piR-55490 inhibits the growth of lung carcinoma by suppressing mTOR signaling

Peng, Liping; Song, Lei; Liu, Chaoying; Lv, Xiaoyi; Li, Xiaoping; Jie, Jing; Zhao, Dan; Li, Dan

doi:10.1007/s13277-015-4056-0

Cited by 96 publications

(100 citation statements)

References 16 publications

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“…Recently, piR-55490 was found to be silenced in lung carcinoma specimens and cell lines, compared with normal lung tissues and cells, and piR-55490 suppression led to the gain in the proliferation rate. Further study revealed that piR-55490 suppressed the activation of Akt/mTOR pathway by binding 3’UTR of mTOR messenger RNA (mRNA) and induce its degradation in a mechanism similar to miRNA in lung cancer cells [79]. These results facilitate our understanding of piRNA’s functions and its role in cancer cell proliferation and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

PIWI Proteins and PIWI-Interacting RNA: Emerging Roles in Cancer

Han

Xia

et al. 2017

Cell Physiol Biochem

127

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P-Element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) are a type of noncoding RNAs (ncRNAs) and interact with PIWI proteins. piRNAs were primarily described in the germline, but emerging evidence revealed that piRNAs are expressed in a tissue-specific manner among multiple human somatic tissue types as well and play important roles in transposon silencing, epigenetic regulation, gene and protein regulation, genome rearrangement, spermatogenesis and germ stem-cell maintenance. PIWI proteins were first discovered in Drosophila and they play roles in spermatogenesis, germline stem-cell maintenance, self-renewal, retrotransposons silencing and the male germline mobility control. A growing number of studies have demonstrated that several piRNA and PIWI proteins are aberrantly expressed in various kinds of cancers and may probably serve as a novel biomarker and therapeutic target for cancer treatment. Nevertheless, their specific mechanisms and functions need further investigation. In this review, we discuss about the biogenesis, functions and the emerging role of piRNAs and PIWI proteins in cancer, providing novel insights into the possible applications of piRNAs and PIWI proteins in cancer diagnosis and clinical treatment.

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Section: Introductionmentioning

confidence: 99%

PIWI Proteins and PIWI-Interacting RNA: Emerging Roles in Cancer

Han

Xia

et al. 2017

Cell Physiol Biochem

127

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“…The role of the piRNA–PIWI protein complex in post-transcriptional gene regulation is also slowly garnering attention. Although the exact mechanism remains elusive, investigators initially have reported the sequence specific complementary binding of a piRNA to a target messenger RNA (mRNA) at the 3′ untranslated region (UTR) and subsequent gene regulation, in a manner similar to that of miRNAs [17–19]. It is increasingly being recognized that the sequence based complementarity may not be restricted to 3′ UTR and may expand to 5′UTR, the coding sequence or even the introns [20].…”

Section: Introductionmentioning

confidence: 99%

Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer

et al. 2016

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Piwi-interacting RNAs (piRNAs), whose role in germline maintenance has been established, are now also being classified as post-transcriptional regulators of gene expression in somatic cells. PIWI proteins, central to piRNA biogenesis, have been identified as genetic and epigenetic regulators of gene expression. piRNAs/PIWIs have emerged as potential biomarkers for cancer but their relevance to breast cancer has not been comprehensively studied. piRNAs and mRNAs were profiled from normal and breast tumor tissues using next generation sequencing and Agilent platforms, respectively. Gene targets for differentially expressed piRNAs were identified from mRNA expression dataset. piRNAs and PIWI genes were independently assessed for their prognostic significance (outcomes: Overall Survival, OS and Recurrence Free Survival, RFS). We discovered eight piRNAs as novel independent prognostic markers and their association with OS was confirmed in an external dataset (The Cancer Genome Atlas). Further, PIWIL3 and PIWIL4 genes showed prognostic relevance. 306 gene targets exhibited reciprocal relationship with piRNA expression. Cancer cell pathways such as apoptosis and cell signaling were the key Gene Ontology terms associated with the regulated gene targets. Overall, we have captured the entire cascade of events in a dysregulated piRNA pathway and have identified novel markers for breast cancer prognostication.

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“…In fact, piR-55490 was recently reported to be downregulated in lung cancer patient specimens and to be significantly associated with poor overall survival. Moreover, piR-55490 was shown to function as a potent tumor suppressor by inducing degradation of mTOR mRNA in a miRNA-like manner and thereby suppressing the activation of the Akt/mTOR pathway, leading to inhibition of lung cancer cell proliferation [28]. …”

Section: Introductionmentioning

confidence: 99%

Small RNA sequencing reveals metastasis-related microRNAs in lung adenocarcinoma

et al. 2017

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The majority of lung cancer deaths are caused by metastatic disease. MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression and miRNA dysregulation can contribute to metastatic progression. Here, small RNA sequencing was used to profile the miRNA and piwi-interacting RNA (piRNA) transcriptomes in relation to lung cancer metastasis. RNA-seq was performed using RNA extracted from formalin-fixed paraffin embedded (FFPE) lung adenocarcinomas (LAC) and brain metastases from 8 patients, and LACs from 8 patients without detectable metastatic disease. Impact on miRNA and piRNA transcriptomes was subtle with 9 miRNAs and 8 piRNAs demonstrating differential expression between metastasizing and non-metastasizing LACs. For piRNAs, decreased expression of piR-57125 was the most significantly associated with distant metastasis. Validation by RT-qPCR in a LAC cohort comprising 52 patients confirmed that decreased expression of miR-30a-3p and increased expression of miR-210-3p were significantly associated with the presence of distant metastases. miR-210-3p tumor cell specificity was evaluated by in situ hybridization and its biomarker potential was confirmed by ROC curve analysis (AUC = 0.839). Lastly, agreement between miRNA-seq and RT-qPCR for FFPE-derived RNA was evaluated and a high level of concordance was determined. In conclusion, this study has identified and validated metastasis-related miRNAs in LAC.

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piR-55490 inhibits the growth of lung carcinoma by suppressing mTOR signaling

Cited by 96 publications

References 16 publications

PIWI Proteins and PIWI-Interacting RNA: Emerging Roles in Cancer

PIWI Proteins and PIWI-Interacting RNA: Emerging Roles in Cancer

Piwi-interacting RNAs and PIWI genes as novel prognostic markers for breast cancer

Small RNA sequencing reveals metastasis-related microRNAs in lung adenocarcinoma

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