Pingyangmycin as a 99mTc carrier in tumor localization

Huang, Tiangui; Zhang, Yanhua; Wang, Jixin; Huang, Junxin; Liu, Dingxin; Mong, Shucong

doi:10.1016/0969-8051(94)00136-8

Nuclear Medicine and Biology

1995

DOI: 10.1016/0969-8051(94)00136-8

|View full text |Cite

Pingyangmycin as a 99mTc carrier in tumor localization

Tiangui Huang

Yanhua Zhang

Jixin Wang

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2008

2015

Publication Types

Select...

Article3

Relationship

Self Cite0

Independent3

Authors

Journals

Cited by 3 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Technetium-99m ( 99m Tc) is used in the medical field for diagnosis purposes with a large number of organic carriers. These include 99m Tc-mercaptoactyltriglycine, 99m Tc-gluconate, 99m Tc-dimercaptosuccinic acid, , 99m Tc-methylene-diphosphonate, 99m Tc-dicarboxypropane-diphosphonate, 99m Tc-ethylenediaminetetramethylene-phosphonate, and 99m Tc-pingyangmycin …”

mentioning

confidence: 99%

Liquid Scintillation Counting Methodology for ⁹⁹Tc Analysis: A Remedy for Radiopharmaceutical Waste

Khan

2015

Anal. Chem.

View full text Add to dashboard Cite

This paper presents a new approach for liquid scintillation counting (LSC) analysis of single-radionuclide samples containing appreciable organic or inorganic quench. This work offers better analytical results than existing LSC methods for technetium-99 ((99g)Tc) analysis with significant savings in analysis cost and time. The method was developed to quantify (99g)Tc in environmental liquid and urine samples using LSC. Method efficiency was measured in the presence of 1.9 to 11 900 ppm total dissolved solids. The resultant quench curve proved to be effective for quantifying spiked (99g)Tc activity in deionized water, tap water, groundwater, seawater, and urine samples. Counting efficiency was found to be 91.66% for Ultima Gold LLT (ULG-LLT) and Ultima Gold (ULG). Relative error in spiked (99g)Tc samples was ±3.98% in ULG and ULG-LLT cocktails. Minimum detectable activity was determined to be 25.3 and 22.7 mBq for ULG-LLT and ULG cocktails, respectively. A preconcentration factor of 1000 was achieved at 100 °C for 100% chemical recovery.

show abstract

mentioning

confidence: 99%

Liquid Scintillation Counting Methodology for ⁹⁹Tc Analysis: A Remedy for Radiopharmaceutical Waste

Khan

2015

Anal. Chem.

View full text Add to dashboard Cite

show abstract

Recombinant Human IL-24 Suppresses Lung Carcinoma Cell Growth via Induction of Cell Apoptosis and Inhibition of Tumor Angiogenesis

Xie

Sheng

Xiang

et al. 2008

Cancer Biotherapy and Radiopharmaceuticals

View full text Add to dashboard Cite

Previous studies have shown that interleukin-24 (IL-24; mda-7) as a novel tumor suppressor gene has tumor-suppressive activity against a broad spectrum of human cancers. However, the therapeutic effect of the recombinant human IL-24 (rhIL-24) protein purified from prokaryotic cells on human lung cancers has not been reported. In this study, we cloned the human gene coding for IL-24 from lipopolysaccharide-activated human peripheral blood mononuclear cells (PBMCs) by reverse-transcriptase polymerase chain reaction and constructed an expression vector pBV220-IL-24. We then transfected Escherichia coli DH5alpha with pBV220-IL-24. The soluble rhIL-24 was obtained from purified insoluble inclusion bodies of transfected cells by a denaturing and renaturing process. We demonstrated that the purified soluble rhIL-24 protein with 18.5 kappaDa was capable of (1) inducing in vitro apoptosis of A549 lung carcinoma cells; (2) activating PBMCs to secrete cytokines such as IL-6, tumor necrosis factor-alpha, and interferon-gamma; (3) inhibiting the formation of blood capillaries on chicken embryonic allantois and in vivo tumor angiogenesis; and (4) inhibiting A549 lung tumor cell growth in vitro and in vivo. Therefore, our results indicate its potent suppressive effect on human lung carcinoma cell line and warrant its further investigation for therapeutic application against human lung cancer.

show abstract

Recombinant Human Interleukin-24 Suppresses Gastric Carcinoma Cell GrowthIn VitroandIn Vivo

Zhang

Xie

et al. 2009

Cancer Investigation

View full text Add to dashboard Cite

Previous studies have demonstrated that interleukin-24 [IL-24; originally called melanoma differentiation associated gene-7 (mda-7)] as a novel tumor suppressor gene has tumor-suppressive activity against a broad spectrum of human cancers. However, the therapeutic effect of the recombinant human IL-24 (rhIL-24) protein purified from prokaryotic cells on gastric cancer has not been reported. In this study, we purified soluble rhIL-24 using Q-Sepharose column after the denaturing and renaturing process from the protein of Escherichia coli BL21 transfected with pET-21a(+)-hIL-24 vector and treated by isopropyl-beta-D-1-thiogalactopyranoside (IPTG) for enhanced expression of transgene rhIL-24. We demonstrated that rhIL-24 was capable of inducing in vitro apoptosis of SGC7901 gastric cancer cells and activating peripheral blood mononuclear cellsto secrete cytokines such as IL-6, TNF-alpha, and IFN-gamma. We also showed that rhIL-24 was able to inhibit formation of blood capillaries on chicken embryonic allantois and in vivo tumor angiogenesis leading to suppressing SGC7901 gastric cancer cell growth in vitro and in vivo possibly due to its downregulation of Bcl-2/Bax ratio, VEGF (vascular endothelial growth factor), and CD34. Therefore, our results indicate that rhIL-24 has potent suppressive effect on human SGC7901 gastric carcinoma cell line and warrant its further investigation for therapeutic application against gastric cancer.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Pingyangmycin as a 99mTc carrier in tumor localization

Cited by 3 publications

References 5 publications

Liquid Scintillation Counting Methodology for ⁹⁹Tc Analysis: A Remedy for Radiopharmaceutical Waste

Liquid Scintillation Counting Methodology for ⁹⁹Tc Analysis: A Remedy for Radiopharmaceutical Waste

Recombinant Human IL-24 Suppresses Lung Carcinoma Cell Growth via Induction of Cell Apoptosis and Inhibition of Tumor Angiogenesis

Recombinant Human Interleukin-24 Suppresses Gastric Carcinoma Cell GrowthIn VitroandIn Vivo

Contact Info

Product

Resources

About

Pingyangmycin as a 99mTc carrier in tumor localization

Cited by 3 publications

References 5 publications

Liquid Scintillation Counting Methodology for 99Tc Analysis: A Remedy for Radiopharmaceutical Waste

Liquid Scintillation Counting Methodology for 99Tc Analysis: A Remedy for Radiopharmaceutical Waste

Recombinant Human IL-24 Suppresses Lung Carcinoma Cell Growth via Induction of Cell Apoptosis and Inhibition of Tumor Angiogenesis

Recombinant Human Interleukin-24 Suppresses Gastric Carcinoma Cell GrowthIn VitroandIn Vivo

Contact Info

Product

Resources

About

Liquid Scintillation Counting Methodology for ⁹⁹Tc Analysis: A Remedy for Radiopharmaceutical Waste

Liquid Scintillation Counting Methodology for ⁹⁹Tc Analysis: A Remedy for Radiopharmaceutical Waste