PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in
            <i>Arabidopsis thaliana</i>

Feraru, Elena; Feraru, Mugurel I.; Barbez, Elke; Waidmann, Sascha; Sun, Lin; Gaidora, Angelika; Kleine‐Vehn, Jürgen

doi:10.1073/pnas.1814015116

Cited by 98 publications

(119 citation statements)

References 33 publications

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“…This R2D2 (ratiometric version of two D2s) system for rapid, sensitive and high-resolution detection of auxin sensing activities in living Arabidopsis tissues is now widely used (e.g. Caggiano et al, 2017;Di Mambro et al, 2017;Larsson et al, 2017;Liu et al, 2017;Bhatia et al, 2019;Feraru et al, 2019;Radoeva et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Minimal auxin sensing levels in vegetative moss stem cells revealed by a ratiometric reporter

2019

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Summary Efforts to reveal ancestral functions of auxin, a key regulator of plant growth and development, and its importance for evolution have been hampered by a fragmented picture of auxin response domains in early‐diverging land plants. We report the mapping of auxin sensing and responses during vegetative moss development using novel reporters. We established a moss‐specific ratiometric reporter (PpR2D2) for Auxin Response Element‐ and AUXIN RESPONSE FACTOR‐independent auxin sensing in Physcomitrella patens, and its readout during vegetative development was compared with new promoter‐based GmGH3::GFPGUS and DR5revV2::GFPGUS auxin response reporters. The ratiometric reporter responds rapidly to auxin in a time‐, dose‐ and TRANSPORT INHIBITOR RESISTANT1/AUXIN F‐BOX‐dependent manner and marks known, anticipated and novel auxin sensing domains. It reveals proximal auxin sensing maxima in filamentous tissues and sensing minima in all five vegetative gametophytic stem cell types as well as dividing cells. PpR2D2 readout is compliant with an ancestral function of auxin as a positive regulator of differentiation vs proliferation in stem cell regions. The PpR2D2 reporter is a sensitive tool for high‐resolution mapping of auxin sensing, which can increase our knowledge of auxin function in early‐diverging land plants substantially, thereby advancing our understanding of its importance for plant evolution.

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Section: Introductionmentioning

confidence: 99%

Minimal auxin sensing levels in vegetative moss stem cells revealed by a ratiometric reporter

2019

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“…PILS transcription is highly sensitive to environmental fluctuations, such as light and temperature, integrating external signals to modulate auxin-dependent growth rates (Beziat et al, 2017;Feraru et al, 2019). Using a forward genetic screen, we reveal here that PILS genes also function as important integrators of endogenous cues, such as brassinosteroid (BR) hormone signaling.…”

Section: Introductionmentioning

confidence: 85%

“…Next, we assessed whether BR signaling may also regulate PILS protein turnover, using lines that constitutively overexpress PILS-GFP proteins, under the control of the CaMV The dual effect of BR signaling on PILS transcription and PILS protein abundance is reminiscent to the impact of high temperature, which also represses PILSes in a transcriptional and posttranslational manner (Feraru et al, 2019). High temperature-induced downregulation of PILS abundance elevates nuclear auxin input and increases root organ length (Feraru et al 2019). Notably, BRI1-dependent BR signaling is also implied in root growth promotion under elevated ambient temperature (Martins et al, 2017).…”

Section: Br Signaling Modulates Pils Gene Expression and Pils Proteinmentioning

confidence: 99%

“…Despite some structural similarities, the evolution of PIN and PILS proteins within the plant lineage is distinct (Barbez et al, 2012;Feraru et al, 2012). At the subcellular level, PILS putative auxin facilitators control the intracellular auxin accumulation at the ER and restrict nuclear availability and signaling of auxin (Barbez et al, 2012;Feraru et al, 2012;Beziat et al, 2017;Feraru et al, 2019). Thereby, PILS proteins determine the cellular sensitivity to auxin and contribute to various growth processes during plant development (Barbez et al, 2012;Beziat et al, 2017;Feraru et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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PIN-LIKES coordinate brassinosteroid signalling with nuclear auxin input in Arabidopsis thaliana

Sun¹,

Feraru²,

Feraru³

et al. 2019

Preprint

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Auxin and brassinosteroids (BR) are crucial growth regulators and display overlapping functions during plant development. Here, we reveal an alternative phytohormone crosstalk mechanism, revealing that brassinosteroid signaling controls nuclear abundance of auxin. We performed a forward genetic screen for imperial pils (imp) mutants that enhance the overexpression phenotypes of PIN-LIKES (PILS) putative intracellular auxin transport facilitator. Here we report that the imp1 mutant is defective in the brassinosteroid-receptor BRI1. BR signaling transcriptionally and posttranslationally represses accumulation of PILS proteins at the endoplasmic reticulum, thereby increasing nuclear abundance and signaling of auxin. We demonstrate that this alternative phytohormonal crosstalk mechanism integrates BR signaling into auxin-dependent organ growth rates and likely has widespread importance for plant development.

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“…YUC6 induction increased the expression of several XTH genes, whereas GH3.6 expression had a comparatively weaker impact (SFig.2). Similarly, kynurenin-induced, pharmacological inhibition of auxin biosynthesis (He et al, 2011) and PIN-like (PILS) intracellular auxin transport-induced repression of nuclear auxin signalling Béziat et al, 2017;Feraru et al, 2019) exerted a weak effect on XTH genes (Fig.1C).…”

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confidence: 89%

Xyloglucan remodelling defines differential tissue expansion in plants

Velasquez

Gallemí

Aryal

et al. 2019

Preprint

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Size control is a fundamental question in biology, showing incremental complexity in caseof cell wall surrounded plant cells. Here we show that auxin signalling restricts the complexity of extracellular xyloglucans, which defines cell wall properties and tissue expansion. Our work uncovers an alternative mechanism of how the phytohormone auxin modulates the cell wall for steering differential growth control in gravitropic hypocotyls.The phytohormone auxin has outstanding importance for plant growth and development.Despite its significance, we just start to understand the subcellular mechanisms by which auxin exerts growth control of a cell wall-encapsulated plant cell. In a concentration-and cell-type-dependent manner, auxin signalling steers promotion and repression of cell expansion (Sauer et al., 2013). These cellular levels of auxin rely on a complex interplay between its metabolism and intercellular transport Sauer and Kleine-Vehn, 2019). On the other hand, tissue specific expression of auxin signalling components

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PILS6 is a temperature-sensitive regulator of nuclear auxin input and organ growth in Arabidopsis thaliana

Cited by 98 publications

References 33 publications

Minimal auxin sensing levels in vegetative moss stem cells revealed by a ratiometric reporter

Minimal auxin sensing levels in vegetative moss stem cells revealed by a ratiometric reporter

PIN-LIKES coordinate brassinosteroid signalling with nuclear auxin input in Arabidopsis thaliana

Xyloglucan remodelling defines differential tissue expansion in plants

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