PIPPin, a Putative RNA-Binding Protein Specifically Expressed in the Rat Brain

Castiglia, Daniele; Scaturro, Maria; Nastasi, Tommaso; Cestelli, Alessandro; Liegro, Italia Di

doi:10.1006/bbrc.1996.0068

Cited by 30 publications

(45 citation statements)

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“…Fig. 2 also shows H3.3 RNA binding to PIPPin, the RNA-binding activity of which has been previously reported (12,13).…”

Section: Rna-binding Activity Of Pep-19supporting

confidence: 64%

“…In looking for proteins able to bind mRNAs encoding the histone variants H1˚ and H3.3, we identified a set of proteins that bind the one and/or the other histone mRNAs (11). We also cloned a cDNA that encodes a protein, that we called PIPPin (12)(13)(14), now also known as CSD-C2, that contains a cold shock domain and binds both mRNAs at the level of the polyadenylation signal (13).…”

Section: Introductionmentioning

confidence: 99%

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RNA-binding activity of the rat calmodulin-binding PEP-19 protein and of the long PEP-19 isoform

Saladino

Proia²,

Sala³

et al. 2011

Int J Mol Med

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Abstract. Synthesis of H1˚ histone protein, in the developing rat brain, seems to be regulated mainly at the post-transcriptional level. Since regulation of RNA metabolism depends on a series of RNA-binding proteins, we have been searching for RNA-binding proteins involved in the post-transcriptional regulation of the H1˚ gene. We recently reported isolation, from a cDNA expression library, of an insert encoding a novel protein, the C-terminal half of which is identical to that of PEP-19, a brain-specific protein involved in calcium metabolism. The novel protein was called long PEP-19 isoform (LPI). Herein we show that LPI, as well as PEP-19, can bind H1˚ RNA. Moreover, in order to improve production of functional LPI/PEP-19, we modified the protocol normally adopted for preparing histidine tagged-proteins from bacteria, by adding an additional purification step. We also found that both LPI and PEP can compete for H1˚ RNA binding with PIPPin (CSD-C2), another RNA-binding protein previously discovered in our laboratory. Since PEP19/LPI contain a calmodulin binding domain, we finally investigated whether their ability to bind RNA is affected by calmodulin. Our results show that calmodulin interferes with binding of H1˚ RNA to both PEP-19 and LPI, while it is not able to bind RNA on its own. This finding suggests that calcium/calmodulin may have a role in controlling H1˚ mRNA metabolism in the developing brain.

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“…Fig. 2 also shows H3.3 RNA binding to PIPPin, the RNA-binding activity of which has been previously reported (12,13).…”

Section: Rna-binding Activity Of Pep-19supporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

RNA-binding activity of the rat calmodulin-binding PEP-19 protein and of the long PEP-19 isoform

Saladino

Proia²,

Sala³

et al. 2011

Int J Mol Med

Self Cite

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“…We also demonstrate this point by the study of several genes that similarly did not reach an expression ratio of Ͼ2, but the expression of which was markedly increased by hyperosmolality (Table 3). These include the transcription-related genes PIPPin (Castiglia et al, 1996;Raimondi et al, 2003), and LIM only 4 (Kenny et al, 1998;Sugihara et al, 1998). Future experiments will focus on genes exhibiting exceptionally large (in comparison with VP) increases in expression in response to hyperosmolar conditions, such as zinc finger protein 312 (Fez), C1q domain containing 1, EST similar to FLJ20037, opsin, and Rho GD1, because of their potential important roles in regulating OT and VP gene expression in the SON.…”

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confidence: 99%

Selective Gene Expression in Magnocellular Neurons in Rat Supraoptic Nucleus

Mutsuga¹,

Shahar²,

Verbalis³

et al. 2004

J. Neurosci.

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Oxytocin-and vasopressin-producing magnocellular neurons (MCNs) of the hypothalamo-neurohypophysial system are the only neuronal phenotypes present in the rat supraoptic nucleus (SON). Laser microdissection of the SON, extraction and T7-based amplification of its RNAs, and analysis of the resulting cDNAs by hybridization on a 35, 319 element DNA microarray have provided a detailed composite view of the gene expression profile of the MCNs. The genes expressed in the SON were compared with those expressed in a reference tissue consisting of total hypothalamus, and this "expression ratio" indicated which genes were preferentially expressed in the SON. Of the 26,000 unique genes on the array, 1385 were found to be expressed in the SON at levels more than two times greater than in the hypothalamus as a whole. Of these, 123 were expressed Ն3.4-fold higher in the SON versus hypothalamus. Most of these preferentially expressed genes were not previously known to be expressed in the MCNs. Quantitative and double-label in situ hybridization histochemistry was used selectively to confirm a number of these microarray observations and to evaluate the osmotic regulation and cell-specific expression of these genes, respectively.

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“…The present study was aimed at analyzing the effects of NaAsO 2 on primary cultures of rat astrocytes by determining DNA damage by comet assay, and evaluating possible changes of the concentration of the highly conserved heat shock protein 70 (Hsp70) and its housekeeping counterpart, Hsc70. We also investigated the effects of arsenic on PIPPin (also called CSD-C2), a putative RNA-binding protein expressed in the brain and probably involved in the post-transcriptional regulation of genes encoding replacement histones, and in the terminal differentiation of different classes of brain cells (17)(18)(19). PIPPin shuttles between the nucleus and cytoplasm, and can undergo post-translational modifications, such as phosphorylation (18, Di Liegro et al, unpublished obserations) and sumoylation (19), in response to extracellular stimuli.…”

Section: Introductionmentioning

confidence: 99%

Biological effects of inorganic arsenic on primary cultures of rat astrocytes

Catanzaro

Schiera²,

Sciandrello³

et al. 2010

Int J Mol Med

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Abstract. It is well established that inorganic arsenic induces neurotoxic effects and neurological defects in humans and laboratory animals. The cellular and molecular mechanisms of its actions, however, remain elusive. Herein we report the effects of arsenite (NaAsO 2 ) on primary cultures of rat astrocytes. Cells underwent induction of heat shock protein 70 only at the highest doses of inorganic arsenic (30 and 60 μM), suggesting a high threshold to respond to stress. We also investigated arsenic genotoxicity with the comet assay. Interestingly, although cells treated with 10 μM arsenite for 24 h maintained >70% viability, with respect to untreated cells, high DNA damage was already observed. Since arsenic is not known to be a direct-acting genotoxic agent, we investigated the possibility that its effects are due, in astrocytes as well, to ROS formation, as already described for other cell types. However, FACS analysis after CM-H 2 DCFDA staining did not evidence any significant increase of ROS production while, on the contrary, at the highest arsenite concentrations used, ROS production decreased. Concordantly, we found that, if most cells in the culture are still alive (i.e. up to 10 μM arsenite), they show a treatment-dependent increase in the concentration of SOD1. On the other hand, SOD2 concentration did not change. Finally, we found that astrocytes also synthesize PIPPin, an RNA-binding protein, the concentration of which was recently reported to change in response to stress induced by cadmium. Here we also report that, in cells exposed to high doses of arsenite, an anti-PIPPin antibody-positive faster migrating protein appears.

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PIPPin, a Putative RNA-Binding Protein Specifically Expressed in the Rat Brain

Cited by 30 publications

References 0 publications

RNA-binding activity of the rat calmodulin-binding PEP-19 protein and of the long PEP-19 isoform

RNA-binding activity of the rat calmodulin-binding PEP-19 protein and of the long PEP-19 isoform

Selective Gene Expression in Magnocellular Neurons in Rat Supraoptic Nucleus

Biological effects of inorganic arsenic on primary cultures of rat astrocytes

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