Piperlongumine induces ROS mediated cell death and synergizes paclitaxel in human intestinal cancer cells

Rawat, Laxminarayan; Hegde, Harsha V.; Hoti, S.L.; Nayak, Vijayashree

doi:10.1016/j.biopha.2020.110243

Cited by 66 publications

(44 citation statements)

References 45 publications

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“…Piperlongumine is a widely studied natural product found in the plant species Piper longum . Intervention with 5 to 15 μM piperlongumine has been previously reported to induce apoptosis through a ROS mediated mechanism (Keshav et al 2017; Wang et al 2019; Rawat et al 2020; Zhou et al 2020). We found that when cells were incubated with 10 μM piperlongumine (BioVision), the downregulation of Cyt-C and caspase 3 was reversed (Fig.…”

Section: Resultsmentioning

confidence: 99%

Cigarette Smoke Exposure Inhibits Osteoclast Apoptosis via the mtROS Pathway

et al. 2021

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It is widely known that smoking is a risk factor for bone loss and plays a key role in osteopenia. Despite this well-known association, the mechanisms by which smoking affects bone have not been definitively established. Since smoking increases bone loss and potentially affects bone resorption in response to mechanical force, we investigated the impact of cigarette smoke on osteoclast numbers and underlying mechanisms in a mouse model of orthodontic tooth movement (OTM). The experimental group was exposed to once-daily cigarette smoke while the control group was not, and tooth movement distance and osteoclast numbers were assessed. In addition, the effect of cigarette smoke extract (CSE) on osteoclast precursor proliferation and osteoclast apoptosis was assessed in vitro. We found that cigarette smoke exposure enhanced bone remodeling stimulated by mechanical force and increased osteoclast numbers in vivo. Also, CSE increased the number of osteoclasts by inhibiting osteoclast apoptosis via the mitochondrial reactive oxygen species/cytochrome C/caspase 3 pathway in vitro. Moreover, exposure of mice to cigarette smoke affected bone marrow cells, leading to increased formation of osteoclasts in vitro. This study identifies a previously unknown mechanism of how smoking has a detrimental impact on bone.

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Section: Resultsmentioning

confidence: 99%

Cigarette Smoke Exposure Inhibits Osteoclast Apoptosis via the mtROS Pathway

et al. 2021

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“…Used in the following research were: 2′-(4-hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazoletrihydrochloride (Hoechst 33258) hydrate, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), and deuterium. 77 KB cell line was purchased from National Centre for Cell Science (NCCS), Pune, India. Chemicals used in the experiments were of molecular and analytical grade.…”

Section: Methodsmentioning

confidence: 99%

“…Absorbance was measured at 570nm in Multiskan™ GO Microplate reader (Thermo scientific, USA); all the cytotoxicity methodology and procedure were done. 77 …”

Section: Methodsmentioning

confidence: 99%

One Pot Synthesis of PEGylated Bimetallic Gold–Silver Nanoparticles for Imaging and Radiosensitization of Oral Cancers

Ahmed¹,

Baijal

Somashekar³

et al. 2021

IJN

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Background:Radiotherapy is an important treatment modality for many types of head and neck squamous cell carcinomas. Nanomaterials comprised of high atomic number (Z) elements are novel radiosensitizers enhance radiation injury by production of free radicals and subsequent DNA damage. Gold nanoparticles are upcoming as promising radiosensitizers due to their high (Z) biocompatibility, and ease for surface engineering. Bimetallic nanoparticles have shown enhanced anticancer activity compared to monometallic nanoparticles. Materials and Methods: PEG-coated Au-Ag alloy nanoparticles (BNPs) were synthesized using facile one pot synthesis techniques. Size of ~50±5nm measured by dynamic light scattering. Morphology, structural composition and elemental mapping were analyzed by electron microscopy and SAXS (small-angle X-ray scattering). The radiosensitization effects on KB oral cancer cells were evaluated by irradiation with 6MV X-rays on linear accelerator. Nuclear damage was imaged using confocal microscopy staining cells with Hoechst stain. Computed tomography (CT) contrast enhancement of BNPs was compared to that of the clinically used agent, Omnipaque. Results: BNPs were synthesized using PEG 600 as reducing and stabilizing agent. The surface charge of well dispersed colloidal BNPs solution was −5mV. Electron microscopy reveals spherical morphology. HAADF-STEM and elemental mapping studies showed that the constituent metals were Au and Ag intermixed nanoalloy. Hydrodynamic diameter was ~50±5nm due to PEG layer and water molecules absorption. SAXS measurement confirmed BNPs size around 35nm. Raman shift of around 20 cm −1 was observed when BNPs were coated with PEG. 1 H NMR showed extended involvement of − OH in synthesis. BNPs efficiently enter cytoplasm of KB cells and demonstrated potent in vitro radiosensitization with enhancement ratio ~1.5-1.7. Imaging Hoechst-stained nuclei demonstrated apoptosis in a dose-dependent manner. BNPs exhibit better CT contrast enhancement ability compared to Omnipaque. Conclusion: This bimetallic intermix nanoparticles could serve a dual function as radiosensitizer and CT contrast agent against oral cancers, and by extension possibly other cancers as well.

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“…Piperlongumine has been identified to induce reactive oxygen species (ROS), thus exhibiting intense anti-cancer activity and sensitizing cells to anti-cancer agents such as paclitaxel [ 24 , 121 ]. Increased ROS following a piperlongumine treatment diminishes the expression levels of both c-Myc and c-Myc-regulated miRNAs (miR-17, miR-20a, and miR-27a) [ 24 ] ( Figure 1 and Table 3 ).…”

Section: Oncogenic Mirnas Inhibited By Phytochemicals Currently Evmentioning

confidence: 99%

Participation of MicroRNAs in the Treatment of Cancer with Phytochemicals

et al. 2020

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Cancer is a global health concern and one of the main causes of disease-related death. Even with considerable progress in investigations on cancer therapy, effective anti-cancer agents and regimens have thus far been insufficient. There has been compelling evidence that natural phytochemicals and their derivatives have potent anti-cancer activities. Plant-based anti-cancer agents, such as etoposide, irinotecan, paclitaxel, and vincristine, are currently being applied in medical treatments for patients with cancer. Further, the efficacy of plenty of phytochemicals has been evaluated to discover a promising candidate for cancer therapy. For developing more effective cancer therapy, it is required to apprehend the molecular mechanism deployed by natural compounds. MicroRNAs (miRNAs) have been realized to play a pivotal role in regulating cellular signaling pathways, affecting the efficacy of therapeutic agents in cancer. This review presents a feature of phytochemicals with anti-cancer activity, focusing mainly on the relationship between phytochemicals and miRNAs, with insights into the role of miRNAs as the mediators and the regulators of anti-cancer effects of phytochemicals.

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Piperlongumine induces ROS mediated cell death and synergizes paclitaxel in human intestinal cancer cells

Cited by 66 publications

References 45 publications

Cigarette Smoke Exposure Inhibits Osteoclast Apoptosis via the mtROS Pathway

Cigarette Smoke Exposure Inhibits Osteoclast Apoptosis via the mtROS Pathway

One Pot Synthesis of PEGylated Bimetallic Gold–Silver Nanoparticles for Imaging and Radiosensitization of Oral Cancers

Participation of MicroRNAs in the Treatment of Cancer with Phytochemicals

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