Pineal Gland Post‐natal Growth in Rat Revisited

Ferreira-Medeiros, Mildred; Mandarim-de-Lacerda, Carlos Alberto; Correa-Gillieron, Elenice Maria

doi:10.1111/j.1439-0264.2007.00763.x

Cited by 7 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Light and electron microscopy has allowed the fine description of the ontogenesis of the mammalian pineal gland (in rats 16,17 ; dogs 10,11 ; horses 18 ; sheep 14 ; and in humans 19 ). But these data are contradictory.…”

Section: Species-specific Features Of the Pineal Glands In Canidsmentioning

confidence: 99%

Pineal Gland Morphology in Relation to Age and Season in Three Canidae Species

2019

View full text Add to dashboard Cite

Introduction The aim of the present study was to determine the morphological features of the pineal gland in three closely related Canidae species (raccoon dog, Nyctereutes procyonoides Gray, 1834; silver fox, Vulpes vulpes L., 1758; and blue fox, Vulpes lagopus L., 1758) of different ages during the breeding (spring) and nonbreeding (winter) periods. Materials and Methods Histological analysis of the pineal glands of canids was performed. Results The morphological changes in the pineal gland detected in the current study are either age-associated, including increase in the reticular fibers and vascularization in the studied species, as well as increase in the amount of the protruding septae in the blue fox, or seasonally related, including an increase in the number and size of blood vessels. The present work reported two types of pigments: lipofuscin (primarily in the silver fox) and melanin (primarily in the raccoon dog and in the blue fox). The pineal gland in the blue fox is characterized by the ability to form corpora arenacea. Conclusions The present study provides the first insight into the morphological changes of the pineal gland in three closely related Canidae species of different ages during the breeding (spring) and nonbreeding (winter) periods, and showed some species-specific features of gland morphology. The aspects concerning the biogenesis of the calcium concretions and the factors influencing the accumulation of pigments need further investigation.

show abstract

Section: Species-specific Features Of the Pineal Glands In Canidsmentioning

confidence: 99%

Pineal Gland Morphology in Relation to Age and Season in Three Canidae Species

2019

View full text Add to dashboard Cite

show abstract

“…Stereology. An example of rat pineal gland and pinealocytes (based on Ferreira-Medeiros et al, 2007): A -Morphometry, a stage micrometer was used to measure larger diameters (D1), and smaller diameters (D2) of pinealocytes. BStereology, the two planes of a 'disector' (up and down) spaced apart by three micrometers are shown.…”

Section: Estimating a Volumementioning

confidence: 99%

Tips for Studies with Quantitative Morphology (Morphometry and Stereology)

Mandarim-de-Lacerda

Sol

2017

Int. J. Morphol.

View full text Add to dashboard Cite

MANDARIM-DE-LACERDA, C. A. & DEL SOL, M.Tips for studies with quantitative morphology (morphometry and stereology). Int. J. Morphol., 35(4):1482Morphol., 35(4): -1494Morphol., 35(4): , 2017. SUMMARY:The quantitative morphology contributes to making the studies less subjective and reproducible. Quantitative results are analyzed with statistics and should be based on a well-defined sample. Quantitative variables can be 'continuous' or 'discrete.' In this text, the term 'morphometry' is used to design a simpler procedure for measuring structures with a 'ruler.' The term 'stereology' is used in estimating quantities in the 3-dimensional space analyzing 2-D cut sections. Correction factors may be necessary for the retraction and compression of the tissues. In histological sections, the 'caps' of the objects that have been sectioned tangentially are lost when chemical agents remove the paraffin distorting the analysis. Moreover, the analyses based on digital images should consider the same format and the same size (pixels) to all pictures allowing a comparison between groups. Stereology can be 'model-based': points within a frame are counted to estimate the 'volume density' (Vv) of a structure, and intercepts are counted to assess the 'surface density' (Sv). Counting structures within a frame allow estimating the 'length density' (Lv). Newer and more complex 'design-based' procedures are considered unbiased. The key point is that design-based inference does not require assumptions about the material and uses the 'random sampling' approach. The estimation of the number of objects requires a 3-D (volume) probe and therefore the 'disector ' technique. This review aimed to contribute to the execution of the project, the correct sampling and the data obtained with morphometry and stereology.

show abstract