31 Mediterranean mountainous environments are biodiversity hotspots and priority areas in 32 conservation agendas. Although they are fragile and threatened by forecasted global 33 change scenarios, their sensitivity to long-term environmental variability is still 34 understudied. The Sierra Nevada range, located in southern Spain on the north-western 35 European flanks of the Mediterranean basin, is a biodiversity hotspot. Consequently, 36 Sierra Nevada provides an excellent model system to apply a palaeoecological approach 37 to detect vegetation changes, explore the drivers triggering those changes, and how 38 vegetation changes link to the present landscape in such a paradigmatic mountain system. 39 A multi-proxy strategy (magnetic susceptibility, grain size, loss-on-ignition, macroremains, 40 charcoal and palynological analyses) is applied to an 8400-year long lacustrine 41 environmental archive from the Laguna de la Mosca (2889 masl). The long-term ecological 42 data show how the Early Holocene pine forests transitioned towards mixed Pinus-Quercus 43 submediterranean forests as a response to a decrease in seasonality at ~7.3 cal. kyr BP. 44 The mixed Pinus-Quercus submediterranean forests collapsed drastically giving way to 45 open evergreen Quercus formations at ~4.2 cal. kyr BP after a well-known aridity crisis. 46 Under the forecasted northward expansion of the Mediterranean area due to global 47 change-related aridity increase, mountain forests inhabiting territories adjacent to the 48 Mediterranean Region could experience analogous responses to those detected in the 49 Sierra Nevada forests to the Mid to Late Holocene aridification, moving from temperate to 50 submediterranean and then Mediterranean formations. 51 52 53