Piroplasms of New Zealand seabirds

Paparini, Andrea; McInnes, Linda M.; Placido, Daniela Di; Mackereth, Graham; Tompkins, Daniel M.; Clough, Richard W.; Ryan, Una; Irwin, Peter

doi:10.1007/s00436-014-4118-z

Cited by 20 publications

(22 citation statements)

References 37 publications

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“…Characterization of the mitochondrial genome and parasite features of Babesia bicornis , a species grouped in the T . equi clade based on 18S analysis [7,46], will likely aid in the resolution of the evolutionary relationships of this clade. Lack of evidence for host leukocyte manipulation by T .…”

Section: Discussionmentioning

confidence: 99%

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Mitochondrial Genome Sequences and Structures Aid in the Resolution of Piroplasmida phylogeny

et al. 2016

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The taxonomy of the order Piroplasmida, which includes a number of clinically and economically relevant organisms, is a hotly debated topic amongst parasitologists. Three genera (Babesia, Theileria, and Cytauxzoon) are recognized based on parasite life cycle characteristics, but molecular phylogenetic analyses of 18S sequences have suggested the presence of five or more distinct Piroplasmida lineages. Despite these important advancements, a few studies have been unable to define the taxonomic relationships of some organisms (e.g. C. felis and T. equi) with respect to other Piroplasmida. Additional evidence from mitochondrial genome sequences and synteny should aid in the inference of Piroplasmida phylogeny and resolution of taxonomic uncertainties. In this study, we have amplified, sequenced, and annotated seven previously uncharacterized mitochondrial genomes (Babesia canis, Babesia vogeli, Babesia rossi, Babesia sp. Coco, Babesia conradae, Babesia microti-like sp., and Cytauxzoon felis) and identified additional ribosomal fragments in ten previously characterized mitochondrial genomes. Phylogenetic analysis of concatenated mitochondrial and 18S sequences as well as cox1 amino acid sequence identified five distinct Piroplasmida groups, each of which possesses a unique mitochondrial genome structure. Specifically, our results confirm the existence of four previously identified clades (B. microti group, Babesia sensu stricto, Theileria equi, and a Babesia sensu latu group that includes B. conradae) while supporting the integration of Theileria and Cytauxzoon species into a single fifth taxon. Although known biological characteristics of Piroplasmida corroborate the proposed phylogeny, more investigation into parasite life cycles is warranted to further understand the evolution of the Piroplasmida. Our results provide an evolutionary framework for comparative biology of these important animal and human pathogens and help focus renewed efforts toward understanding the phylogenetic relationships within the group.

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Section: Discussionmentioning

confidence: 99%

“…equi within Theileria [8–10], and in fact, the majority of recent molecular phylogenetic analyses of 18S demonstrate that T . equi represents a unique clade within Piroplasmida (Fig 1; [7,11,24,45,46]). Furthermore, two of these studies have placed T .…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Genome Sequences and Structures Aid in the Resolution of Piroplasmida phylogeny

et al. 2016

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“…Recently, the description of piroplasmids in avian species around the world has been raised [9][10][11][12][48][49][50][51][52]. According to Chavatte et al [53] avian piroplasmids form a poliphyletic group that is currently divided into three clades: the first one is related to Babesia duncani and protheilerids and formed by B. ardeae from Ardea cinerea, B. poelea and Fig.…”

Section: Discussionmentioning

confidence: 99%

“…comprises another group of protozoa associated with performance decreasing in birds [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Arthropod-borne agents in wild Orinoco geese ( Neochen jubata ) in Brazil

Werther

Luzzi

Gonçalves

et al. 2017

Comparative Immunology, Microbiology and Infectious Diseases

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Although Orinoco goose (Neochen jubata) is an anatid species widely distributed in South America, scarce are the reports on the occurrence of arthropod-borne pathogens in this avian species. The present work aimed to verify, by serological and molecular methods, the occurrence of haemosporida piroplasmids and Anaplasmataceae agents in wild Orinoco geese captured in Brazil. Between 2010 and 2014, 62 blood samples were collected from free-living geese captured in the Araguaia River, Goiás State, Brazil. Six geese (10%) were seropositive for Anaplasma phagocytophilum, showing titers ranging from 40 and 80. Twenty out of 62 blood samples (32.25%) were positive in nested PCR for hemosporidia (cytochrome b gene). Fifteen and five sequences shared identity with Haemoproteus and Plasmodium, respectively. Six out of 62 blood samples (9.68%) were positive in nested PCR for Babesia spp. (18S rRNA gene); one sequence showed to be closely related to Babesia vogeli. Thirty (48.38%) out of 62 Orinoco geese blood samples were positive in nested cPCR assays for Anaplasmataceae agents (16S rRNA gene): three for Anaplasma spp. and 27 for Ehrlichia. Six geese were simultaneously positive to Haemoproteus and Ehrlichia; three animals were co-positive to different Ehrlichia species/genotypes; and one goose sample was positive for both Anaplasma and Ehrlichia. The present work showed the occurrence of Ehrlichia, Anaplasma, Babesia, Plasmodium, and Haemoproteus species in free-living N. jubata in Brazil. The threat of these arthropod-borne pathogens in Orinoco goose's fitness, especially during the breading season, should be assessed in the future.

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“…The PCR conditions for the primary PCR (primers BT18SF1/BT18SR1) consisted of a pre-PCR step of 95 °C for 5 min; followed by 40 cycles of 94 °C for 30 s, 52 °C for 30 s, and 72 °C for 2 min; and a final extension of 72 °C for 7 min. The PCR conditions of the secondary PCR (primers BT18SF2/BT18SR2) consisted of a pre-PCR step of 95 °C for 5 min; followed by 40 cycles of 94 °C for 30 s, 50 °C for 30 s, and 72 °C for 80 s; and a final extension of 72 °C for 7 min (PAPARINI et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

Rangelia vitalii and Hepatozoon canis coinfection in pampas fox Lycalopex gymnocercus from Santa Catarina State, Brazil

Silva

Mattoso

Costa³

et al. 2018

Rev. Bras. Parasitol. Vet.

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Rangelia vitalii is a haemoparasite that infects erythrocytes, white blood cells and the cytoplasm of endothelial cells of blood capillaries of canids in South America, and has been detected in both domestic dogs and sylvatic canids. Hepatozoon canis is a parasite that infects neutrophils and monocytes of many mammalian hosts. This study reports the infection of Lycalopex gymnocercus from Santa Catarina, Brazil, with R. vitalii and H. canis. The piroplasm was observed on both blood smears and molecular tests. Many large piroplasms were detected inside the erythrocytes, with round, oval, or teardrop-shaped organism, that occurred singly or in pairs. They had an abundant, pale blue cytoplasm and decentral dark red small nucleus. The animal was also infected with H. canis that was detected only by molecular tests. The majority of haematological and biochemistry parameters were within the reference values for domestic dog and wild canids.

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Piroplasms of New Zealand seabirds

Cited by 20 publications

References 37 publications

Mitochondrial Genome Sequences and Structures Aid in the Resolution of Piroplasmida phylogeny

Mitochondrial Genome Sequences and Structures Aid in the Resolution of Piroplasmida phylogeny

Arthropod-borne agents in wild Orinoco geese ( Neochen jubata ) in Brazil

Rangelia vitalii and Hepatozoon canis coinfection in pampas fox Lycalopex gymnocercus from Santa Catarina State, Brazil

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