Pine needle oil induces G2/M arrest of HepG2 cells by activating the ATM pathway

Shan

Journal Cellular Physiology

et al. 2018

Acquired resistance to cytotoxic antineoplastic agents is a major clinical challenge in tumor therapy; however, the mechanisms involved are still poorly understood. In this study, we show that knockdown of CtIP, a corepressor of CtBP, promotes cell proliferation and alleviates G2/M phase arrest in etoposide (Eto)‐treated HCT116 cells. Although the expression of p21 and growth arrest and DNA damage inducible α (GADD45a), which are important targets of p53, was downregulated in CtIP‐deficient HCT116 cells, p53 deletion did not affect G2/M arrest after Eto treatment. In addition, the phosphorylation levels of Ser317 and Ser345 in Chk1 and of Ser216 in CDC25C were lower in CtIP‐deficient HCT116 cells than in control cells after Eto treatment. Our results indicate that CtIP may enhance cell sensitivity to Eto by promoting G2/M phase arrest, mainly through the ATR‐Chk1‐CDC25C pathway rather than the p53‐p21/GADD45a pathway. The expression of CtIP may be a useful biomarker for predicting the drug sensitivity of colorectal cancer cells.

Section: Resultsmentioning

confidence: 99%

“…G1/S and G2/M are two major cell-cycle checkpoints that ensure proper cell-cycle progression. G2/M arrest is caused by DNA damage and is also regulated by DNA damage repairrelated proteins, such as ataxia-telangiectasia mutated (ATM) (Hua et al, 2013;Qiu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

CtIP promotes G2/M arrest in etoposide‐treated HCT116 cells in a p53‐independent manner

Shan

Journal Cellular Physiology

et al. 2018

Environmental Toxicology

“…Previous reports have demonstrated the apoptotic effects of TW oil on HepG2 liver cancer cell lines 22 . Further studies have shown that TW oil facilitates cell cycle arrest in HepG2 cells 23 . Chrysin is a biologically active dietary flavonoid in many plants and has been known to be the most active bioactive component in TW extract and has been known to induce apoptosis and suppress autophagic clearance 16,24,25 .…”

Section: Discussionmentioning

confidence: 99%

“…22 Further studies have shown that TW oil facilitates cell cycle arrest in HepG2 cells. 23 Chrysin is a biologically active dietary flavonoid in many plants and has been known to be the most active bioactive component in TW extract and has been known to F I G U R E 4 Effect of P. morrisonicola Hayata extract (PE) on apoptosis: (A) Flow cytometry analysis of HA22T cells show increase in apoptotic cell numbers upon PE treatment. PE showed better effect than chrysin.…”

Section: Pe Downregulates Autophagic Proteins That Were Correlated Wi...mentioning

confidence: 99%

Principle active metabolites of Pinus morrisonicolaHayata synergistically inhibit cell proliferation and autophagy to elevate apoptosis in hepatocellular carcinoma cells

Shibu,

Chen,

Yang

et al. 2023

Hepatocellular carcinoma (HCC), a common primary tumor of liver is a leading cause of cancer‐associated deaths. Improving cellular apoptosis and enhancing autophagic clearance is been considered to improve treatment outcomes of HCC. Polyphenols from Pinus morrisonicola (Hayata) have shown various physiological and therapeutic benefits and the flavonoid chrysin is been known for their anticancer effects. However, the main bioactive principle and the mechanism underlying the antitumor activity of pine needle extract are not clear yet. In this study, the effects of ethanol extract from pine needle on HCC cells were determined. The results show that when compared with administration of chrysin alone, a fraction containing pinocembrin, chrysin, and tiliroside significantly reduced autophagy and increased apoptosis. The results also correlated with decrease in cell cycle regulators and the autophagic proteins like LC3‐II. Collectively, the results imply the fraction containing pinocembrin, chrysin, and tiliroside as an ideal complementary medicine for an effective antitumor activity.

“…23,24 Pine needle oil treatment arrested the Hepg2 cycle cell and inhibited the proliferation of liver cancer cell line. 25 Other parts of the tree possess remarkable antioxidant, anti-inflammatory and antimicrobial activities. 26,27 Plant-derived products with a wide range of phytochemicals and phenolic compounds have considerable as an antioxidant, antiradical, antiinflammatory and anticarcinogenic activities.…”

Section: Discussionmentioning

confidence: 99%

Evacuation the Effect of Single- and Multi-dose Administration of Ethanolic Extract of Pinus eldarica Pollen Against Acetaminophen-induced Rat Liver Injury

Asadian

Moallem

Moshiri

et al. 2020

Int J Basic Sci Med

Introduction: Acetaminophen (APAP)-induced liver injury is one of the main causes of acute liver failure in the world. Pinus eldarica is specially distributed in the north of Iran and has been used for decades to treat wounds, pain, infection, fever, bronchitis and inflammation in many countries. This study was undertaken to evaluate the role of pine pollen extract (PE) on APAP-induced hepatotoxicity. Methods: This study was conducted in two separate parts: single- and multi- dose administration of PE. In a multiple dosing regimen, different doses of extract (10, 20, 30, 40 and 50 mg/kg) were orally administrated for 2 weeks and then on the 15th day, the animals received a single dose of APAP (600 mg/kg). In the second part, the highest dose of PE (50 mg/kg) was orally administrated half an hour after receiving the APAP at dose of 600 mg/kg. The negative and positive control groups were treated with normal saline and N-acetylcysteine (NAC), respectively. At the end of procedure, the biochemical parameters including alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) as well as pathological findings were evaluated. Results: A single and multiple oral dose of APAP increased the serum level of ALT, AST and ALP that were significantly attenuated by PE administration in both model. Indeed, exposure to APAP caused extensive necrosis and lymphocytic inflammation that were completely prevented by single- and multi-dose administration of PE, specially at a high dose. Conclusion: Finally, the present results indicate that PE could reverse the reduction of aminotransferases and improve histological changes of APAP-induced liver toxicity. Further research is needed to confirm this finding and reveal the exact mechanisms.