Pirfenidone inhibits TGF-β expression in malignant glioma cells

Burghardt, Isabel; Tritschler, Felix; Opitz, Christiane A.; Frank, Brigitte; Weller, Michael; Wick, Wolfgang

doi:10.1016/j.bbrc.2007.01.012

Cited by 60 publications

(51 citation statements)

References 26 publications

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“…The differentiation medium was composed of Dulbecco's modified Eagle's medium (DMEM) and 3% (v/v) horse serum (Gibco). Pirfenidone (Selleck, USA; 20 μg/ml; Burghardt et al., 2007) was used to stimulate the satellite cells continuously for 24 hr.…”

Section: Methodsmentioning

confidence: 99%

Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development

Zhang

et al. 2018

Aging Cell

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SummarySatellite cells play a key role in the aging, generation, and damage repair of skeletal muscle. The molecular mechanism of satellite cells in these processes remains largely unknown. This study systematically investigated for the first time the characteristics of mouse satellite cells at ten different ages. Results indicated that the number and differentiation capacity of satellite cells decreased with age during skeletal muscle development. Transcriptome analysis revealed that 2,907 genes were differentially expressed at six time points at postnatal stage. WGCNA and GO analysis indicated that 1,739 of the 2,907 DEGs were mainly involved in skeletal muscle development processes. Moreover, the results of WGCNA and protein interaction analysis demonstrated that Tgfβ2, Wnt9a, and Fgfr4 were the key genes responsible for the differentiation of satellite cells. Functional analysis showed that TGFβ2 and WNT9a inhibited, whereas FGFR4 promoted the differentiation of satellite cells. Furthermore, each two of them had a regulatory relationship at the protein level. In vivo study also confirmed that TGFβ2 could regulate the regeneration of skeletal muscle, as well as the expression of WNT9a and FGFR4. Therefore, we concluded that the synergistic effects of TGFβ2, WNT9a, and FGFR4 were responsible for attenuating of the differentiation of aging satellite cells during skeletal muscle development. This study provided new insights into the molecular mechanism of satellite cell development. The target genes and signaling pathways investigated in this study would be useful for improving the muscle growth of livestock or treating muscle diseases in clinical settings.

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Section: Methodsmentioning

confidence: 99%

Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development

Zhang

et al. 2018

Aging Cell

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“…Lysates for immunoblots and cell culture supernatants were prepared as described previously (27,28). Briefly, supernatants were generated for 72 hours after plating 3 Â 10 6 cells in serum-free medium.…”

Section: Methodsmentioning

confidence: 99%

Costimulatory Protein 4IgB7H3 Drives the Malignant Phenotype of Glioblastoma by Mediating Immune Escape and Invasiveness

Lemke

Pfenning

Sahm

et al. 2012

Clinical Cancer Research

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120

101

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Purpose: Recent work points out a role of B7H3, a member of the B7-family of costimulatory proteins, in conveying immunosuppression and enforced invasiveness in a variety of tumor entities. Glioblastoma is armed with effective immunosuppressive properties resulting in an impaired recognition and ineffective attack of tumor cells by the immune system. In addition, extensive and diffuse invasion of tumor cells into the surrounding brain tissue limits the efficacy of local therapies. Here, 4IgB7H3 is assessed as diagnostic and therapeutic target for glioblastoma.Experimental Design: To characterize B7H3 in glioblastoma, we conduct analyses not only in glioma cell lines and glioma-initiating cells but also in human glioma tissue specimens.Results: B7H3 expression by tumor and endothelial cells correlates with the grade of malignancy in gliomas and with poor survival. Both soluble 4IgB7H3 in the supernatant of glioma cells and cell-bound 4IgB7H3 are functional and suppress natural killer cell-mediated tumor cell lysis. Gene silencing showed that membrane and soluble 4IgB7H3 convey a proinvasive phenotype in glioma cells and glioma-initiating cells in vitro. These proinvasive and immunosuppressive properties were confirmed in vivo by xenografted 4IgB7H3 gene silenced glioma-initiating cells, which invaded significantly less into the surrounding brain tissue in an orthotopic model and by subcutaneously injected LN-229 cells, which were more susceptible to natural killer cell-mediated cytotoxicity than unsilenced control cells.Conclusions: Because of its immunosuppressive and proinvasive function, 4IgB7H3 may serve as a therapeutic target in the treatment of glioblastoma.

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“…Likewise, the TGF-b inhibitor perfenidone decreased MMP-11, but not MMP-2, in malignant glioma cells (61). However, earlier studies with human myometrial cells demonstrated an inhibition of MMP-1 and MMP-3 with TGF-b1 treatment (26).…”

Section: Figurementioning

confidence: 96%

Myometrial cells undergo fibrotic transformation under the influence of transforming growth factor β-3

Joseph

Malik

Nurudeen

et al. 2010

Fertility and Sterility

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Pirfenidone inhibits TGF-β expression in malignant glioma cells

Cited by 60 publications

References 26 publications

Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development

Synergistic effects of TGFβ2, WNT9a, and FGFR4 signals attenuate satellite cell differentiation during skeletal muscle development

Costimulatory Protein 4IgB7H3 Drives the Malignant Phenotype of Glioblastoma by Mediating Immune Escape and Invasiveness

Myometrial cells undergo fibrotic transformation under the influence of transforming growth factor β-3

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