PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit

Ovrutska, I.I.; Kordyum, Е.L.

doi:10.15407/dopovidi2019.05.097

Cited by 3 publications

(4 citation statements)

References 14 publications

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Section: Altered Abundance Of Other Critical Stress Sensitive Protein...supporting

confidence: 80%

“…Aquaporins belonging to the class of membrane proteins facilitate water transport and transport of other solutes thereby playing a vital role in cell signaling, nutrient acquisition and stress response [69]. In GM6, aquaporin PIP2-1 was found in higher abundance to ensure the water availability to the plant under stressful conditions, coherent with the previous findings on maize genotypes under drought stress [69]. On the other side, leucine zipper/EFhandcontaining transmembrane protein 1 (LETM1) like protein has a remarkable role in mitochondrial translation in early seed development as studied in Arabidopsis thaliana [70].…”

Section: Altered Abundance Of Other Critical Stress Sensitive Protein...mentioning

confidence: 99%

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Comparative protein analysis of two maize genotypes with contrasting tolerance to low temperature

2023

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Background Low temperature (LT) stress is one of the major environmental stress factors affecting the growth and yield of maize (Zea mays L.). Hence, it is important to unravel the molecular mechanisms behind LT stress tolerance to improve molecular breeding in LT tolerant genotypes. In the present study, two maize genotypes viz. Gurez local from Kashmir Himalaya and tropical grown GM6, were dissected for their LT stress response in terms of accumulation of differentially regulated proteins (DRPs). Leaf proteome analysis at three-leaf stage of maize seedlings subjected to LT stress of 6 °C for a total of 12 h duration was performed using two dimensional gel electrophoresis (2D-PAGE) followed by subsequent identification of the proteins involved. Results After MALDI-TOF (Matrix-assisted laser desorption/ionization-time of flight) and bioinformatics analysis, 19 proteins were successfully identified in Gurez local, while as 10 proteins were found to get successful identification in GM6. The interesting observations from the present investigation is the identification of three novel proteins viz. threonine dehydratase biosynthetic chloroplastic, thylakoidal processing peptidase 1 chloroplastic, and nodulin-like protein, whose role in abiotic stress tolerance, in general, and LT stress, in particular, has not been reported so far. It is important to highlight here that most of LT responsive proteins including the three novel proteins were identified from Gurez local only, owing to its exceptional LT tolerance. From the protein profiles, obtained in both genotypes immediately after LT stress perception, it was inferred that stress responsive protein accumulation and their expression fashion help the Gurez local in seedling establishment and withstand unfavorable conditions as compared to GM6. This was inferred from the findings of pathway enrichment analysis like regulation of seed growth, timing of floral transition, lipid glycosylation, and aspartate family amino acid catabolic processes, besides other key stress defense mechanisms. However, in GM6, metabolic pathways enriched were found to be involved in more general processes including cell cycle DNA replication and regulation of phenylpropanoid metabolism. Furthermore, majority of the qRT-PCR results of the selected proteins demonstrated positive correlation between protein levels and transcript abundance, thereby strengthening our findings. Conclusions In conclusion, our findings reported majority of the identified proteins in Gurez local exhibiting up-regulated pattern under LT stress as compared to GM6. Furthermore, three novel proteins induced by LT stress were found in Gurez local, requiring further functional validation. Therefore, our results offer more insights for elucidating the molecular networks mediating LT stress tolerance in maize.

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Section: Altered Abundance Of Other Critical Stress Sensitive Protein...supporting

confidence: 80%

Section: Altered Abundance Of Other Critical Stress Sensitive Protein...mentioning

confidence: 99%

Comparative protein analysis of two maize genotypes with contrasting tolerance to low temperature

2023

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“…У гібриду з помірною посухостійкістю 'Переяславський' експресія PIP 2; 1 навпаки, дещо знижувалася, а у гібриду 'Яхта' майже не змінювалася. Це свідчить про те, що посилення експресії РІР 2; 1 може бути однією із визначальних ознак посухостійких гібридів (Ovrutska, Kordyum, 2019). Значну відмінність в експресії генів аквапоринів було виявлено у повітряноводних Sium sizaroideum L. та суходільних рослин Sium latifolium L. Експресія РІР 2; 1 суходільної форми посилювалася порівняно з повітряно-водними рослинами і не змінювалась протягом онтогенезу (Bliuma, 2010).…”

Section: Basidiomycota Agaricomycetesunclassified

“…Така різна реакція генів аквапоринів родини РІР 2 у відповідь на водний стрес припускає, що різні ізоформи аквапоринів рослин відіграють різну роль у регуляції водного транспорту (Hachez et al, 2006), що забезпечує широкі адаптаційні можливості рослин. Таким чином, підсумовуючи існуючі дані, можна зробити висновок про те, що посилена експресія PIP 2; 1 є характерною ознакою посухостійких гібридів кукурудзи 'Достаток' та 'Флагман' і, не виключено, однією з визначальних ознак посухостійкості (Ovrutska, Kordyum, 2019).…”

Section: Basidiomycota Agaricomycetesunclassified

Untitled

2021

Ukr. Bot. J.

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https://ukrbotj.co.ua UKRAINIAN BOTANICAL JOURNAL is а scientific journal publishing articles and contributions on all aspects of botany and mycology, including general issues, taxonomy, floristics, vegetation science, ecology, evolutionary biology, geography, history of flora and vegetation as well as morphology, anatomy, physiology, biochemistry, cell and molecular biology of plants and fungi. Original articles, short communications and other contributions are published in sections General Issues and Reviews,

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Aquaporins in regulation of plant protective responses to drought

Ovrutska¹

2021

Ukr. Bot. J.

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Plasmolemma permeability is an integral indicator of the functional state of plant cells under stress. Aquaporins (AQPs), specialized transmembrane proteins that form water channels and play an important role in the adaptation of plants to adverse conditions and, in particular, to lack or excess of water, are involved in the formation of the response to drought. The main function of AQPs is to facilitate the movement of water across cell membranes and maintain aqueous cell homeostasis. Under stressful conditions, there is both an increase and decrease in the expression of individual aquaporin genes. Analysis of the data revealed differences in the expression of AQPs genes in stable and sensitive plant genotypes. It turned out that aquaporins in different stress-resistant varieties of the same species also respond differently to drought. The review provides brief information on the history of the discovery of aquaporins, the structure and function of these proteins, summarizes the latest information on the role of aquaporins in the regulation of metabolism and the response of plants to stressors, with particular emphasis on aquaporins in drought protection. The discovery and study of AQPs expands the possibilities of using genetic engineering methods for the selection of new plant species, in particular, more resistant to drought and salinization of the soil, as well as to increase their productivity. The use of aquaporins in biotechnology to improve drought resistance of various species has many prospects.

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PIP2;1 aquaporin gene expression in maize hybrids different for drought tolerance to water deficit

Cited by 3 publications

References 14 publications

Comparative protein analysis of two maize genotypes with contrasting tolerance to low temperature

Comparative protein analysis of two maize genotypes with contrasting tolerance to low temperature

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Aquaporins in regulation of plant protective responses to drought

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