Pinocembrin enriched fraction of Elytranthe parasitica (L.) Danser induces apoptosis in HCT 116 colorectal cancer cells

Kumar, Nishikant; Biswas, Subhankar; Shrungeswara, Akhila Hosur; Mallik, Sanchari Basu; Viji, Mathew Hipolith; Mathew, Jessy Elizabeth; Mathew, Jesil A; Nandakumar, Krishnadas; Lobo, Richard

doi:10.1016/j.jiac.2017.02.009

Cited by 14 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some papers that have investigated the effect on the cell cycle of some of the single compounds identified with HPLC. As regards flavonoids (which are almost completely absent in the digested fraction), pinocembrin was found to block the cell cycle of human prostatic cancer cells (LNCaP) in the S phase [53] and the same effect was observed in hepatocellular carcinoma (HepG2) [54]. Another As regards the regulation of the cell cycle by DMH, a statistically significant increase (p < 0.05) can be noted in the number of cells present in the Sub-G1 phase, including both apoptotic and oncotic cells: the percentage of cells in this phase was, respectively, 26% and 44% more than control with treatment at 16 and 24 mg/mL.…”

Section: Mh and Dmh Induce Cell Cycle Arrestmentioning

confidence: 91%

“…Some papers that have investigated the effect on the cell cycle of some of the single compounds identified with HPLC. As regards flavonoids (which are almost completely absent in the digested fraction), pinocembrin was found to block the cell cycle of human prostatic cancer cells (LNCaP) in the S phase [53] and the same effect was observed in hepatocellular carcinoma (HepG2) [54]. Another flavonoid found only in honey not subjected to digestion, quercetin, has shown a similar effect, blocking the CSCs derived from colorectal carcinoma (HT-29) in the S phase [55], and also in three leukemic cell lines (CEM, K562, Nalm6) and in two breast cancer cell lines (T47D and EAC) [56].…”

Section: Mh and Dmh Induce Cell Cycle Arrestmentioning

confidence: 91%

See 1 more Smart Citation

The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey

et al. 2020

View full text Add to dashboard Cite

Manuka honey (MH) is a natural food with many beneficial properties to human health, thanks to its high variety of bioactive compounds; however, little is known about its bioaccessibility. The aim of this study was to evaluate and compare the polyphenol compounds, the antioxidant capacity and the anticancer activity of MH subjected to an in vitro gastrointestinal digestion in human HCT-116 colon cancer cells. Raw MH and digested MH (DMH) were assessed for total polyphenols and flavonoids by spectrophotometric and HPLC-ESI-MS/MS analysis, and total antioxidant capacity (TAC) using different methods. Cell viability, intracellular ROS production, apoptosis, cell cycle and colony formation capacity were tested after treatment with MH or DMH. Results showed that total polyphenols, total flavonoids and TAC were significantly (p < 0.05) reduced after in vitro digestion. In addition, MH and DMH at 8, 16 and 24 mg/mL had similar effects in inducing intracellular ROS production and in inhibiting the colon formation ability; MH induced a more marked apoptosis compared to DMH, while cell cycle was blocked in S phase by MH and in Sub G1 phase by DMH. Our results increase knowledge of the effect of gastrointestinal digestion on the biological effect of honey against colorectal cancer.

show abstract

Section: Mh and Dmh Induce Cell Cycle Arrestmentioning

confidence: 91%

“…Some papers that have investigated the effect on the cell cycle of some of the single compounds identified with HPLC. As regards flavonoids (which are almost completely absent in the digested fraction), pinocembrin was found to block the cell cycle of human prostatic cancer cells (LNCaP) in the S phase [53] and the same effect was observed in hepatocellular carcinoma (HepG2) [54]. Another flavonoid found only in honey not subjected to digestion, quercetin, has shown a similar effect, blocking the CSCs derived from colorectal carcinoma (HT-29) in the S phase [55], and also in three leukemic cell lines (CEM, K562, Nalm6) and in two breast cancer cell lines (T47D and EAC) [56].…”

Section: Mh and Dmh Induce Cell Cycle Arrestmentioning

confidence: 91%

The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey

et al. 2020

View full text Add to dashboard Cite

show abstract

“…the treatment of microbial infection (7), inflammation (8), ischemia-reperfusion injury (9), and atherosclerosis. In addition, recent studies have demonstrated the antitumor activities of PCB (11)(12)(13)(14). Therefore, the aim of the present study was to investigate the effects of PCB on BC and to identify potential underlying molecular mechanisms.…”

Section: Pcb Treatment Inhibited Activity Of the Pi3k/akt Signaling Pathway In Bc Cellsmentioning

confidence: 99%

“…The induction of apoptosis of cancer cells is a common mechanism of antineoplastic agents (28). Previous studies have demonstrated that PCB inhibits the proliferation of colorectal cancer and ovarian cancer cells via the induction of apoptosis (11,13,14).…”

Section: Pcb Treatment Inhibited Activity Of the Pi3k/akt Signaling Pathway In Bc Cellsmentioning

confidence: 99%

“…Pinocembrin (PCB; 5,7-dihydroxyflavanone) is a pharmacologically active ingredient of Pinus heartwood, Populus, Sparattosperma leucanthum, Eucalyptus, and Euphorbia with diverse pharmacological effects (6) that has been extensively applied for the treatment of microbial infection (7), inflammation (8), ischemia-reperfusion injury (9), and atherosclerosis (10). In addition, numerous recent studies have reported that PCB has anticancer activities by targeting the cell cycle, apoptosis, and the metastatic potential of various solid tumors (11)(12)(13)(14). However, the effect of PCB for the treatment of BC remains unclear.…”

Section: Highlights Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pinocembrin Inhibits the Proliferation and Metastasis of Breast Cancer via Suppression of the PI3K/AKT Signaling Pathway

Zhu¹,

Li²,

Wang³

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

The survival rate of breast cancer (BC) patients remains poor, thus the identification of safe and effective new drugs is crucial to improve therapeutic outcomes and overall survival. Pinocembrin (PCB), a pharmacologically active ingredient of Pinus heartwood, Eucalyptus, Euphorbia, Populus, and Sparattosperma leucanthum, has been widely applied for the treatment of various diseases and possesses anticancer activities. In vitro assays were performed to investigate the antiproliferation and antimetastasis activities of PCB in BC cells. A tumorigenesis assay with the use of murine BC models was performed to assess the antiproliferation activities of PCB in vivo. Moreover, the molecular mechanisms underlying the anticancer activities of PCB in BC cells were explored. The results showed that the anti-inhibitory and antiproliferation activities of PCB in BC might involve cell cycle (G2/M phase) arrest and apoptosis. PCB downregulated the expression levels of proteins involved in cell cycle progression and apoptosis, including cyclinB1, Cdc2, PARP1, Bcl-2, and survivin, and upregulated protein levels of cleaved PARP1, cleaved caspase3, cleaved caspase9, and BAX. In a murine subcutaneous tumor model, PCB suppressed the growth of MCF-7 cells in vivo. Low concentrations of PCB also significantly inhibited the migration and invasion abilities of BC cells. Mechanistically, PCB administration was correlated to suppression of the PI3K/AKT signaling pathway. Inhibition of the proliferation of BC cells by PCB involved cell cycle (G2/M phase) arrest and apoptosis in vitro and in vivo. Low concentrations of PCB also significantly inhibited the migration and invasion abilities of BC cells. These findings suggest that PCB might be an effective agent for treatment of BC patients.

show abstract

Pinocembrin Relieves Mycoplasma pneumoniae Infection‑Induced Pneumonia in Mice Through the Inhibition of Oxidative Stress and Inflammatory Response

Qian

Xue

2022

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Pinocembrin enriched fraction of Elytranthe parasitica (L.) Danser induces apoptosis in HCT 116 colorectal cancer cells

Cited by 14 publications

References 26 publications

The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey

The Influence of In Vitro Gastrointestinal Digestion on the Anticancer Activity of Manuka Honey

Pinocembrin Inhibits the Proliferation and Metastasis of Breast Cancer via Suppression of the PI3K/AKT Signaling Pathway

Pinocembrin Relieves Mycoplasma pneumoniae Infection‑Induced Pneumonia in Mice Through the Inhibition of Oxidative Stress and Inflammatory Response

Contact Info

Product

Resources

About