Pinolenic acid inhibits human breast cancer MDA-MB-231 cell metastasis in vitro

Chen, Szu-Jung; Hsu, Chih‐Ping; Li, Chi-Wei; Lu, Jui-Hua; Chuang, Lu-Te

doi:10.1016/j.foodchem.2010.12.064

Cited by 36 publications

(26 citation statements)

References 26 publications

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“…In cell culture studies, SCA replaced AA in phospholipids [8,9] and inhibited prostaglandin E 2 (PGE 2 ) synthesis and type-2 cyclooxygenase (COX-2) expression in human keratinocytes and murine macrophages [5,10,11]. Our recent studies also demonstrated that PNA and Δ7-ETrA substituted for AA in phospholipids and reduced the production of pro-inflammatory mediators in RAW264.7 macrophages and human breast cancer MDA-MB-231 cells [12][13][14]. Since incorporation of NMIFA into cellular phospholipids is highly correlated with suppression of production of pro-inflammatory mediators in macrophages, we hypothesized that incorporation of NMIFA into microglia, which are the resident macrophages of the brain and spinal cord, would also modulate immune responses in the central nerve system (CNS).…”

Section: Introductionmentioning

confidence: 89%

Phospholipid Incorporation of Non-Methylene-Interrupted Fatty Acids (NMIFA) in Murine Microglial BV-2 Cells Reduces Pro-Inflammatory Mediator Production

et al. 2015

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Sciadonic acid (SCA), pinolenic acid (PNA), and Δ7-eicosatrienoic acid (Δ7-ETrA) are three non-methylene-interrupted fatty acids (NMIFA). Using murine microglial BV-2 cells, this study determined how NMIFA incorporation modulated phospholipid fatty acid composition and the production of pro-inflammatory mediators. Each NMIFA was rapidly taken up and incorporated in BV-2 cells, resulting in the differential redistribution of total lipids. The cellular phospholipid fatty acid compositions were altered, and a significant decrease in the proportions of total monounsaturated fatty acids (MUFA) was observed while the proportions of NMIFA and its metabolites accounted for 38% of the fatty acid total. Incubation of microglial cells with NMIFA suppressed production of LPS-stimulated pro-inflammatory mediators, including nitric oxide (NO), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), as well as the over-expression of inducible nitric oxide synthase (iNOS) and type 2 cyclooxygenase (COX-2). These inhibitory effects could be accounted for, in part, by the inactivation of mitogen-activated protein kinases (MAPK) signaling. In conclusion, Δ7-ETrA, PNA, and SCA are anti-inflammatory NMIFA that may be useful in suppressing in vitro immune responses involved in neural inflammation.

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Section: Introductionmentioning

confidence: 89%

Phospholipid Incorporation of Non-Methylene-Interrupted Fatty Acids (NMIFA) in Murine Microglial BV-2 Cells Reduces Pro-Inflammatory Mediator Production

et al. 2015

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“…Identification of the protein responsible for PA/CA synthesis offers the opportunity to produce such FAs in transgenic plants for large scale productions. Indeed, PA has been demonstrated to confer a number of beneficial effects for example, it has been shown to lower TAG intake in rat (Asset et al, 1999) and has also been shown to inhibit human breast cancer cell metastasis in vitro (Chen et al, 2011).…”

Section: Crfad7 Crfad7mentioning

confidence: 99%

Metabolism of acyl‐lipids in Chlamydomonas reinhardtii

2015

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SUMMARYMicroalgae are emerging platforms for production of a suite of compounds targeting several markets, including food, nutraceuticals, green chemicals, and biofuels. Many of these products, such as biodiesel or polyunsaturated fatty acids (PUFAs), derive from lipid metabolism. A general picture of lipid metabolism in microalgae has been deduced from well characterized pathways of fungi and land plants, but recent advances in molecular and genetic analyses of microalgae have uncovered unique features, pointing out the necessity to study lipid metabolism in microalgae themselves. In the past 10 years, in addition to its traditional role as a model for photosynthetic and flagellar motility processes, Chlamydomonas reinhardtii has emerged as a model organism to study lipid metabolism in green microalgae. Here, after summarizing data on total fatty acid composition, distribution of acyl-lipid classes, and major acyl-lipid molecular species found in C. reinhardtii, we review the current knowledge on the known or putative steps for fatty acid synthesis, glycerolipid desaturation and assembly, membrane lipid turnover, and oil remobilization. A list of characterized or putative enzymes for the major steps of acyl-lipid metabolism in C. reinhardtii is included, and subcellular localizations and phenotypes of associated mutants are discussed. Biogenesis and composition of Chlamydomonas lipid droplets and the potential importance of lipolytic processes in increasing cellular oil content are also highlighted.

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“…Pine nut oil is extracted from pine nut ( Pinus pinea ) and has been widely used for inflammation, appetite, body weight, blood lipids, insulin sensitivity, cancer metastasis, liver steatosis and hyperinsulinemia . Pine nut oil can be extracted by cold pressing, solvent extraction and supercritical CO 2 extraction.…”

Section: Introductionmentioning

confidence: 99%

“…3 Pine nut oil is extracted from pine nut (Pinus pinea) and has been widely used for inflammation, appetite, body weight, blood lipids, insulin sensitivity, cancer metastasis, liver steatosis and hyperinsulinemia. [4][5][6][7][8][9] Pine nut oil can be extracted by cold pressing, solvent extraction and supercritical CO 2 extraction. Several types of fatty acids exist in pine nut oil, comprising 50% polyunsaturated fatty acids, 40% monounsaturated fatty acids and 10% saturated fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Preparation of microcapsule antioxidative wall materials of pine nut oil by the Maillard reaction

2019

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BACKGROUND Maillard reaction products contribute to the amelioration of the biological functions or physical properties of foods and can be used to make dependable antioxidant wall materials for microcapsules of pine nut oil. The present study aimed to analyze the effects of temperature on the Maillard reaction of dry heat processes using gelatin/gum arabic (GE/GA) or gelatin/gum arabic/maltodextrin (GE/GA/MD) models and the products of the Maillard reaction as encapsulants to protect pine nut oil, as well as to evaluate the characteristics of the microcapsules. RESULTS The grafting degree of the product increased with the temperature increments during the Maillard reaction. Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis showed that the polysaccharide covalently linked to the protein. The antioxidant capability of the Maillard products at 80 °C was the highest. The 2,2‐diphenyl‐1‐picrylhydrazyl radical‐scavenging activity, lipid peroxidation‐inhibiting activity and reducing power of the GE/GA/MD model were higher than those of the GE/GA model. With in vitro digestion of Maillard products, GE/GA/MD pine nut oil microcapsules exhibited greater oil release in artificial gastric and enteric juices. Microencapsulated pine nut oil had more stable oxygen, which protected the oil, compared to unencapsulated pine nut oil. CONCLUSION Temperature affects the degree of the Maillard reaction on GE/GA and GE/GA/MD models. © 2018 Society of Chemical Industry

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Pinolenic acid inhibits human breast cancer MDA-MB-231 cell metastasis in vitro

Cited by 36 publications

References 26 publications

Phospholipid Incorporation of Non-Methylene-Interrupted Fatty Acids (NMIFA) in Murine Microglial BV-2 Cells Reduces Pro-Inflammatory Mediator Production

Phospholipid Incorporation of Non-Methylene-Interrupted Fatty Acids (NMIFA) in Murine Microglial BV-2 Cells Reduces Pro-Inflammatory Mediator Production

Metabolism of acyl‐lipids in Chlamydomonas reinhardtii

Preparation of microcapsule antioxidative wall materials of pine nut oil by the Maillard reaction

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