Pinus nigra–Sphaeropsis sapinea as a model pathosystem to investigate local and systemic effects of fungal infection of pines

Bonello, Pierluigi; Blodgett, J. T.

doi:10.1016/j.pmpp.2004.02.002

Cited by 153 publications

(133 citation statements)

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“…Taxifolin, originally identified in pine (Pinus sp.) because of its antifungal activity (Bonello and Blodgett, 2003), was also found in class c.…”

Section: Data Set Explorationmentioning

confidence: 96%

“…9) indicated that withering involves transcriptomic, proteomic, and metabolomic changes representing a stress response to dehydration and eventual pathogen attack. The metabolome data set showed that stilbenes (38, 128, and 230), flavanones (7, 18, 19, 20, 46, 68, 69, 72, 83, and 185), and acylated anthocyanins (12, 13, 22, 35, 36, 58, 60, 81, 107, 112, 115, 127, 215, 237, 247, 280, and 380) are the predominant metabolites that accumulate during withering, with stilbenes and flavonones already implicated in stress responses (Dercks and Creasy, 1989;Adrian et al, 1997;Versari et al, 2001;Bonello and Blodgett, 2003) and a similar role likely for acetylated anthocyanins. The treatment of Merolt grapevine with benzothiadiazole increases the synthesis of trans-resveratrol and anthocyanins, particularly malvidine-3-glucoside and its acylated derivates, and thus enhances resistance to Botrytis cinerea (Iriti et al, 2004).…”

Section: Integration Of Data Sets (Hypothesis-driven Approach)mentioning

confidence: 99%

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Identification of Putative Stage-Specific Grapevine Berry Biomarkers and Omics Data Integration into Networks

Zamboni

Carli²,

Guzzo³

et al. 2010

Plant Physiology

150

135

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The analysis of grapevine (Vitis vinifera) berries at the transcriptomic, proteomic, and metabolomic levels can provide great insight into the molecular events underlying berry development and postharvest drying (withering). However, the large and very different data sets produced by such investigations are difficult to integrate. Here, we report the identification of putative stage-specific biomarkers for berry development and withering and, to our knowledge, the first integrated systems-level study of these processes. Transcriptomic, proteomic, and metabolomic data were integrated using two different strategies, one hypothesis free and the other hypothesis driven. A multistep hypothesis-free approach was applied to data from four developmental stages and three withering intervals, with integration achieved using a hierarchical clustering strategy based on the multivariate bidirectional orthogonal projections to latent structures technique. This identified stage-specific functional networks of linked transcripts, proteins, and metabolites, providing important insights into the key molecular processes that determine the quality characteristics of wine. The hypothesis-driven approach was used to integrate data from three withering intervals, starting with subdata sets of transcripts, proteins, and metabolites. We identified transcripts and proteins that were modulated during withering as well as specific classes of metabolites that accumulated at the same time and used these to select subdata sets of variables. The multivariate bidirectional orthogonal projections to latent structures technique was then used to integrate the subdata sets, identifying variables representing selected molecular processes that take place specifically during berry withering. The impact of this holistic approach on our knowledge of grapevine berry development and withering is discussed.

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“…Taxifolin, originally identified in pine (Pinus sp.) because of its antifungal activity (Bonello and Blodgett, 2003), was also found in class c.…”

Section: Data Set Explorationmentioning

confidence: 96%

Section: Integration Of Data Sets (Hypothesis-driven Approach)mentioning

confidence: 99%

Identification of Putative Stage-Specific Grapevine Berry Biomarkers and Omics Data Integration into Networks

Zamboni

Carli²,

Guzzo³

et al. 2010

Plant Physiology

150

135

View full text Add to dashboard Cite

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“…This mechanism occurs not only in herbaceous plants (Durrant and Dong, 2004), but also in different conifer tree species (Bonello et al, 2001;Bonello and Blodgett, 2003;Luchi et al, 2005;Wallis et al, 2007).…”

Section: Tablementioning

confidence: 99%

Monoterpene responses to interacting effects of drought stress and infection by the fungus Heterobasidion parviporum in two clones of Norway spruce (Picea abies)

Madmony

Tognetti

Zamponi

et al. 2018

Environmental and Experimental Botany

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A B S T R A C TTo study simultaneous biotic and abiotic stress effects on monoterpene mediated defence response, four-year-old seedlings of two clones of Picea abies (c386: "relatively resistant" c386; c171: "relatively susceptible" clone 171) were inoculated with an isolate of rot fungus Heterobasidion parviporum and simultaneously subjected to a drought stress recovery cycle. Fungal growth into the wood was generally higher in well-watered (W) than in drought-stressed (D) plants. As predawn water potential decreased, gas exchange and maximal photochemical efficiency of PSII also declined. Water potential and chlorophyll fluorescence showed differences between clones in D plants, c386 being more tolerant than c171, while stomatal conductance and net photosynthesis differed between clones in W plants; c171 showing higher values than c386. A recovery of physiological functions was observed after re-watering. The clones showed different constitutive monoterpene profiles. The infection generally caused some extensive compositional changes in the relative contents of several monoterpenes and their enantiomers; also, sterile infection (wounded only samples) affected the relative proportions of monoterpenes, although to a lesser extent than reactions elicited by the fungus. The trend in monoterpene response to disease treatments was the same in D and W plants of both clones. In particular, higher proportion of δ-3-carene was found constitutively in c386 in comparison with c171; besides, infection with H. parviporum increased the relative contents of δ-3-carene in all the samples, even if proportions of this monoterpene in wounded plus inoculated tissues of c386 were significantly higher than cortical tissues from non-inoculated branches only in W seedlings 44 days after treatment.Although drought stress decreased the total absolute contents of monoterpenes, total monoterpene concentrations significantly increased in response to infection by H. parviporum.

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“…While many studies have demonstrated that induction of phenolics occurs at a local scale (e.g., Krokene et al 1999;Evensen et al 2000;Franceschi et al 2000), several recent studies have also found evidence for systemic induction of soluble low molecular weight phenolics and lignin (e.g., Bonello and Blodgett 2003;Bonello et al 2006;Wallis et al 2008). The activation of phenolic metabolism and the accumulation of certain phenolics appear to be commonly associated with the phenomenon referred to as systemic induced resistance to pathogens (SIR).…”

Section: Induced Phenolicsmentioning

confidence: 99%

Phenolic metabolites in the resistance of northern forest trees to pathogens — past experiences and future prospects

2008

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Phenolic metabolites are frequently implicated in chemical defense mechanisms against pathogens in woody plants. However, tree breeding programmes for resistance to pathogens and practical tree-protection applications based on these compounds seem to be scarce. To identify gaps in our current knowledge of this subject, we explored some of the recent literature on the involvement of phenolic metabolites in the resistance of northern forest trees (Pinus, Picea, Betula, Populus, and Salix spp.) to pathogens. Although it is evident that the phenolic metabolism of trees is often activated by pathogen attacks, few studies have convincingly established that this induction is due to a specific defense response that is capable of stopping the invading pathogen. The role of constitutive phenolics in the resistance of trees to pathogens has also remained unclear. In future studies, the importance of phenolics in oxidative stress, cell homeostasis and tolerance, and the spatial and temporal localization of phenolics in relation to invading pathogens should be more carefully acknowledged. Possibilities for future studies using advanced methods (e.g., metabolic profiling, confocal laser scanning microscopy, and use of modified tree genotypes) are discussed.Résumé : Les métabolites phénoliques sont souvent impliqués dans les mécanismes de défense chimiques contre les agents pathogènes chez les plantes ligneuses. Cependant, il semble y avoir peu de programmes d'amélioration des arbres pour la résistance aux agents pathogènes ou de mesures pratiques de protection des arbres qui sont basés sur ces composés. Dans le but d'identifier les lacunes dans nos connaissances actuelles sur ce sujet, nous avons exploré une partie de la littérature récente traitant du rôle des métabolites phénoliques dans la résistance des espèces forestières nordiques (Pinus, Picea, Betula, Populus et Salix spp.) aux agents pathogènes. Bien qu'il soit évident que le métabolisme des composés phénoliques chez les arbres est souvent déclenché par les attaques des agents pathogènes, il a rarement été établi de façon convaincante que cette induction est due à une réaction de défense spécifique capable d'enrayer l'invasion de l'agent pathogène. De plus, on n'est toujours pas certain du rôle que jouent les composés phénoliques constitutifs dans la résistance des arbres face aux agents pathogènes. Dans les études ultérieures, on devrait accorder plus d'attention à l'importance des composés phénoliques en lien avec le stress oxydatif, l'homéostasie et la tolérance des cellules ainsi qu'à la localisation spatiale et temporelle des composés phénoliques en relation avec l'invasion des agents pathogènes. La discussion porte sur la possibilité que les études futures utilisent des méthodes de pointes telles que le profilage métabolique, le microscope confocal à balayage laser et aient recours à des arbres dont le génotype a été modifié.[Traduit par la Rédaction]

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Pinus nigra–Sphaeropsis sapinea as a model pathosystem to investigate local and systemic effects of fungal infection of pines

Cited by 153 publications

References 46 publications

Identification of Putative Stage-Specific Grapevine Berry Biomarkers and Omics Data Integration into Networks

Identification of Putative Stage-Specific Grapevine Berry Biomarkers and Omics Data Integration into Networks

Monoterpene responses to interacting effects of drought stress and infection by the fungus Heterobasidion parviporum in two clones of Norway spruce (Picea abies)

Phenolic metabolites in the resistance of northern forest trees to pathogens — past experiences and future prospects

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