mint fotorefraktív keratectomiát követően. Vizsgálatunk a crosslinking keze lés hámgyógyulásra kifejtett kedvező hatását igazolta. Orv. Hetil., 2017, 158(10), 376-379. Kulcsszavak: crosslinking, fotorefraktív keratectomia, hámgyógyulás Impact of crosslinking on corneal epithelial healingIntroduction and aim: To analyse the effect of crosslinking (CXL) on corneal epithelial healing. Patients and method: 39 keratoconus patients following CXL and 11 patients following PRK were enrolled in this study. 21, 45 and 69 hours after CXL and 16, 37 and 61 hours following PRK slitlamp photographs were taken using fluorescein staining and the size of the epithelial defect and the time of epithelial closure was determined. As the observation timepoints differed in both groups, the size of the epithelial defect following PRK was calculated for the time points used for analysis of CXL patients. These data were compared using chiquadrat test. Results: Following crosslinking, epithe lial closure occured within 3, following PRK within 4 days. In the area of epithelial erosion 45 and 69 hours after CXL or PRK treatment statistically significant difference (p = 0.005 and p<0.001) was verified. Conclusion: Our re sults show beneficial effect of crosslinking on corneal epithelial healing.

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Pilot study on the time course of apoptosis in the regenerating corneal epithelium

Cited by 6 publications

References 23 publications

Role of vitamin A in the healing process of alkali caused corneal injury of adult male albino rat: Histological and immunohistochemical study

Role of vitamin A in the healing process of alkali caused corneal injury of adult male albino rat: Histological and immunohistochemical study

p53 protein subcellular localization and apoptosis in rodent corneal epithelium cell culture following ultraviolet irradiation

Crosslinking kezelés hatása a szaruhártya hámosodására

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