Pioneer marine biofilms on artificial surfaces including antifouling coatings immersed in two contrasting French Mediterranean coast sites

Briand, Jean‐François; Djeridi, Ikram; Jamet, Dominique; Coupé, Stéphane; Bressy, Christine; Molmeret, Maëlle; Berre, Brigitte Le; Rimet, Frédéric; Bouchez, Agnès; Blache, Yves

doi:10.1080/08927014.2012.688957

Cited by 127 publications

(83 citation statements)

References 48 publications

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“…Our results indicated that the amount of bacteria on the surfaces from the control system was 10 8 cells·cm −2 . Similar numbers of bacterial microfouling have been reported on anti-fouling surfaces in oceanic waters, also in much warmer sites [24][25][26]. Hypochlorite treatment reduced the bacterial numbers by 10-1000-fold, but the efficiency depended on the coating.…”

Section: Discussionsupporting

confidence: 51%

Biofouling on Coated Carbon Steel in Cooling Water Cycles Using Brackish Seawater

Rajala

Sohlberg

Priha

et al. 2016

JMSE

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Abstract:Water cooling utilizing natural waters is typically used for cooling large industrial facilities such as power plants. The cooling water cycles are susceptible to biofouling and scaling, which may reduce heat transfer capacity and enhance corrosion. The performance of two fouling-release coatings combined with hypochlorite treatment were studied in a power plant utilizing brackish sea water from the Baltic Sea for cooling. The effect of hypochlorite as an antifouling biocide on material performance and species composition of microfouling formed on coated surfaces was studied during the summer and autumn. Microfouling on surfaces of the studied fouling-release coatings was intensive in the cooling water cycle during the warm summer months. As in most cases in a natural water environment the fouling consisted of both inorganic fouling and biofouling. Chlorination decreased the bacterial number on the surfaces by 10-1000 fold, but the efficacy depended on the coating. In addition to decreasing the bacterial number, the chlorination also changed the microbial species composition, forming the biofilm on the surfaces of two fouling-release coatings. TeknoTar coating was proven to be more efficient in combination with the hypochlorite treatment against microfouling under these experimental conditions.

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Section: Discussionsupporting

confidence: 51%

Biofouling on Coated Carbon Steel in Cooling Water Cycles Using Brackish Seawater

Rajala

Sohlberg

Priha

et al. 2016

JMSE

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“…This technique has been used for counting bacteria in different environmental samples including soils, brackish waters, pond and lake waters, seawater, marine intertidal sediments, natural biofilms and even oyster tissue homogenates (Table A1). Previous studies on marine biofilms developing on surfaces submerged in the sea reported the use of scalpels to remove the biofilm from the substratum prior to enumeration on membrane filters [8][9][10][11][12]. Few studies reported the use of microscope slides to develop a biofilm and consequently enumerate bacteria in their native, hydrated conditions [6,13,14].…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Reliability of Counting Bacteria Using Epifluorescence Microscopy

Muthukrishnan

Govender

Dobretsov

et al. 2017

JMSE

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Abstract:The common practice of counting bacteria using epifluorescence microscopy involves selecting 5-30 random fields of view on a glass slide to calculate the arithmetic mean which is then used to estimate the total bacterial abundance. However, not much is known about the accuracy of the arithmetic mean when it is calculated by selecting random fields of view and its effect on the overall abundance. The aim of this study is to evaluate the accuracy and reliability of the arithmetic mean by estimating total bacterial abundance and to calculate its variance using a bootstrapping technique. Three fixed suspensions obtained from a three-week-old marine biofilm were stained and dispersed on glass slides. Bacterial cells were counted from a total of 13,924 fields of view on each slide. Total bacterial count data obtained were used for calculating the arithmetic mean and associated variance and bias for sample field sizes of 5, 10, 15, 20, 25, 30, 35 and 40. The study revealed a non-uniform distribution of bacterial cells on the glass slide. A minimum of 20 random fields of view or a minimum of 350 bacterial cells need to be counted to obtain a reliable value of the arithmetic mean to estimate the total bacterial abundance for a marine biofilm sample dispersed on a glass slide.

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“…Biofilms can lead to corrosion of metals by increasing the open circuit potential toward positive values by metal deposition at the cathode, oxygen removal and acid production (Videla & Herrera 2005;Landousli et al 2011). While biocidal and foulingrelease coatings are generally free from macrofouling, they are still subject to colonisation by biofilms (Cassé & Swain 2006;Molino et al 2009;Dobretsov & Thomason 2011;Zargiel et al 2011;Briand et al 2012). Biofilms on antifouling (AF) coatings increase roughness and hence drag, which results in increased fuel consumption of vessels (Yebra et al 2006;Schultz et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Mini-review: Inhibition of biofouling by marine microorganisms

2013

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Any natural or artificial substratum exposed to seawater is quickly fouled by marine microorganisms and later by macrofouling species. Microfouling organisms on the surface of a substratum form heterogenic biofilms, which are composed of multiple species of heterotrophic bacteria, cyanobacteria, diatoms, protozoa and fungi. Biofilms on artificial structures create serious problems for industries worldwide, with effects including an increase in drag force and metal corrosion as well as a reduction in heat transfer efficiency. Additionally, microorganisms produce chemical compounds that may induce or inhibit settlement and growth of other fouling organisms. Since the last review by the first author on inhibition of biofouling by marine microbes in 2006, significant progress has been made in the field. Several antimicrobial, antialgal and antilarval compounds have been isolated from heterotrophic marine bacteria, cyanobacteria and fungi. Some of these compounds have multiple bioactivities. Microorganisms are able to disrupt biofilms by inhibition of bacterial signalling and production of enzymes that degrade bacterial signals and polymers. Epibiotic microorganisms associated with marine algae and invertebrates have a high antifouling (AF) potential, which can be used to solve biofouling problems in industry. However, more information about the production of AF compounds by marine microorganisms in situ and their mechanisms of action needs to be obtained. This review focuses on the AF activity of marine heterotrophic bacteria, cyanobacteria and fungi and covers publications from 2006 up to the end of 2012.

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Pioneer marine biofilms on artificial surfaces including antifouling coatings immersed in two contrasting French Mediterranean coast sites

Cited by 127 publications

References 48 publications

Biofouling on Coated Carbon Steel in Cooling Water Cycles Using Brackish Seawater

Biofouling on Coated Carbon Steel in Cooling Water Cycles Using Brackish Seawater

Evaluating the Reliability of Counting Bacteria Using Epifluorescence Microscopy

Mini-review: Inhibition of biofouling by marine microorganisms

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