Pioglitazone Lowers Serum Retinol Binding Protein 4 by Suppressing its Expression in Adipose Tissue of Obese Rats

Zhu, Changlian; Xiao, Yuanyuan; Liu, Xiaohua; Han, Junfeng; Zhang, Jianmei; Li, Wei; Wang, Jia

doi:10.1159/000369737

Cited by 14 publications

(7 citation statements)

References 27 publications

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“…Intriguingly, our result showed that the RBP4 mRNA expression level in adipose tissue was considerably increased in rats fed HFD. This result is confirmed by the previous study (Zhu et al, 2015). On the other side, the GLUT4 mRNA expression level in adipose tissue was substantially reduced by HFD.…”

Section: Discussionsupporting

confidence: 91%

Linseed oil supplementation improves altered lipid metabolism and insulin resistance in induced obese rats

Seliem

Azab

Ismaila

et al. 2022

Benha Veterinary Medical Journal

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The present work was performed to elucidate the effect of linseed oil supplementation on metabolic disorders in induced obese male albino rats. Thirty male Albino Wister rats were used in this investigation. They were categorized into two groups; 10 rats received a controldiet, and 20 rats were supplied a high-fat diet for two months till the induction of obesity. Then, rats were split into 3 groups (10 rats each). Group I was maintained on a control diet. Group II was maintained on HFD only. group III received HFD with 30% linseed oil for further two months. At the end of the experiment, the rat's body and organs weights were assessed. Blood samples and adipose tissue were obtained for analysis. The results revealed that linseed oil supplementation caused a substantial (P < 0.05) reduction in serum TC, TAG, LDL-C, VLDL-C, glucose, insulin levels, and HOMA-IR. However, HDL-C and adiponectin levels showed a significant (P < 0.05) increase when compared with the HFD group. Linseed oil supplementation also caused significant down regulation of retinol-binding protein 4 (RBP4) gene expression, however, the GLUT4 gene showed significant upregulation. From the obtained results it could be concluded that linseed oil has an ameliorative effect on altered lipid profile caused by obesity. In addition, linseed oil caused improvement of insulin resistance in addition to the pro and anti-inflammatory biomarkers GLUT4 Insulin resistance Linseed oil Obesity RBP4

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Section: Discussionsupporting

confidence: 91%

Linseed oil supplementation improves altered lipid metabolism and insulin resistance in induced obese rats

Seliem

Azab

Ismaila

et al. 2022

Benha Veterinary Medical Journal

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“…The effect of TZD on the level of retinol in goats in the first experiment is of interest. It has been previously shown that treatment with pioglitazone, a thiazolidinedione molecule, decreased retinol binding protein 4 (RBP) in the serum of rats, as well as decreasing the expression of Rbp4 in 3T3-L1 adipocytes (Zhu et al ., 2015). The reduction of serum retinol by TZD detected in the first experiment can be attributed to the fact that RBP transports retinol in the blood from liver storage to peripheral tissues (Zabetian-Targhi et al ., 2015).…”

Section: Discussionmentioning

confidence: 99%

Effects of 2,4-thiazolidinedione (TZD) on milk fatty acid profile and serum vitamins in dairy goats challenged with intramammary infusion of Streptococcus uberis

Tsai

Rosa

Bionaz

et al. 2020

Journal of Dairy Research

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The study included two experiments. In the first, 24 lactating Saanen dairy goats received low-energy diet without vitamin supplements. Twelve goats received a daily IV injection of 2,4- thiazolidinedione (TZD), others received saline injection. A week later, 6 goats from each treatment were challenged with intramammary infusion (IMI) of saline (CTRL) or Streptococcus uberis. In the second experiment, 12 Saanen lactating dairy goats received supplemental vitamins to reach NRC recommendation level. Six goats in each group were injected with TZD or saline daily, and 14 d later received Streptococcus uberis IMI in the right half of the udder. The hypotheses were (1) TZD does not affect the level of retinol in blood, and (2) the fatty acid profile is affected by the interaction between mammary infection and TZD in dairy goats. In the first experiment blood samples were collected on d −7, −2, 1, 2, 12 and milk samples were collected on d −8, 1, 4, 7, and 12, both relative to IMI. In the second experiment, blood samples were collected on d −15, 0, 1, and 10 relative to IMI. Milk and serum samples were analyzed for retinol, α-tocopherol and fatty acid profile. Serum retinol and β-carotene concentrations were higher in the second experiment compared to the first. Serum β-carotene and α-tocopherol were greater in TZD than CTRL and there was a TZD × time interaction in the first experiment. In addition, the TZD × time interaction showed that the milk fatty acid were reduced in C16 : 0 while C18 : 3 n3 while total omega 3 fatty acids were increased, as well as with minor effect on preventing a transient increase in α-tocopherol in milk. Overall, the TZD may affect the lipid-soluble vitamins and fatty acid profile, potentially altering immune responses, during mastitis in dairy goats.

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“…For example, rosiglitazone decreases RBP4 levels in the adipose tissue of Glut4 −/− mice ( 33 ). In addition, pioglitazone significantly decreases levels of RBP4 in an obese rat model, decreases body weight and improves insulin sensitivity ( 94 ). Finally, sitagliptin, a dipeptidyl peptidase-4 inhibitor, decreases RBP4 expression and improves insulin sensitivity in diabetic rats ( 95 ).…”

Section: Clinical Applications Of Rbp4 and Future Perspectivesmentioning

confidence: 99%

Interplay of retinol binding protein 4 with obesity and associated chronic alterations (Review)

et al. 2022

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Obesity is a multifactorial disease, defined as excessive fat deposition in adipose tissue. Adipose tissue is responsible for the production and secretion of numerous adipokines that induce metabolic disorders. Retinol-binding protein 4 (RBP4) is an adipokine that transports vitamin A or retinol in the blood. High levels of RBP4 are associated with development of metabolic disease, including obesity, insulin resistance (IR), metabolic syndrome, and type 2 diabetes (T2D). The present review summarizes the role of RBP4 in obesity and associated chronic alterations. Excessive synthesis of RBP4 contributes to inflammatory characteristic of obesity by activation of immune cells and release of proinflammatory cytokines, such as TNFα and ILs, via the Toll-like receptor/JNK pathway. The retinol-RBP4 complex inhibits insulin signaling directly in adipocytes by activating Janus kinase 2 (JAK2)/STAT5/suppressor of cytokine signaling 3 signaling. This mechanism is retinol-dependent and requires vitamin A receptor stimulation by retinoic acid 6 (STRA6). In muscle, RBP4 is associated with increased serine 307 phosphorylation of insulin receptor substrate-1, which decreases its affinity to PI3K and promotes IR. In the liver, RBP4 increases hepatic expression of phosphoenolpyruvate carboxykinase, which increases production of glucose. Elevated serum RBP4 levels are associated with β-cell dysfunction in T2D via the STRA6/JAK2/STAT1/insulin gene enhancer protein 1 pathway. By contrast, RBP4 induces endothelial inflammation via the NF-κB/nicotinamide adenine dinucleotide phosphate oxidase pathway independently of retinol and STRA6, which stimulates expression of proinflammatory molecules, such as vascular cell adhesion molecule 1, E-selectin, intercellular adhesion molecule 1, monocyte chemoattractant protein 1 and TNFα. RBP4 promotes oxidative stress by decreasing endothelial mitochondrial function; overall, it may serve as a useful biomarker in the diagnosis of obesity and prognosis of associated disease, as well as a potential therapeutic target for treatment of these diseases.

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Pioglitazone Lowers Serum Retinol Binding Protein 4 by Suppressing its Expression in Adipose Tissue of Obese Rats

Cited by 14 publications

References 27 publications

Linseed oil supplementation improves altered lipid metabolism and insulin resistance in induced obese rats

Linseed oil supplementation improves altered lipid metabolism and insulin resistance in induced obese rats

Effects of 2,4-thiazolidinedione (TZD) on milk fatty acid profile and serum vitamins in dairy goats challenged with intramammary infusion of Streptococcus uberis

Interplay of retinol binding protein 4 with obesity and associated chronic alterations (Review)

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