Pirbuterol A Preliminary Review of its Pharmacological Properties and Therapeutic Efficacy in Reversible Bronchospastic Disease

Richards, David M.; Brogden, Rex N.

doi:10.2165/00003495-198530010-00002

Cited by 8 publications

(1 citation statement)

References 32 publications

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“…Additionally, widely utilized drugs, such as crotamiton (for treating scabies and itching), enterodiol sulfate (modulates the immune system in humans and may have protective roles against the development of chronic diseases), valsartan (for lowering high blood pressure), metharbital (anti-convulsant), etomidate (anesthetic agent), ozagrel (antiplatelet agent), pirbuterol (bronchodilator), and many amino acid derivatives were putatively identified at different spectra in X. perforans, suggesting that the microbe is a drug bank of potentially medically relevant molecules [67,[127][128][129][130].…”

Section: Pathway Analysis Of Significant Metabolites Of X Perforansmentioning

confidence: 99%

Metabolomics Insights into Chemical Convergence in Xanthomonas perforans and Metabolic Changes Following Treatment with the Small Molecule Carvacrol

et al. 2021

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Microbes are natural chemical factories and their metabolome comprise diverse arrays of chemicals. The genus Xanthomonas comprises some of the most important plant pathogens causing devastating yield losses globally and previous studies suggested that species in the genus are untapped chemical minefields. In this study, we applied an untargeted metabolomics approach to study the metabolome of a globally spread important xanthomonad, X. perforans. The pathogen is difficult to manage, but recent studies suggest that the small molecule carvacrol was efficient in disease control. Bacterial strains were treated with carvacrol, and samples were taken at time intervals (1 and 6 h). An untreated control was also included. There were five replicates for each sample and samples were prepared for metabolomics profiling using the standard procedure. Metabolomics profiling was carried out using a thermo Q-Exactive orbitrap mass spectrometer with Dionex ultra high-performance liquid chromatography (UHPLC) and an autosampler. Annotation of significant metabolites using the Metabolomics Standards Initiative level 2 identified an array of novel metabolites that were previously not reported in Xanthomonas perforans. These metabolites include methoxybrassinin and cyclobrassinone, which are known metabolites of brassicas; sarmentosin, a metabolite of the Passiflora-heliconiine butterfly system; and monatin, a naturally occurring sweetener found in Sclerochiton ilicifolius. To our knowledge, this is the first report of these metabolites in a microbial system. Other significant metabolites previously identified in non-Xanthomonas systems but reported in this study include maculosin; piperidine; β-carboline alkaloids, such as harman and derivatives; and several important medically relevant metabolites, such as valsartan, metharbital, pirbuterol, and ozagrel. This finding is consistent with convergent evolution found in reported biological systems. Analyses of the effect of carvacrol in time-series and associated pathways suggest that carvacrol has a global effect on the metabolome of X. perforans, showing marked changes in metabolites that are critical in energy biosynthesis and degradation pathways, amino acid pathways, nucleic acid pathways, as well as the newly identified metabolites whose pathways are unknown. This study provides the first insight into the X. perforans metabolome and additionally lays a metabolomics-guided foundation for characterization of novel metabolites and pathways in xanthomonad systems.

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