Pioneers of Television — Charles Francis Jenkins

Abramson, Albert

doi:10.5594/j07850

Cited by 8 publications

(1 citation statement)

References 10 publications

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“…al-Acid glycoprotein (al-AG) has been shown to be one of the proteins binding various basic drugs in human serum (Piafsky & Borga 1977;Piafsky et al 1978;Schley et al 1980;Abramson et al 1982). The concentration of al-AG has wide inter-and intraindividual variability and increases in various disease states and after surgery.…”

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confidence: 99%

Assessment of the contribution of α1-acid glycoprotein to the serum binding of basic drugs using serum treated with sulphosalicylic acid and DEAE-cellulose

Suzuki

Sugiyama

Sawada

et al. 1985

Journal of Pharmacy and Pharmacology

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Treatment of human serum with DEAE-cellulose in acid conditions almost completely removed alpha 1-acid glycoprotein (alpha 1-AG) with little change in the concentration of albumin and beta-lipoprotein, while treatment with sulphosalicylic acid removed almost all the proteins except alpha 1-AG. The binding of various drugs to serum treated as above was measured by equilibrium dialysis and the contribution of alpha 1-AG to drug binding by human serum was assessed. Sulphosalicylic acid-treated serum exhibited a saturable binding for propranolol, which was considered to be due to the binding to alpha 1-AG while DEAE-cellulose-treated serum mostly exhibited non-saturable binding, for which albumin and beta-lipoprotein may be responsible. With this treated serum, alpha 1-AG was estimated to contribute approximately 40% to the binding of therapeutic concentrations of propranolol, 15% to that of imipramine and 15-20% to that of desipramine, respectively, in serum samples pooled from healthy adults. However, no contribution of alpha 1-AG was observed in the binding of salicylic acid to the serum. Dissociation constants of the propranolol binding to the high affinity site in control serum, sulphosalicylic acid-treated serum and purified alpha 1-AG showed similar values (3.7-6.7 microM). These results suggest that treatment of serum with sulphosalicylic acid and DEAE cellulose is useful in assessing the contribution of alpha 1-AG to the serum binding of basic drugs.

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confidence: 99%