PIM-Related Kinases Selectively Regulate Olfactory Sensations in<i>Caenorhabditis elegans</i>

Kalichamy, Karunambigai; Ikkala, Kaisa; Pörsti, Jonna; Santio, Niina M.; Tuomaala, Joel; Jha, Sweta; Holmberg, Carina I.; Koskinen, Päivi J.

doi:10.1523/eneuro.0003-19.2019

Cited by 3 publications

(3 citation statements)

References 31 publications

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“…The C. elegans strains were grown and maintained at 20 °C on NGM (nematode growth medium) agar plates seeded with the E. coli strain OP50 or its streptomycin-resistant derivative OP50-1 using standard culturing methods [ 57 , 58 ]. Before experimentation, the C. elegans populations were synchronized by bleaching.…”

Section: Methodsmentioning

confidence: 99%

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Biomonitoring of Indoor Air Fungal or Chemical Toxins with Caenorhabditis elegans nematodes

et al. 2023

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Bad indoor air quality due to toxins and other impurities can have a negative impact on human well-being, working capacity and health. Therefore, reliable methods to monitor the health risks associated with exposure to hazardous indoor air agents are needed. Here, we have used transgenic Caenorhabditis elegans nematode strains carrying stress-responsive fluorescent reporters and evaluated their ability to sense fungal or chemical toxins, especially those that are present in moisture-damaged buildings. Liquid-based or airborne exposure of nematodes to mycotoxins, chemical agents or damaged building materials reproducibly resulted in time- and dose-dependent fluorescent responses, which could be quantitated by either microscopy or spectrometry. Thus, the C. elegans nematodes present an easy, ethically acceptable and comprehensive in vivo model system to monitor the response of multicellular organisms to indoor air toxicity.

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Section: Methodsmentioning

confidence: 99%

“…To determine whether volatile compounds were attractive or aversive to the C. elegans nematodes, chemotactic assays were performed as described previously [ 35 , 58 ]. Briefly, 50–100 well-fed and washed young adults were placed in the middle of an NMG agar plate without food bacteria.…”

Section: Methodsmentioning

confidence: 99%

Biomonitoring of Indoor Air Fungal or Chemical Toxins with Caenorhabditis elegans nematodes

et al. 2023

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“…In this study, we have identified the CP alpha 1, alpha 2 and beta 2 subunits as novel substrates for the oncogenic PIM1 kinase. The three serine/threonine-specific PIM family kinases regulate cell growth, metabolism, and motility, and have also neuronal functions [23][24][25][26]. They have been implicated in both hematopoietic malignancies and solid cancers [23,27,28], and are therefore promising targets for cancer therapy.…”

Section: Introductionmentioning

confidence: 99%

PIM1 accelerates prostate cancer cell motility by phosphorylating actin capping proteins

et al. 2020

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Background: The PIM family kinases promote cancer cell survival and motility as well as metastatic growth in various types of cancer. We have previously identified several PIM substrates, which support cancer cell migration and invasiveness. However, none of them are known to regulate cellular movements by directly interacting with the actin cytoskeleton. Here we have studied the phosphorylation-dependent effects of PIM1 on actin capping proteins, which bind as heterodimers to the fast-growing actin filament ends and stabilize them. Methods: Based on a phosphoproteomics screen for novel PIM substrates, we have used kinase assays and fluorescence-based imaging techniques to validate actin capping proteins as PIM1 substrates and interaction partners. We have analysed the functional consequences of capping protein phosphorylation on cell migration and adhesion by using wound healing and real-time impedance-based assays. We have also investigated phosphorylation-dependent effects on actin polymerization by analysing the protective role of capping protein phosphomutants in actin disassembly assays. Results: We have identified capping proteins CAPZA1 and CAPZB2 as PIM1 substrates, and shown that phosphorylation of either of them leads to increased adhesion and migration of human prostate cancer cells. Phosphorylation also reduces the ability of the capping proteins to protect polymerized actin from disassembly. Conclusions: Our data suggest that PIM kinases are able to induce changes in actin dynamics to support cell adhesion and movement. Thus, we have identified a novel mechanism through which PIM kinases enhance motility and metastatic behaviour of cancer cells.

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A systematic review on active sites and functions of PIM-1 protein

et al. 2022

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PIM-Related Kinases Selectively Regulate Olfactory Sensations inCaenorhabditis elegans

Abstract: Visual Abstract

Cited by 3 publications

References 31 publications

Biomonitoring of Indoor Air Fungal or Chemical Toxins with Caenorhabditis elegans nematodes

Biomonitoring of Indoor Air Fungal or Chemical Toxins with Caenorhabditis elegans nematodes

PIM1 accelerates prostate cancer cell motility by phosphorylating actin capping proteins

A systematic review on active sites and functions of PIM-1 protein

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